Application of jasmonic acid at the stage of visible brown necrotic spots in Magnaporthe oryzae infection as a novel and environment-friendly control strategy for rice blast disease

Wang, Yunfeng; Duan, Guihua; Li, Chunqin; Ma, Xinrong; Yang, Jing

doi:10.1007/s00709-020-01591-0

Cited by 9 publications

(6 citation statements)

References 58 publications

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“…The results suggest that a synergistic effect of SA and JA exists under certain conditions in rice [43]. Jasmonate is required for defense against the hemibiotrophic pathogen M. oryzae [21], and application of JA after M. oryzae infection can alleviate the disease symptoms [44]. It is reasonable to expect that the activated JA signaling pathway in sah2-3KO plants positively regulates the resistance to necrotrophic pathogens, such as R. solani and B. oryzae (Figure 4c,d, Figures S8 and S9).…”

Section: Discussionmentioning

confidence: 90%

Salicylic Acid Is Required for Broad-Spectrum Disease Resistance in Rice

Bian

Wang

Yang

et al. 2022

IJMS

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Rice plants contain high basal levels of salicylic acid (SA), but some of their functions remain elusive. To elucidate the importance of SA homeostasis in rice immunity, we characterized four rice SA hydroxylase genes (OsSAHs) and verified their roles in SA metabolism and disease resistance. Recombinant OsSAH proteins catalyzed SA in vitro, while OsSAH3 protein showed only SA 5-hydroxylase (SA5H) activity, which was remarkably higher than that of other OsSAHs that presented both SA3H and SA5H activities. Amino acid substitutions revealed that three amino acids in the binding pocket affected SAH enzyme activity and/or specificity. Knockout OsSAH2 and OsSAH3 (sahKO) genes conferred enhanced resistance to both hemibiotrophic and necrotrophic pathogens, whereas overexpression of each OsSAH gene increased susceptibility to the pathogens. sahKO mutants showed increased SA and jasmonate levels compared to those of the wild type and OsSAH-overexpressing plants. Analysis of the OsSAH3 promoter indicated that its induction was mainly restricted around Magnaporthe oryzae infection sites. Taken together, our findings indicate that SA plays a vital role in immune signaling. Moreover, fine-tuning SA homeostasis through suppression of SA metabolism is an effective approach in studying broad-spectrum disease resistance in rice.

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Section: Discussionmentioning

confidence: 90%

Salicylic Acid Is Required for Broad-Spectrum Disease Resistance in Rice

Bian

Wang

Yang

et al. 2022

IJMS

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“…Our study together with others shows that melatonin is an effective antifungal and biofungicidal adjuvant for M. oryzae and over 10 other plant pathogens 9,10,13,73,74 . Plant metabolites such as chitosan, jasmonic acid and pipernonaline also have the inhibition effects on M. oryzae 75–77 . Though environment‐friendly, natural small molecules alone often cannot fully satisfy the practical application in the field, the combination of melatonin and isoprothiolane demonstrated here has the potential to solve the above shortcoming of natural molecules in disease control.…”

Section: Discussionmentioning

confidence: 65%

“…9,10,13,73,74 Plant metabolites such as chitosan, jasmonic acid and pipernonaline also have the inhibition effects on M. oryzae. [75][76][77] Though environment-friendly, natural small molecules alone often cannot fully satisfy the practical application in the field, the combination of melatonin and isoprothiolane demonstrated here has the potential to solve the above shortcoming of natural molecules in disease control. The synergistic effect of melatonin and isoprothiolane in controlling rice blast are in agreement with observations that drug synergism can improve antifungal efficiency, reduce drug dosage and expand drug targets, possibly leading to delayed drug resistance.…”

