Application of low intensity mechanical vibrations for bone tissue maintenance and regeneration

Uzan, Melis Olçum; Baskan, Oznur; Karadas, Ozge; Özçivici, Engin

doi:10.3906/biy-1506-76

Cited by 8 publications

(2 citation statements)

References 84 publications

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“…Regeneration of a healthy bone tissue is regulated by chemical and mechanical cues as well as spatial factors and occurs free of scar formation [17,32,35]. However, if the defect size is large, it is considered as Bcritical size bone defect,^and it cannot be healed naturally [14].…”

Section: Introductionmentioning

confidence: 99%

Cytotoxic Tolerance of Healthy and Cancerous Bone Cells to Anti-microbial Phenolic Compounds Depend on Culture Conditions

Karadas

Meşe

Özçivici

2018

Appl Biochem Biotechnol

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Carnosol and carnosic acid are polyphenolic compounds found in rosemary and sage with known anti-oxidant, anti-inflammatory, and anti-microbial properties. Here, we addressed the potential use of carnosol and carnosic acid for in vitro bone tissue engineering applications, specifically depending on their cytotoxic effects on bone marrow stromal and stem cells, and osteosarcoma cells in monolayer and 3D cultures. Carnosol and carnosic acid displayed a bacteriostatic effect on Gram-positive bacteria, especially on S. aureus. The viability results indicated that bone marrow stromal cells and bone marrow stem cells were more tolerant to the presence of carnosol compared to osteosarcoma cells. 3D culture conditions increased this tolerance further for healthy cells, while not affecting the cytotoxic potential of carnosol for osteosarcoma cells. Carnosic acid was found to be more cytotoxic for all cell types used in the study. Results suggest that phenolic compounds might have potential use as anti-microbial and anti-carcinogenic agents for bone tissue engineering with further optimization for controlled release.

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Section: Introductionmentioning

confidence: 99%

Cytotoxic Tolerance of Healthy and Cancerous Bone Cells to Anti-microbial Phenolic Compounds Depend on Culture Conditions

Karadas

Meşe

Özçivici

2018

Appl Biochem Biotechnol

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“…24,25 In addition to these loading variants, daily application of low-intensity (<0.5 g, 1 g = 9.81 m/s 2 ) vibrations (LIVs) in high frequencies (>30 Hz) is anabolic to bone tissue and can suppress obesity in murine and clinical studies. 26–28 LIV can also help maintaining a healthy bone marrow during conditions that are detrimental to bone marrow, such as mechanical disuse 29 or diet-induced obesity. 30 On cellular level, LIV acts as pro-osteogenic to bone marrow stem cells and influences their proliferation, morphology, cell-to-cell communication and molecular markers in vitro 31–33 but cells of other developmental origins, such as epithelial cells, appear to be less sensitive to LIV signals.…”

Section: Introductionmentioning

confidence: 99%

Low-intensity vibrations normalize adipogenesis-induced morphological and molecular changes of adult mesenchymal stem cells

Baskan

Meşe

Özçivici

2017

Proc Inst Mech Eng H

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Bone marrow mesenchymal stem cells that are committed to adipogenesis were exposed daily to high-frequency low-intensity mechanical vibrations to understand molecular, morphological and ultrastructural adaptations to mechanical signals during adipogenesis. D1-ORL-UVA mouse bone marrow mesenchymal stem cells were cultured with either growth or adipogenic medium for 1 week. Low-intensity vibration signals (15 min/day, 90 Hz, 0.1 g) were applied to one group of adipogenic cells, while the other adipogenic group served as a sham control. Cellular viability, lipid accumulation, ultrastructure and morphology were determined with MTT, Oil-Red-O staining, phalloidin staining and atomic force microscopy. Semiquantitative reverse transcription polymerase chain reaction showed expression profile of the genes responsible for adipogenesis and ultrastructure of cells. Low-intensity vibration signals increased viability of the cells in adipogenic culture that was reduced significantly compared to quiescent controls. Low-intensity vibration signals also normalized the effects of adipogenic condition on cell morphology, including area, perimeter, circularization and actin cytoskeleton. Furthermore, low-intensity vibration signals reduced the expression of some adipogenic markers significantly. Mesenchymal stem cells are sensitive and responsive to mechanical loads, but debilitating conditions such as aging or obesity may steer mesenchymal stem cells toward adipogenesis. Here, daily application of low-intensity vibration signals partially neutralized the effects of adipogenic induction on mesenchymal stem cells, suggesting that these signals may provide an alternative and/or complementary option to reduce fat deposition.

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Low-Amplitude and High-Frequency Loading Influences Interstitial Fluid Flow in Osteogenesis Imperfecta Osteon

Shrivas

Tiwari

Tripathi

et al. 2022

Lecture Notes in Mechanical Engineering

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Application of low intensity mechanical vibrations for bone tissue maintenance and regeneration

Cited by 8 publications

References 84 publications

Cytotoxic Tolerance of Healthy and Cancerous Bone Cells to Anti-microbial Phenolic Compounds Depend on Culture Conditions

Cytotoxic Tolerance of Healthy and Cancerous Bone Cells to Anti-microbial Phenolic Compounds Depend on Culture Conditions

Low-intensity vibrations normalize adipogenesis-induced morphological and molecular changes of adult mesenchymal stem cells

Low-Amplitude and High-Frequency Loading Influences Interstitial Fluid Flow in Osteogenesis Imperfecta Osteon

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