Application of loop mediated isothermal dna amplification to reveal and identify Bacillus anthracis

Shchit, I. Yu.; Ignatov, Konstantin B.; Kudriavtseva, Tamara Y.; Shishkova, N.A.; Mironova, R. I.; Marinin, L.I.; Мокриевич, А. Н.; Kramarov, V. M.; Biketov, S F; Дятлов, И. А.

doi:10.18821/0208-0613-2017-35-2-69-76

Mol. genet. mikrobiol. virusol.

2017

DOI: 10.18821/0208-0613-2017-35-2-69-76

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Application of loop mediated isothermal dna amplification to reveal and identify Bacillus anthracis

I. Yu. Shchit¹,

Konstantin B. Ignatov²,

Tamara Y. Kudriavtseva³

et al.

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2019

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Development and Assessment of a Reagent Kit for Rna Detection of Crimean-Congo Hemorrhagic Fever Virus With Using Reverse Transcription Loop-Mediated Isothermal Amplification Method

Коnonova

Zaykovskaya

et al. 2019

RCLD

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This study presents the results of laboratory trials of the reagent kit for the rapid detection of RNA of the Crimean-Congo hemorrhagic fever virus (CCHFV) using loop-mediated isothermal amplification with reverse transcription (RT-LAMP). The developed RT-LAMP reagent kit was used to detect the CCHFV and showed a sensitivity of 103 GE/ml of viral RNA, which is sufficient for detection of the CCHFV in the early stage of human infections. The kit showed high specificity and no cross-reactivity with viral panel from the State collection of viruses of the FBRI SRC VB «Vector» (arboviruses and hemorrhagic fever viruses). Laboratory trials of the RT-LAMP kit are showed a high analytical and diagnostic sensitivity and specificity for RNA detection of the CCHFV and high speed of the analysis (60-70 min with sample preparation) compared to real-time PCR. Approbation of the kit field version has showed the possibility of setting the RT-LAMP reaction and viral RNA detection without the using of analytical equipments.

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Development and Assessment of a Reagent Kit for Rna Detection of Crimean-Congo Hemorrhagic Fever Virus With Using Reverse Transcription Loop-Mediated Isothermal Amplification Method

Коnonova

Zaykovskaya

et al. 2019

RCLD

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show abstract

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Application of loop mediated isothermal dna amplification to reveal and identify Bacillus anthracis

Cited by 1 publication

References 29 publications

Development and Assessment of a Reagent Kit for Rna Detection of Crimean-Congo Hemorrhagic Fever Virus With Using Reverse Transcription Loop-Mediated Isothermal Amplification Method

Development and Assessment of a Reagent Kit for Rna Detection of Crimean-Congo Hemorrhagic Fever Virus With Using Reverse Transcription Loop-Mediated Isothermal Amplification Method

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