Application of Langmuir–Blodgett technology for the analysis of saturated fatty acids using the MALDI-TOF mass spectrometry

Podolskaya, Е. P.; Serebryakova, Marina V.; Краснов, К. А.; Grachev, S. A.; Gzgzyan, Alexander M.; Суходолов, Н. Г.

doi:10.1016/j.mencom.2018.05.037

Cited by 7 publications

(14 citation statements)

References 14 publications

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“…The analyses revealed nine fatty acid signals in total, which were annotated in the both species by their exact m/z values with a mass accuracy better than 10 ppm ( Table 1 ), i.e., within the instrument specifications. Thereby, the quality and absolute intensities of the acquired spectra ( Figure 3 ) were comparable with those observed earlier with collapsed solid monolayers of saturated fatty acids [ 21 ] and real extracts obtained from different biological objects including root nodules of pea ( Pisum sativum L.) [ 25 ]. It is important to note, however, that lysis under aqueous conditions ( Figure 3 A,B) yielded approximately doubled spectral intensities and better signal to noise ratios in comparison to those obtained after hexane lysis ( Figure 3 C,D and Table S1 –2).…”

Section: Resultssupporting

confidence: 83%

“…Although the soft reaction conditions make Langmuir technology a promising tool in sample preparation for mass spectrometric analysis, its implementation in analytical practice required, however, principal changes in the monolayer preparation strategy. First, as we have shown in our recent work [ 21 ], the cationic component needs to be replaced by a divalent metal, ideally barium. Indeed, these cations form mostly monocarboxylates and no mixed salts, i.e., yielding efficiently ionizable monolayers.…”

Section: Experimental Designmentioning

confidence: 99%

“…These bottlenecks of GC-MS- and LC-MS-based approaches for FA analysis can be efficiently overcome by implementation of matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS) in combination with Langmuir–Blodgett technology [ 21 ]. Recently, we have shown that Langmuir–Blodgett films can be not only efficiently implemented in selective enrichment of phosphopeptides [ 22 ], protein adducts of organophosphorous compounds [ 23 ] and chlorinated insecticides [ 24 ], but also proved to be an efficient tool for high-throughput and sensitive fingerprinting of free fatty acids as their barium monocarboxylates in positive ion mode [ 25 ].…”

Section: Introductionmentioning

confidence: 99%

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High-Throughput Fingerprinting of Rhizobial Free Fatty Acids by Chemical Thin-Film Deposition and Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry

Gladchuk

Shumilina

Kusnetsova

et al. 2020

MPs

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Fatty acids (FAs) represent an important class of metabolites, impacting on membrane building blocks and signaling compounds in cellular regulatory networks. In nature, prokaryotes are characterized with the most impressing FA structural diversity and the highest relative content of free fatty acids (FFAs). In this context, nitrogen-fixing bacteria (order Rhizobiales), the symbionts of legumes, are particularly interesting. Indeed, the FA profiles influence the structure of rhizobial nodulation factors, required for successful infection of plant root. Although FA patterns can be assessed by gas chromatography—(GC-) and liquid chromatography—mass spectrometry (LC-MS), sample preparation for these methods is time-consuming and quantification suffers from compromised sensitivity, low stability of derivatives and artifacts. In contrast, matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS) represents an excellent platform for high-efficient metabolite fingerprinting, also applicable to FFAs. Therefore, here we propose a simple and straightforward protocol for high-throughput relative quantification of FFAs in rhizobia by combination of Langmuir technology and MALDI-TOF-MS featuring a high sensitivity, accuracy and precision of quantification. We describe a step-by-step procedure comprising rhizobia culturing, pre-cleaning, extraction, sample preparation, mass spectrometric analysis, data processing and post-processing. As a case study, a comparison of the FFA metabolomes of two rhizobia species—Rhizobium leguminosarum and Sinorhizobium meliloti, demonstrates the analytical potential of the protocol.

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Section: Resultssupporting

confidence: 83%

Section: Experimental Designmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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High-Throughput Fingerprinting of Rhizobial Free Fatty Acids by Chemical Thin-Film Deposition and Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry

Gladchuk

Shumilina

Kusnetsova

et al. 2020

MPs

View full text Add to dashboard Cite

show abstract

“…Although the soft reaction conditions make Langmuir technology a promising tool in sample preparation for mass spectrometric analysis, its implementation in analytical practice required, however, principal changes in the monolayer preparation strategy. First, as we have shown in our recent work [21], the cationic component needs to be replaced by divalent metal, ideally barium. Indeed, these cations form mostly monocarboxylates and no mixed salts, i.e.…”

