Application of Laser Postionization Secondary Neutral Mass Spectrometry/Time-of-Flight Secondary Ion Mass Spectrometry in Nanotoxicology: Visualization of Nanosilver in Human Macrophages and Cellular Responses

Haase, Andrea; Arlinghaus, Heinrich F.; Tentschert, Jutta; Jungnickel, Harald; Graf, Philipp; Mantion, Alexandre; Draude, Felix; Galla, S.; Plendl, Johanna; Goetz, Mario E.; Mašić, Admir; Meier, Wolfgang; Thünemann, Andreas F.; Taubert, Andreas; Luch, Andreas

doi:10.1021/nn200163w

Cited by 88 publications

(82 citation statements)

References 40 publications

(77 reference statements)

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“…Among other effects, it was demonstrated that carbon-coated silver nanoparticles influence cell viability to a lesser extent than uncoated AgNPs of similar size [95], whereas polysaccharide-coated AgNPs cause more severe effects than uncoated AgNPs [1]. Moreover, it was shown that PVP-coated [66] or peptide-coated [51] AgNPs are more toxic than citrate-coated AgNPs with similar particle core sizes.…”

Section: Toxicity Of Agnpsmentioning

confidence: 99%

Toxic effects of silver nanoparticles in mammals – does a risk of neurotoxicity exist?

Skalska¹,

Strużyńska²

2015

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A b s t r a c t Over the last decade, silver nanoparticles have become an important class of nanomaterials utilized in the development of new nanotechnologies. Despite the fact that nanosilver is used in many commercial applications, our knowledge about its associated risks is incomplete. Although a number of studies have been undertaken to better understand the impact of silver nanoparticles on the environment, aquatic organisms and cell lines, little is known about their side effects in mammalian organisms. This review summarizes relevant data and the current state of knowledge regarding toxicity of silver nanoparticles in mammals, as well as the accumulated evidence for potent neurotoxic effects. The influence of nanosilver on the central nervous system is significant because of evidence indicating that it accumulates in mammalian brain tissue.

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Section: Toxicity Of Agnpsmentioning

confidence: 99%

Toxic effects of silver nanoparticles in mammals – does a risk of neurotoxicity exist?

Skalska¹,

Strużyńska²

2015

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“…The lateral resolution is about 300 nm. Pathological changes in the pattern of cellular lipids following NM exposure have been detected 32, 33. TEM and ToF‐SIMS methods are relatively cost‐intensive and time‐consuming, and so might not be useful as high throughput techniques for NM screening.…”

Section: Label‐free Dosimetry and Imaging Techniques—toward High Thromentioning

confidence: 99%

“…Label‐free imaging of cell organelles,42, 43 uptake and intracellular fate of drug carriers44, 45, 46 and NMs inside individual cells32, 47, 48 have been studied.…”

Section: Label‐free Dosimetry and Imaging Techniques—toward High Thromentioning

confidence: 99%

High throughput toxicity screening and intracellular detection of nanomaterials

Collins

Annangi

Rubio

et al. 2016

WIREs Nanomed Nanobiotechnol

112

106

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With the growing numbers of nanomaterials (NMs), there is a great demand for rapid and reliable ways of testing NM safety—preferably using in vitro approaches, to avoid the ethical dilemmas associated with animal research. Data are needed for developing intelligent testing strategies for risk assessment of NMs, based on grouping and read‐across approaches. The adoption of high throughput screening (HTS) and high content analysis (HCA) for NM toxicity testing allows the testing of numerous materials at different concentrations and on different types of cells, reduces the effect of inter‐experimental variation, and makes substantial savings in time and cost. HTS/HCA approaches facilitate the classification of key biological indicators of NM‐cell interactions. Validation of in vitro HTS tests is required, taking account of relevance to in vivo results. HTS/HCA approaches are needed to assess dose‐ and time‐dependent toxicity, allowing prediction of in vivo adverse effects. Several HTS/HCA methods are being validated and applied for NM testing in the FP7 project NANoREG, including Label‐free cellular screening of NM uptake, HCA, High throughput flow cytometry, Impedance‐based monitoring, Multiplex analysis of secreted products, and genotoxicity methods—namely High throughput comet assay, High throughput in vitro micronucleus assay, and γH2AX assay. There are several technical challenges with HTS/HCA for NM testing, as toxicity screening needs to be coupled with characterization of NMs in exposure medium prior to the test; possible interference of NMs with HTS/HCA techniques is another concern. Advantages and challenges of HTS/HCA approaches in NM safety are discussed. WIREs Nanomed Nanobiotechnol 2017, 9:e1413. doi: 10.1002/wnan.1413For further resources related to this article, please visit the WIREs website.

