Application of loop-mediated isothermal amplification technique to rapid and direct detection of methicillin-resistant Staphylococcus aureus (MRSA) in blood cultures

Misawa, Yoshiki; Yoshida, Atsushi; Saito, Ryoichi; Yoshida, Honami; Okuzumi, Katsuko; Ito, Nobue; Okada, Mitsumasa; Moriya, Kyoji; Koike, Kazuhiko

doi:10.1007/s10156-007-0508-9

Cited by 93 publications

(63 citation statements)

References 20 publications

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“…There are very few studies using LAMP assays for the detection of Staphylococcal isolates, which include femA and mecA genes from food isolates [13], femA from clinical and food samples [14], spa and arc C genes from spiked blood specimens [7], spa and mecA gene from positive blood culture bottles [15], orfX (a highly conserved open reading frame of S.aureus) from clinical isolates [16].…”

Section: Referencesmentioning

confidence: 99%

Evaluation of LAMP Assay Using Phenotypic Tests and Conventional PCR for Detection of nuc and mecA genes Among Clinical Isolates of Staphylococcus spp

Sudhaharan

Vanjari

Neeraja

et al. 2015

JCDR

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Section: Referencesmentioning

confidence: 99%

Evaluation of LAMP Assay Using Phenotypic Tests and Conventional PCR for Detection of nuc and mecA genes Among Clinical Isolates of Staphylococcus spp

Sudhaharan

Vanjari

Neeraja

et al. 2015

JCDR

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“…In addition, Iwamoto et al [9] used the LAMP technology to specific detection of Mycobacterium tuberculosis complex, Mycobacterium avium, and Mycobacterium intracellulure. LAMP has also been successfully used for specific detection of pathogenic microorganisms such as Streptococcus pneumoniae [10], Edwardsiella tarda [11], Edwardsiella ictaluri [12], methicillin-resistant Staphylococcus aureus [13], and Bacillus anthracis [14].…”

Section: Detection Of Bacteria Pathogenic Microorganismsmentioning

confidence: 99%

Applications of Loop-Mediated Isothermal DNA Amplification

Guo

et al. 2010

Appl Biochem Biotechnol

155

104

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During the last 10 years, with the development of loop-mediated isothermal amplification (LAMP) method, it has been widely applied in nucleic acid analysis because of its simplicity, rapidity, high efficiency, and outstanding specificity. This method employs a DNA polymerase and a set of four specially designed primers that recognize a total of six distinct sequences on the target DNA. Expensive equipment are not necessary to acquire a high level of precision, and there are fewer preparation steps compared to conventional PCR and real-time PCR assays. This paper briefly summarized the applications of LAMP method in pathogenic microorganisms, genetically modified ingredients, tumor detection, and embryo sex identification.

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“…It also allows a rapid and direct detection of pathogen variants, such as bacteria resistant to narrow spectrum antibiotics, eg. methicillin [20]. In this study the method proved its high efficiency and good resolution in the detection of oilseed rape pathogens responsible for phoma leaf spotting and stem canker.…”

Section: Discussionmentioning

confidence: 67%

Loop-mediated isothermal amplification as a good tool to study changing Leptosphaeria populations in oilseed rape plants and air samples

Jędryczka

Burzyński

Brachaczek³

et al. 2014

Acta Agrobot

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A b s t r a c t LAMP is an innovative, simple, rapid, specific and cost--effective nucleic acid amplification method. Due to the use of a special enzyme -GspSSD polymerase, the reaction takes a short time and can be performed at isothermal conditions. The sensitivity and specificity of LAMP technique is significantly higher, than standard PCR techniques, as two or three specific primer pairs are used. The technique is regarded as a useful tool for the detection and identification of plant pathogens. In this work, LAMP was used to study the composition of the population of fungi of the genus Leptosphaeria, causing a damaging disease of oilseed rape, called blackleg or stem canker. The detection concerned DNA present in fungal spores contained in air samples obtained using Hirst-type volumetric trap, in Pomerania (north Poland) in 2010. The results achieved using the LAMP technique were similar to these obtained with previously used, highly specific method of Real-time PCR. Conducting LAMP reaction was much easier and less time-and cost-consuming, due to a simplified method of DNA isolation of pathogens from plant tissues. Then, the LAMP technique was used to assess the composition of the population of Leptosphaeria spp. in plants of oilseed rape collected from the field in the Opole region (south-western part of Poland) in 2013. In contrast to studies conducted in [2002][2003], the analysis of leaf symptoms showed a higher proportion of L. maculans compared to L. biglobosa, what reflects changes in the composition of pathogen population of fungi causing blackleg on oilseed rape in this part of Poland.

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Application of loop-mediated isothermal amplification technique to rapid and direct detection of methicillin-resistant Staphylococcus aureus (MRSA) in blood cultures

Cited by 93 publications

References 20 publications

Evaluation of LAMP Assay Using Phenotypic Tests and Conventional PCR for Detection of nuc and mecA genes Among Clinical Isolates of Staphylococcus spp

Evaluation of LAMP Assay Using Phenotypic Tests and Conventional PCR for Detection of nuc and mecA genes Among Clinical Isolates of Staphylococcus spp

Applications of Loop-Mediated Isothermal DNA Amplification

Loop-mediated isothermal amplification as a good tool to study changing Leptosphaeria populations in oilseed rape plants and air samples

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