Application of magnetic nanoparticles in smart enzyme immobilization

Vaghari, Hamideh; Jafarizadeh‐Malmiri, Hoda; Mohammadlou, Mojgan; Berenjian, Aydin; Anarjan, Navideh; Jafari, Nahideh; Nasiri, Shahin

doi:10.1007/s10529-015-1977-z

Cited by 306 publications

(165 citation statements)

References 36 publications

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“…In the last years, nanoparticles have been shown to be an immobilization support of great importance due to a substantial increase in their availability and versatility. The use of magnetic particles has been considered as a novel strategy for Smart Immobilization of enzymes via covalent attachment (Vaghari et al, 2015). Moreover, the use of this support in industrial reactors has been greatly encouraged since the appearance of paramagnetic nanoparticles, as recovery and reuse of thismaterial can be accomplished by simply exposing the system to a magnetic field (Gárcia-Galan et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

“…Another important feature of these non-porous supports is the smaller size of the particles, resulting in a reduction of the diffusion hindrance (Zheng et al, 2003).As a result, a significant progress in the last few years has been made in the use of magnetic nanoparticles as carrier for the binding of enzymes. Nevertheless, the immobilized enzymes still present some drawbacks, such as change in properties and low efficacy against insoluble substrates (Vaghari et al, 2015). For this reason, further research is yet needed in order to address these limitations and allow industrial application.…”

Section: Introductionmentioning

confidence: 99%

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Production of flavor esters catalyzed by lipase B from Candida antarctica immobilized on magnetic nanoparticles

Souza

Santos

Freire

et al. 2017

Braz. J. Chem. Eng.

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-Candida antarctica Lipase B (CALB) immobilized onto iron magnetic nanoparticles was evaluated as biocatalyst for the synthesis of flavor esters. Methyl and ethyl butyrate were synthesized by esterification of butyric acid with methanol and ethanol, respectively, in a medium containing solvent. The nanoparticles were produced by the co-precipitation method. The process parameters (type of solvent, temperature, substrate concentration, molar ratio, amount of biocatalyst, stirring speed and reaction time) were studied. The optimum conditions for both esters were achieved at 25 °C, 0.5 mol/L (methyl butyrate) and 0.4 mol/L (ethyl butyrate), molar ratio of 1:1, amount of biocatalyst: 10 mg, 150 rpm and 8 h of reaction, using heptane as solvent. Under those conditions, the maximum conversions of methyl butyrate and ethyl butyrate were higher than 90 %. The synthesis of flavor esters was also conducted by using Novozym® 435, a commercial catalyst, for comparison purposes.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Production of flavor esters catalyzed by lipase B from Candida antarctica immobilized on magnetic nanoparticles

Souza

Santos

Freire

et al. 2017

Braz. J. Chem. Eng.

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“…To overcome these issues, many researchers have studied enzyme immobilization using magnetic nanoparticles, because magnetic nanoparticles can be readily separated the reaction solutions using magnetic attraction [42,52,53,[64][65][66][67][68]. Nanoscale magnetic particles have a unique property of superparamagnetism [69,70]. They do not form agglomerates at room temperature; thus, they are well-suspended in reaction solution [70].…”

Section: Magnetic Nanoparticlesmentioning

confidence: 99%

“…They do not form agglomerates at room temperature; thus, they are well-suspended in reaction solution [70]. The magnetic iron oxides have been mostly used as magnetic nanoparticles because of their low toxicity and biocompatibility [69]. Dyal et al [53] evaluated the activity and stability of Candida rugosa lipase (CRL) attached on γ-Fe 2 O 3 magnetic nanoparticles by covalent binding.…”

Section: Magnetic Nanoparticlesmentioning

confidence: 99%

Nano-Immobilized Biocatalysts for Biodiesel Production from Renewable and Sustainable Resources

Kim

Lee

2018

Catalysts

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Abstract:The cost of biodiesel production relies on feedstock cost. Edible oil is unfavorable as a biodiesel feedstock because of its expensive price. Thus, non-edible crop oil, waste oil, and microalgae oil have been considered as alternative resources. Non-edible crop oil and waste cooking oil are more suitable for enzymatic transesterification because they include a large amount of free fatty acids. Recently, enzymes have been integrated with nanomaterials as immobilization carriers. Nanomaterials can increase biocatalytic efficiency. The development of a nano-immobilized enzyme is one of the key factors for cost-effective biodiesel production. This paper presents the technology development of nanomaterials, including nanoparticles (magnetic and non-magnetic), carbon nanotubes, and nanofibers, and their application to the nano-immobilization of biocatalysts. The current status of biodiesel production using a variety of nano-immobilized lipase is also discussed.

