Application of loop-mediated isothermal amplification assay combined with lateral flow dipstick for detection of Plasmodium falciparum and Plasmodium vivax

Yongkiettrakul, Suganya; Jaroenram, Wansadaj; Arunrut, Narong; Chareanchim, Wanwisa; Pannengpetch, Supicha; Suebsing, Rungkarn; Kiatpathomchai, Wansika; Pornthanakasem, Wichai; Yuthavong, Yongyuth; Kongkasuriyachai, Darin

doi:10.1016/j.parint.2014.06.004

Cited by 54 publications

(30 citation statements)

References 23 publications

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“…[22][23][24][25][26] Our dot-ELISA format is simple and can be handily deployed in the field without specific devices or detection system. However, simpler detection of the mLAMP products, such as by lateral flow dipstick, [27][28][29] will be the most appropriate test for easy use in the field. In fact, recent studies have shown that the detection of mLAMP amplicons by label-based lateral flow dipstick is applicable and adequate for use in pointof-care settings, eliminating the use of skilled personnel.…”

Section: Discussionmentioning

confidence: 99%

Simple Identification of Human Taenia Species by Multiplex Loop-Mediated Isothermal Amplification in Combination with Dot Enzyme-Linked Immunosorbent Assay

Nkouawa¹,

Sako²,

Okamoto³

et al. 2016

The American Journal of Tropical Medicine and Hygiene

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Abstract. For differential detection of Taenia solium, Taenia saginata, and Taenia asiatica, loop-mediated isothermal amplification (LAMP) assay targeting the cytochrome c oxidase subunit 1 gene has been recently developed and shown to be sensitive, specific, and effective. However, to achieve differential identification, one specimen requires three reaction mixtures containing a primer set of each Taenia species separately, which is complex and time consuming and increases the risk of cross-contamination. In this study, we developed a simple differential identification of human Taenia species using multiplex LAMP (mLAMP) in combination with dot enzyme-linked immunosorbent assay (dot-ELISA). Forward inner primers of T. solium, T. saginata, and T. asiatica labeled with fluorescein isothiocyanate (FITC), digoxigenin (DIG), and tetramethylrhodamine (TAMRA), respectively, and biotin-labeled backward inner primers were used in mLAMP. The mLAMP assay succeeded in specific amplification of each respective target gene in a single tube. Furthermore, the mLAMP product from each species was easily distinguished by dot-ELISA with an antibody specific for FITC, DIG, or TAMRA. The mLAMP assay in combination with dot-ELISA will make identification of human Taenia species simpler, easier, and more practical.

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Section: Discussionmentioning

confidence: 99%

Simple Identification of Human Taenia Species by Multiplex Loop-Mediated Isothermal Amplification in Combination with Dot Enzyme-Linked Immunosorbent Assay

Nkouawa¹,

Sako²,

Okamoto³

et al. 2016

The American Journal of Tropical Medicine and Hygiene

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“…Lateral flow dipstick (LFD) was developed as well for simultaneous detection of pathogenic Leptospira spp. (Nurul Najian et al 2016), measles virus (Xu et al 2016), animal babesiosis, caused by Babesia bovis and Babesia bigemina , foot-and-mouth diseases (Waters et al 2014), Japanese encephalitis (Deng et al 2015), P. vivax, and P. falciparum (Yongkiettrakul et al 2014;Kongkasuriyachai et al 2017), Jembrana disease virus (Kusumawati et al 2015), canine parvovirus (Sun et al 2014), Vibrio alginolyticus (Plaon et al 2015), Macrobrachium rosenbergii nodavirus and shrimp yellow head virus (Khunthong et al 2013).…”

Section: Lamp End-point Detection Methodsmentioning

confidence: 99%

Loop-mediated isothermal amplification (LAMP): a versatile technique for detection of micro-organisms

et al. 2018

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“…Different clinical studies have validated this rapid molecular test in the field with a performance similar to conventional PCR [2, 3]. Additional advantages of LAMP are its tolerance to inhibitory substances present in blood samples (such as haemoglobin and immunoglobulin) [4] and the possibility of being used also on small amounts of blood on filter papers. Furthermore, it could be combined with simple techniques such as microwave DNA extraction [5] in basic laboratories in low resource settings.…”

Section: Introductionmentioning

confidence: 99%

LAMP kit for diagnosis of non-falciparum malaria in Plasmodium ovale infected patients

Cuadros

Ramírez

González

et al. 2017

Malar J

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BackgroundMicroscopy and rapid diagnosis tests have a limited sensitivity in diagnosis of malaria by Plasmodium ovale. The LAMP kit (LoopAMP®) can be used in the field without special equipment and could have an important role in malaria control programmes in endemic areas and for malaria diagnosis in returned travellers. The performance of the Pan primer of the kit in detecting malaria by P. ovale was compared with the results of standard nPCR in samples of patients returning from P. ovale endemic areas.Methods Plasmodium ovale positive samples (29, tested by PCR and/or microscopy) and malaria negative specimens (398, tested by microscopy and PCR) were collected in different hospitals of Europe from June 2014 to March 2016 and frozen at −20 °C. Boil and spin method was used to extract DNA from all samples and amplification was performed with LoopAMP® MALARIA kit (Eiken Chemical, Japan) in an automated turbidimeter (Eiken 500). The results of LAMP read by turbidimetry and with the naked eye were compared.ResultsThe kit showed a sensitivity of 100% and a specificity of 97.24% with positive and negative predictive values of 72.5 and 100%, respectively. Naked eyed readings were in accordance with turbidimetry readings (sensitivity, 92.5%, specificity, 98.96% and positive and negative predictive values, respectively, 90.24 and 99.22%). The limit of detection of LAMP assay for P. ovale was between 0.8 and 2 parasites/µl.ConclusionsThe Pan primer of the Malaria kit LoopAMP® can detect P. ovale at very low-levels and showed a predictive negative value of 100%. This tool can be useful in malaria control and elimination programmes and in returned travellers from P. ovale endemic areas. Naked eye readings are equivalent to automated turbidimeter readings in specimens obtained with EDTA.Electronic supplementary materialThe online version of this article (doi:10.1186/s12936-016-1669-8) contains supplementary material, which is available to authorized users.

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Application of loop-mediated isothermal amplification assay combined with lateral flow dipstick for detection of Plasmodium falciparum and Plasmodium vivax

Cited by 54 publications

References 23 publications

Simple Identification of Human Taenia Species by Multiplex Loop-Mediated Isothermal Amplification in Combination with Dot Enzyme-Linked Immunosorbent Assay

Simple Identification of Human Taenia Species by Multiplex Loop-Mediated Isothermal Amplification in Combination with Dot Enzyme-Linked Immunosorbent Assay

Loop-mediated isothermal amplification (LAMP): a versatile technique for detection of micro-organisms

LAMP kit for diagnosis of non-falciparum malaria in Plasmodium ovale infected patients

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