Application of laser-accelerated protons to the demonstration of DNA double-strand breaks in human cancer cells

Yogo, Akifumi; Sato, Kazuo; Nishikino, Masaharu; Mori, M.; Teshima, Teruki; Numasaki, Hodaka; Murakami, M.; Demizu, Yusuke; Akagi, Seiko; Nagayama, Shin; Ogura, Koichi; Sagisaka, A.; Orimo, Satoshi; Nishiuchi, Mamiko; Pirozhkov, Alexander S.; Ikegami, Machiko; Tampo, M.; Sakaki, H.; Suzuki, Masayuki; Daito, I.; Oishi, Yuji; Sugiyama, Hironori; Kiriyama, Hiromitsu; Okada, Hajime; Kanazawa, Shuhei; Kondo, Shuji; Shimomura, Takuya; Nakai, Yoshiki; Tanoue, Manabu; Sasao, H.; Wakai, Daisuke; Bolton, Paul R.; Daido, Hiroyuki

doi:10.1063/1.3126452

Cited by 126 publications

(91 citation statements)

References 13 publications

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“…This work is building on previous work from our group [22] and the radiobiological results are consistent with first experiments performed by Yogo et al [24,25] and a recent single-pulse study of the RBE by Doria et al [26] with retrospective dose evaluation.…”

Section: Introductionsupporting

confidence: 85%

Dose-controlled irradiation of cancer cells with laser-accelerated proton pulses

et al. 2012

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Proton beams are a promising tool for the improvement of radiotherapy of cancer, and compact laserdriven proton radiation (LDPR) is discussed as an alternative to established large-scale technology facilitating wider clinical use. Yet, clinical use of LDPR requires substantial development in reliable beam generation and transport, but also in dosimetric protocols as well as validation in radiobiological studies. Here, we present the first dose-controlled direct comparison of the radiobiological effectiveness of intense proton pulses from a laser-driven accelerator with conventionally generated continuous proton beams, demonstrating a first milestone in translational research. Controlled dose delivery, precisely online and offline monitored for each out of *4,000 pulses, resulted in an unprecedented relative dose uncertainty of below 10 %, using approaches scalable to the next translational step toward radiotherapy application.

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Section: Introductionsupporting

confidence: 85%

Dose-controlled irradiation of cancer cells with laser-accelerated proton pulses

et al. 2012

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“…In the meanwhile, several groups have started preliminary work on the methodology and viability of using laser driven ion source for cell irradiation experiments. [6][7][8] The main aim of these investigations is to establish a procedure for cell handling, irradiation and dosimetry 9 compatible with a laser-plasma interaction environment. Furthermore, one of the peculiarities of laser-driven ion beams is their ultrashort duration, as ions are emitted in bursts of picosecond duration at the source and their therapeutic use may result in dose rates many orders of magnitude higher than normally used.…”

Section: Introductionmentioning

confidence: 99%

Biological effectiveness on live cells of laser driven protons at dose rates exceeding 109 Gy/s

Doria¹,

Kakolee²,

Kar³

et al. 2012

AIP Advances

111

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The ultrashort duration of laser-driven multi-MeV ion bursts offers the possibility of radiobiological studies at extremely high dose rates. Employing the TARANIS Terawatt laser at Queen’s University, the effect of proton irradiation at MeV-range energies on live cells has been investigated at dose rates exceeding 109 Gy/s as a single exposure. A clonogenic assay showed consistent lethal effects on V-79 live cells, which, even at these dose rates, appear to be in line with previously published results employing conventional sources. A Relative Biological Effectiveness (RBE) of 1.4±0.2 at 10% survival is estimated from a comparison with a 225kKVp X-ray source

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“…For example, one unique feature of a laser-driven proton source is the potential for extremely high current in a single ion bunch by virtue of both high bunch charge and short duration. To address the fundamental question we have developed a laser-driven ion irradiation apparatus [98][99][100], which was the first attempt to investigate the radiobiological effects for laser-accelerated ions. For example, we have demonstrated DNA double-strand breaking of human cancer cells (as seen in Figure 19) by laser-driven proton bunches of short duration and high single bunch current.…”

Section: Radiation Biology With Laser-accelerated Proton Beamsmentioning

confidence: 99%

Ultra-Intense, High Spatio-Temporal Quality Petawatt-Class Laser System and Applications

et al. 2013

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This paper reviews techniques for improving the temporal contrast and spatial beam quality in an ultra-intense laser system that is based on chirped-pulse amplification (CPA). We describe the design, performance, and characterization of our laser system, which has the potential for achieving a peak power of 600 TW. We also describe OPEN ACCESSAppl. Sci. 2013, 3 215 applications of the laser system in the relativistically dominant regime of laser-matter interactions and discuss a compact, high efficiency diode-pumped laser system.

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Application of laser-accelerated protons to the demonstration of DNA double-strand breaks in human cancer cells

Cited by 126 publications

References 13 publications

Dose-controlled irradiation of cancer cells with laser-accelerated proton pulses

Dose-controlled irradiation of cancer cells with laser-accelerated proton pulses

Biological effectiveness on live cells of laser driven protons at dose rates exceeding 109 Gy/s

Ultra-Intense, High Spatio-Temporal Quality Petawatt-Class Laser System and Applications

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