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“…Most of these particles are neutral. In most SIMS instruments, only secondary ions can be detected unless special measures are taken to achieve postionization of neutral particles ( 24 ). Recent modifi cations of SIMS instruments have focused on increasing sputtering yield and ionization effi ciency to achieve higher sensitivity, increasing the precision of the mass spectrometer, and improving spatial resolution ( 25,26 ).…”

Section: Nanosims Imagingmentioning

confidence: 99%

High-resolution imaging of dietary lipids in cells and tissues by NanoSIMS analysis

Jiang

Goulbourne

Tatar

et al. 2014

Journal of Lipid Research

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This article is available online at http://www.jlr.org within cells and tissues is important, but the experimental approaches for creating high-resolution images of lipids within cells and tissues are quite limited. The most commonly used technique for lipid imaging in tissues is fl uorescence microscopy after the delivery of fl uorescently labeled lipids ( 2, 3 ). A drawback of that approach is that some lipids are not readily available as fl uorescent compounds, and one must worry about how a fl uorescent tag affects the biological properties of the lipid. To overcome these issues, label-free lipid imaging techniques have been developed, such as coherent anti-Stokes Raman scattering microscopy ( 4 ) and stimulated Raman scattering microscopy ( 5 ), but the lateral resolution and sensitivity of these approaches are limited ( 6 ).More recently, imaging MS has been utilized to visualize lipids ( 7,8 ). New ionization methods, primary ion beams, and newer instrument designs have made imaging MS more powerful. MALDI techniques, TOF secondary ion MS (SIMS), and magnetic sector SIMS represent complementary techniques for lipid imaging ( 9 ). In that order, these techniques have increasing spatial resolution but decreasing molecular specifi city. MALDI techniques are able to identify and quantify lipids with a resolution of a few micrometers; TOF-SIMS retains the ability to identify specifi c lipids and can achieve 0.5-1 µm lateral resolution. By measuring the elemental composition of lipids, a nanoscale SIMS (NanoSIMS) instrument yields images of lipids with up to 50 nm lateral resolution. We describe the use of NanoSIMS imaging to create high-resolution images of lipids in cells and tissues.Abstract Nanoscale secondary ion MS (NanoSIMS) imaging makes it possible to visualize stable isotope-labeled lipids in cells and tissues at 50 nm lateral resolution. Here we report the use of NanoSIMS imaging to visualize lipids in mouse cells and tissues. After administering stable isotope-labeled fatty acids to mice by gavage, NanoSIMS imaging allowed us to visualize neutral lipids in cytosolic lipid droplets in intestinal enterocytes, chylomicrons at the basolateral surface of enterocytes, and lipid droplets in cardiomyocytes and adipocytes. After an injection of stable isotope-enriched triglyceriderich lipoproteins (TRLs), NanoSIMS imaging documented delivery of lipids to cytosolic lipid droplets in parenchymal cells. Using a combination of backscattered electron (BSE) and NanoSIMS imaging, it was possible to correlate the chemical data provided by NanoSIMS with high-resolution BSE images of cell morphology. This combined imaging approach allowed us to visualize stable isotope-enriched TRLs along the luminal face of heart capillaries and the lipids within heart capillary endothelial cells. We also observed examples of TRLs within the subendothelial spaces of heart capillaries. NanoSIMS imaging provided evidence of defective transport of lipids from the plasma LPs to adipocytes and cardiomyocytes in mice defi cient in glycosyl...

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Application of Laser Postionization Secondary Neutral Mass Spectrometry/Time-of-Flight Secondary Ion Mass Spectrometry in Nanotoxicology: Visualization of Nanosilver in Human Macrophages and Cellular Responses

Cited by 88 publications

References 40 publications

Toxic effects of silver nanoparticles in mammals – does a risk of neurotoxicity exist?

Toxic effects of silver nanoparticles in mammals – does a risk of neurotoxicity exist?

High throughput toxicity screening and intracellular detection of nanomaterials

High-resolution imaging of dietary lipids in cells and tissues by NanoSIMS analysis

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