Application of kDNA as a molecular marker to analyse Leishmania infantum diversity in Portugal

Cortes, Sofia; Maurício, Isabel; Almeida, Ana Claudia Silva; Cristóvão, José Manuel; Pratlong, Francine; Dédet, J. P.; Campino, Lenea

doi:10.1016/j.parint.2006.07.003

Cited by 41 publications

(70 citation statements)

References 25 publications

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“…Although genotypes based on the kDNA minicircle have been used extensively throughout the literature for the purpose of strain identification [45][46][47][48] by extreme variations, conducting this type of procedure is an exceptionally detailed process, which limits its use for parasite genotyping. 49 Figure 2.…”

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confidence: 99%

An Assessment of the Genetic Diversity of Leishmania infantum Isolates from Infected Dogs in Brazil

Batista

Segatto²,

Guedes³

et al. 2012

The American Journal of Tropical Medicine and Hygiene

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Abstract. Correlations between the genetic diversity of Leishmania infantum (syn. L. chagasi) isolates and their respective geographic origins support the theoretic assumption that visceral leishmaniasis probably originated in the Old World. Because dogs are widely considered to be the main reservoir of this disease, the present study aimed to investigate the degree of genetic divergence among 44 leishmanial canine isolates from two Brazilian cities, Jequié and Campo Grande, located approximately 2,028 km from each other. We hypothesized that a low degree of genetic divergence would be observed among these isolates. In fact, statistical analyses found no significant differences between the isolates using both random amplified polymorphic DNA and multilocus microsatellite typing genotyping techniques with three and seven markers, respectively. These findings provide support for the recent introduction of L. infantum into the New World.

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confidence: 99%

An Assessment of the Genetic Diversity of Leishmania infantum Isolates from Infected Dogs in Brazil

Batista

Segatto²,

Guedes³

et al. 2012

The American Journal of Tropical Medicine and Hygiene

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“…The samples were purified with phenol: chloroform: active amyl alcohol at a ratio of 25:24:1 (Invitrogen  ), precipitated with 100% ethanol and 5 M ammonium acetate (SAMBROOK et al, 1989). The samples were quantified in NanoDrop2000 (Thermo Fisher Scientific, Waltham, MA, USA), standardized and amplified with 10 pmoles of specific primers MC1 5' -GTTAGCCGATGGTGGTCTTG-3´ and MC2 5' -CACCCATTTTTCCGATTTTG-3' (CORTES et al, 2006) Nei's coefficient (NEI, 1972). The Neighbor-joining method, with 10,000 bootstrap replications, was used in the cluster analysis.…”

Section: Methodsmentioning

confidence: 99%

“…Different regions of the genetic code of the parasite can be analyzed by using several different genetic markers. Polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) analysis techniques have allowed the identification and evaluation of the genetic structure of populations and strains of Leishmania spp., revealing the presence of different genotypes and polymorphisms in strains isolated in Brazil and in Europe (CORTES et al, 2006;ALONSO et al, 2010). These factors, may be attributed to known genetic events, as well as, external environmental pressures (LAINSON & SHAW, 1987).…”

Section: Introductionmentioning

confidence: 99%

Genetic variability of Leishmania infantum in naturally infected dogs in the state of Bahia, Brazil

Carvalho

Albuquerque

Carneiro

et al. 2017

Rev. Bras. Parasitol. Vet.

