Application of MALDI-TOF mass spectrometry for differentiation of closely related species of the “Arthrobacter crystallopoietes” phylogenetic group

Prisyazhnaya, N. V.; Плотникова, Е. Г.; Bueva, O. V.; Korsakova, E. S.; Dorofeeva, Lubov V.; Ilina, Elena; Lebedev, A. T.; Evtushenko, Lyudmila I.

doi:10.1134/s0026261712060136

Cited by 9 publications

(10 citation statements)

References 25 publications

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“…1). The peak with an m/z of 7107 that was characterized by the highest intensity (I = 100) and was also detected in the spectra of other Arthrobacter species [3] appears to be a chemotaxonomic marker of the genus.…”

Section: Resultsmentioning

confidence: 88%

“…The applicability of MALDI for chemodiagnostics of bacterial intrapopulation variability was tested, assuming the similarity degree of bacterial MALDI profiles reflects the phylogenetic relationship of the organisms involved [3] and, accordingly, increases in the genus-species-strain-variant sequence. We ana lyzed the protein spectra of 7 bacterial strains belong ing to 5 genera, including ancient bacteria that were isolated from the permafrost deposits of Siberia with an age of 200 thousand to 2-3 million years.…”

Section: Resultsmentioning

confidence: 99%

“…Proteomic analysis of the vegetative cells of bacte rial variants using MALDI was carried out by the method described in [22] and modified in [3]. Cell samples were obtained by taking aliquots from a liquid culture of a specified age.…”

Section: Methodsmentioning

confidence: 99%

“…Currently, MALDI TOF MS (matrix assisted laser desorption/ionization time of flight mass spec trometry) is increasingly applied to a large number of issues of microbial systematics, such as differentiation between the organisms belonging to closely related genomic species, preliminarily identification of new phenotypically close isolates, and identification and classification of bacteria at the species and the subspe cies levels [1][2][3][4][5][6]. This technique is based upon the mass spectrum analysis of the mass dependent quan titative distribution of the splinters of protein mole cules (m/z).…”

mentioning

confidence: 99%

“…and on the software used to detect the characteristic protein peaks in order to determine the limits within which a bacterium belongs to a specific hierarchical structure and to develop the databases using this information [1,7,8]. Under standardized analytical conditions, this tech nique holds particular promise in terms of quick taxo nomic diagnostics (in conjunction with other meth ods) [9,10], including the differentiation of closely related bacterial species [3] and same species strains [11].…”

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confidence: 99%

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Applicability of MALDI mass spectrometry for diagnostics of phase variants in bacterial populations

et al. 2015

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Efficiency of MALDI mass spectrometry for differentiation between phenotypic phase variants (in colony morphology and virulence/avirulence) was investigated for saprotrophic and opportunistically pathogenic bacteria of five genera (Acinetobacter, Arthrobacter, Rhodococcus, Corynebacterium, and Escheri chia). Analysis of MALDI spectra (on the SA and HCCA matrices) included (1) determination of similarity of the protein spectra as a percentage of the common protein peaks to the total amount of proteins, which reflects the phylogenetic relationships of the objects and has been recommended for identification of closely related species; (2) comparison of intensities of the common peaks; and (3) the presence of specific peaks as determinative characteristics of the variants. Under the standard analytical conditions, the similarity between the MALDI profiles was shown to increase in the row: genus-species-strain-variant. Assessment of inten sities of the common peaks was most applicable for differentiation between phase variants, especially in the case of high similarity of their profiles. Phase variants (A. oxydans strain K14) with similar colony morpho types (S, R, M, and S m ) grown on different media (LB agar, TSA, and TGYg) exhibited differences in their protein profiles reflecting the differences in their physiological characteristics. This finding is in agreement with our previous results on screening of the R. opacus with similar colony morphology and different substrate specificity in decomposition of chlorinated phenols. Analysis of MALDI spectra is probably the only efficient method for detection of such variants.

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Section: Resultsmentioning

confidence: 88%

Section: Resultsmentioning

confidence: 99%