Application of Lab-on-Chip for Detection of Microbial Nucleic Acid in Food and Environment

Abstract

Various diseases caused by food-borne or environmental pathogenic microorganisms have been a persistent threat to public health and global economies. It is necessary to regularly detect microorganisms in food and environment to prevent infection of pathogenic microorganisms. However, most traditional detection methods are expensive, time-consuming, and unfeasible in practice in the absence of sophisticated instruments and trained operators. Point-of-care testing (POCT) can be used to detect microorganisms rapidly on site and greatly improve the efficiency of microbial detection. Lab-on-chip (LOC) is an emerging POCT technology with great potential by integrating most of the experimental steps carried out in the laboratory into a single monolithic device. This review will primarily focus on principles and techniques of LOC for detection of microbial nucleic acid in food and environment, including sample preparation, nucleic acid amplification and sample detection.

Lab on a Chip Device for Diagnostic Evaluation and Management in Chronic Renal Disease: A Change Promoting Approach in the Patients’ Follow Up

“…LOC systems are miniaturized devices aimed to perform one or several analyses, normally carried out in a laboratory, in a single chip. LOC systems have a wide application range [ 1 , 2 ], including diagnosis (COVID-19) [ 3 ], clinical biochemistry (glucose, INR, blood gases measurements), nucleic acid detection [ 4 ], cell biology research (organ on a chip) [ 5 ], biomedical tissue engineering, environmental sampling, food safety [ 6 , 7 ], natural products discovery, and overproduction [ 8 ].…”

Development and application of a universal extraction-free reagent based on an algal glycolipid

“…It is especially suitable for the rapid on-site molecular detection of pathogens and POCTs. [6][7][8][9] However, nucleic acid analysis by isothermal amplication technology generally consists of three steps: nucleic acid extraction, amplication, and product detection. Among them, nucleic acid extraction is a key step for rapid detection in the eld, but its complexity and high time consumption hinder the application of isothermal amplication technology in rapid pathogen detection and POCT.…”