Section: Experimental Designmentioning

confidence: 95%

“…These bottle necks of GC-MS-and LC-MS-based approaches for FA analysis can be efficiently overcome by implementation of matrix-assisted laser desorption/ionization time-of flight mass spectrometry (MALDI-TOF-MS) in combination with Langmuir-Blodgett technology [21]. Recently, we have shown that Langmuir-Blodgett films can be not only efficiently implemented in selective enrichment of phosphopeptides [22], protein adducts of organophosphorous compounds [23] and chlorinated insecticides [24], but also proved to be an efficient tool for high-throughput and sensitive fingerprinting of free fatty acids as their barium monocarboxylates in positive ion mode [25].…”

Section: Figure 1 Structural Class Of Bacterial Fatty Acidsmentioning

confidence: 99%

A High-throughput Method for Fingerprinting of Rhizobial Free Fatty Acids by the Combination of a Thin Film Chemical Deposition Technique and Matrix-assisted Laser Desorption/ionization Time-of Flight Mass Spectrometry

Gladchuk¹,

Schumilina²,

Kusnetsova³

et al. 2020

Preprint

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Fatty acids (FAs) represent an important class of metabolites, impacting on membrane building blocks and cellular regulatory networks. In nature, prokaryotes are characterized with the most impressing FA structural diversity and the highest relative contents of free fatty acids (FFAs). Thereby, nitrogen-fixing bacteria (order Rhizobiales), often found in symbiosis with legumes, attract a special interest. Indeed, FAs impact on the structure of rhizobial nodulation factors, required for successful infection of plant root. Although the FA patterns can be addressed by GC- and LC-MS, these methods are time-consuming and suffer from compromised sensitivity, low stability of derivatives and artifacts. In contrast, MALDI-TOF-MS represents an excellent platform for high-efficient metabolite fingerprinting, also applicable to FFAs. Therefore, here we propose a simple and straightforward protocol for high-throughput relative quantification of FFAs in rhizobia by the combination of Langmuir technology and MALDI-TOF-MS, which is featured with high sensitivity, accuracy and precision of quantification. Here we propose a step by step procedure comprising rhizobia culturing, pre-cleaning, extraction, sample preparation, mass spectrometric analysis, data processing and post-processing. To demonstrate the analytical potential of the protocol we illustrate it by a case study – comparison the FFA metabolomes of two rhizobia species - Rhizobium leguminosarum and Sinorhizobium meliloti.

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A new approach for analysis of polyprenols by a combination of thin‐film chemical deposition and matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry

Gladchuk

Краснов²,

Keltsieva

et al. 2021

Rapid Comm Mass Spectrometry

Self Cite

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Rationale The polyprenols are involved in some essential biosynthetic pathways and serve as ubiquitous components of cellular membranes, so their fingerprinting in natural samples is of great interest. Previous studies indicate that due to the high hydrophobicity of polyprenols their direct analysis by mass spectrometry with soft ionization techniques may be difficult and require preliminary off‐line derivatization. Hence, a method for rapid and sensitive screening of polyprenols is required. Methods A combination of thin‐film chemical deposition and matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI‐TOFMS) was used for analysis of the polyprenol profile of Abies sibirica L. extract. Polyprenol‐based monolayers were formed at the interphase of aqueous barium acetate solution, supplemented with 2,5‐dihydroxybenzoic acid, and an n‐hexane solution of polyprenols directly on a MALDI target plate. Results Peaks corresponding to [M − H + Ba]+ ions were observed in the MALDI‐TOF mass spectra of polyprenols. A total of nine polyprenol homologues were identified with a polyprenol of 16 isoprene units dominating. The limit of detection was established at the level of 6 pg. Possible mechanisms of formation of [M − H + Ba]+ ions of polyprenols were discussed. Conclusions The proposed approach can be suitable for high‐throughput screening of polyprenols in biological samples of different origin due to easy sample preparation and high sensitivity.

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Application of Langmuir–Blodgett technology for the analysis of saturated fatty acids using the MALDI-TOF mass spectrometry

Cited by 7 publications

References 14 publications

High-Throughput Fingerprinting of Rhizobial Free Fatty Acids by Chemical Thin-Film Deposition and Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry

High-Throughput Fingerprinting of Rhizobial Free Fatty Acids by Chemical Thin-Film Deposition and Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry

A High-throughput Method for Fingerprinting of Rhizobial Free Fatty Acids by the Combination of a Thin Film Chemical Deposition Technique and Matrix-assisted Laser Desorption/ionization Time-of Flight Mass Spectrometry

A new approach for analysis of polyprenols by a combination of thin‐film chemical deposition and matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry

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