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In Brazil, Leishmania infantum mainly affects humans and dogs. The state of Bahia presents many dogs that are positive for this parasite. Despite the importance of epidemiology in this region, there are still very few studies that have assessed the genetic characteristics of L. infantum. The aim of this study is to investigate the genetic variability of L. infantum isolated identified in naturally infected dogs, in order to verify occurrence of subpopulation of this parasite in the different biomes existing in the state of Bahia. Thirty-two samples of L. infantum were analyzed, which were obtained isolated in dogs from the Mata Atlântica (rainforest), Caatinga (semi-arid scrub forest), and Cerrado (a vast tropical savannah eco-region) Bahia municipalities' biomes. All animals presented with clinical changes suggestive of Leishmania spp. and they exhibited positive reactions to serological tests. kDNA analysis with RFLP markers revealed the presence of genetic variability and gene flow in subpopulations of L. infantum; samples from the Mata Atlântica areas were genetically more similar to those from the areas of Caatinga and they were less likely to resemble those of the Cerrado. This data may be used to investigate the dissemination of parasite in the canine population of state of Bahia.Keywords: Canine Leishmaniasis, PCR-RFLP, populations, enzyme, zoonosis. ResumoNo Brasil a Leishmania infantum afeta principalmente o homem e os cães. O estado da Bahia apresenta elevado número de cães positivos por este parasito. Apesar da importância epidemiologia para a região, ainda há poucos estudos que avaliam as características genéticas de L. infantum. Objetivou-se com este estudo investigar a variabilidade genética de cepas de L. infantum identificadas em cães naturalmente infectados, a fim de verificar a ocorrência de sobpopulações do parasito nos diferentes biomas existentes no estado da Bahia. Foram analisadas 32 amostras de L. infantum isoladas em cães de municípios baianos distribuídos nos biomas Mata Atlântica, Caatinga e Cerrado. Todos os animais apresentavam alterações clínicas sugestivas de Leishmania spp. e reação positiva em exames sorológicos. A análise do kDNA com marcadores RFLP revelaram a presença de variabilidade genética e fluxo gênico nas subpopulações de L. infantum sendo que as amostras das áreas de Mata Atlântica foram geneticamente mais semelhantes as das áreas de Caatinga e foram mais distantes daquelas oriundas do Cerrado. Estas informações podem auxiliar em investigações de dispersão do parasito na população canino do Estado da Bahia.

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“…Entretanto, a classificação de L. (L.) chagasi tem sido contestada por estudos de cepas do complexo L. donovani isoladas de diferentes origens, utilizando marcadores moleculares que incluem: sequências codificantes e não-codificantes de DNA nuclear e/ou mitocondrial, espaçador transcrito interno do gene ribosomal (ITS) (CUPOLILLO et al, 1995;DÁVILA e MOMEN, 2000;KUHLS et al, 2005), amplificação aleatória de DNA polimórfico (RAPD) (HIDE et al, 2001), análise de polimorfismo de fragmentos de restrição (RFLP) de minicírculo de kDNA (ALONSO et al, 2010;CORTES et al, 2006), tipagem de microssatélites multilocus (MLMT) , amplificação do gene mini-exon (SUKMEE et al, 2008) e da proteína de choque térmico 70 kDa (hsp70) (FRAGA et al, 2010), entre outros.…”

Section: As Classificações Iniciais Propostas Para Os Parasitos Perteunclassified

Capacidade vetorial de Lutzomyia (Lutzomyia) cruzi (Diptera: Psychodidae) para Leishmania (Leishmania) infantum

Oliveira¹

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In some regions such as in the municipalities of Corumbá and Ladário in Mato Grosso do Sul state, there are ecological and epidemiological evidence that Lutzomyia (Lutzomyia) cruzi (Mangabeira, 1938) is the vector of Leishmania cruzi specimens, since this species represent 93.94% of the total captured. The monthly change showed that Lu. cruzi species has great plasticity and has been observed in all months of collection.

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Application of kDNA as a molecular marker to analyse Leishmania infantum diversity in Portugal

Cited by 41 publications

References 25 publications

An Assessment of the Genetic Diversity of Leishmania infantum Isolates from Infected Dogs in Brazil

An Assessment of the Genetic Diversity of Leishmania infantum Isolates from Infected Dogs in Brazil

Genetic variability of Leishmania infantum in naturally infected dogs in the state of Bahia, Brazil

Capacidade vetorial de Lutzomyia (Lutzomyia) cruzi (Diptera: Psychodidae) para Leishmania (Leishmania) infantum

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