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“…While achieving immobilisation without compromising the functional properties of enzymes is extremely challenging, great progress has been made in this research area [2,3]. Some examples include the use of magnetic nanoparticles [4], coupling purification and immobilisation into a single step [5], and developing a detailed understanding of the ideal physical properties of the solid support [6].In recent years my research has focused on developing an alternative approach to enzyme immobilisation with the goal of developing extremely robust enzymes for industrial applications. Our strategy has been to use a silk protein as an engineering scaffold into which metal cofactors can be incorporated to introduce enzymatic function into the silk materials.…”

mentioning

confidence: 99%

Solid-State Metalloproteins—An Alternative to Immobilisation

Rapson

2016

Molecules

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This commentary outlines a protein engineering approach as an alternative to immobilisation developed in our laboratory. We use a recombinant silk protein into which metal active sites can be incorporated to produce solid-state metalloprotein materials. The silk protein directly coordinates to the metal centres providing control over their reactivity akin to that seen in naturally occurring metalloproteins. These solid-state materials are remarkably stable at a range of temperatures and different solvent conditions. I discuss the genesis of this approach and highlight areas where such solid-state materials could find application.Keywords: biocatalysis; industrial biotechnology; silk; biosensors; de novo engineering Enzymes hold great promise to provide green alternatives from traditional industrial processes. However, in order for an enzyme's full potential to be realised, their use needs to be economically viable. Immobilisation of enzymes is a promising method to reduce the cost of production and processing, as it allows multiple uses of a single batch of enzymes and ease of product separation. In addition, immobilisation can also lead to an improvement of other enzymatic properties such as stability, selectivity, and specificity [1]. While achieving immobilisation without compromising the functional properties of enzymes is extremely challenging, great progress has been made in this research area [2,3]. Some examples include the use of magnetic nanoparticles [4], coupling purification and immobilisation into a single step [5], and developing a detailed understanding of the ideal physical properties of the solid support [6].In recent years my research has focused on developing an alternative approach to enzyme immobilisation with the goal of developing extremely robust enzymes for industrial applications. Our strategy has been to use a silk protein as an engineering scaffold into which metal cofactors can be incorporated to introduce enzymatic function into the silk materials. Hence giving us solid state metalloprotein materials.In the natural world, silks are almost always fibres. Silk proteins however can be fabricated in other material formats such as sponges and films. It was these unusual films that initially got me interested in silks ( Figure 1A).I was a postdoc developing nitric oxide sensors and looking for a new way to immobilise my nitric oxide binding proteins. Another postdoc (Holly Trueman) in the lab was making silk films. We used to joke that her silk films were so flexible we could use them as cling wrap to bring our sandwiches to work. Not only were the films flexible, they were also optically transparent and seemed perfect for use in an optical biosensor.To begin with, we used silks as a way to immobilise heme proteins such as myoglobin. The active site of myoglobin is an iron porphyrin macrocycle, to which gases bind and give these proteins their distinctive colouration. The immobilised myoglobin was still able to reversibly bind nitric oxide. The myoglobin-silk films were remarka...

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Application of magnetic nanoparticles in smart enzyme immobilization

Cited by 306 publications

References 36 publications

Production of flavor esters catalyzed by lipase B from Candida antarctica immobilized on magnetic nanoparticles

Production of flavor esters catalyzed by lipase B from Candida antarctica immobilized on magnetic nanoparticles

Nano-Immobilized Biocatalysts for Biodiesel Production from Renewable and Sustainable Resources

Solid-State Metalloproteins—An Alternative to Immobilisation

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