Section: Discussionmentioning

confidence: 99%

Melatonin targets MoIcl1 and works synergistically with fungicide isoprothiolane in rice blast control

et al. 2023

Journal of Pineal Research

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Melatonina natural harmless molecule-displays versatile roles in human health and crop disease control such as for rice blast. Rice blast, caused by the filamentous fungus Magnaporthe oryzae, is one devastating disease of rice. Application of fungicides is one of the major measures in the control of various crop diseases. However, fungicide resistance in the pathogen and relevant environmental pollution are becoming serious problems. By screening for possible synergistic combinations, here, we discovered an eco-friendly combination for rice blast control, melatonin, and the fungicide isoprothiolane. These compounds together exhibited significant synergistic inhibitory effects on vegetative growth, conidial germination, appressorium formation, penetration, and plant infection by M. oryzae. The combination of melatonin and isoprothiolane reduced the effective concentration of isoprothiolane by over 10-fold as well as residual levels of isoprothiolane. Transcriptomics and lipidomics revealed that melatonin and isoprothiolane synergistically interfered with lipid metabolism by regulating many common targets, including the predicted isocitrate lyaseencoding gene MoICL1. Furthermore, using different techniques, we show that melatonin and isoprothiolane interact with MoIcl1. This study demonstrates that melatonin and isoprothiolane function synergistically and can be used to reduce the dosage and residual level of isoprothiolane, potentially contributing to the environment-friendly and sustainable control of crop diseases.

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“…In this study, we identified some genes related to jasmonic acid (JA), ethylene (ET), auxin, and abscisic acid (ABA) ( Figure 6D ). OsAOS2 ( allene oxide synthase 2 ), OsAOS3 ( allene oxide synthase 3 ), OsOPR7 ( OPDA reductase 7 ), and JIOsPR10 ( jasmonate inducible pathogenesis-related class 10 ), all of which figure prominently in the biosynthesis of JA, were up-regulated dramatically, which could be viewed as resistance response to pathogens ( Wang et al., 2021 ). SIT1 ( salt intolerance 1 ), OsERF101 ( ethylene response factor 101 ), OsERF62 ( ethylene response factor 62 ), Sub1C ( submergence1C ), OsETR2 ( ethylene response 2 ), OsACS2 ( ACC synthase 2 ), and OsACO5 ( ACC oxidase 5 ), which participate in ethylene (ET) biosynthesis and response ( Helliwell et al., 2016 ), were increased significantly at the transcript level.…”

Section: Resultsmentioning

confidence: 99%

Knockout of a papain-like cysteine protease gene OCP enhances blast resistance in rice

Liu

Mei

et al. 2022

Front. Plant Sci.

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Papain-like cysteine proteases (PLCPs) play an important role in the immune response of plants. In Arabidopsis, several homologous genes are known to be involved in defending against pathogens. However, the effects of PLCPs on diseases that afflict rice are largely unknown. In this study, we show that a PLCP, an oryzain alpha chain precursor (OCP), the ortholog of the Arabidopsis protease RD21 (responsive to dehydration 21), participates in regulating resistance to blast disease with a shorter lesion length characterizing the knockout lines (ocp-ko), generated via CRISPR/Cas9 technology. OCP was expressed in all rice tissues and mainly located in the cytoplasm. We prove that OCP, featuring cysteine protease activity, interacts with OsRACK1A (receptor for activated C kinase 1) and OsSNAP32 (synaptosome-associated protein of 32 kD) physically in vitro and in vivo, and they co-locate in the rice cytoplasm but cannot form a ternary complex. Many genes related to plant immunity were enriched in the ocp-ko1 line whose expression levels changed significantly. The expression of jasmonic acid (JA) and ethylene (ET) biosynthesis and regulatory genes were up-regulated, while that of auxin efflux transporters was down-regulated in ocp-ko1. Therefore, OCP negatively regulates blast resistance in rice by interacting with OsRACK1A or OsSNAP32 and influencing the expression profiles of many resistance-related genes. Moreover, OCP might be the cornerstone of blast resistance by suppressing the activation of JA and ET signaling pathways as well as promoting auxin signaling pathways. Our research provides a comprehensive resource of PLCPs for rice plants in defense against pathogens that is also of potential breeding value.

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Application of jasmonic acid at the stage of visible brown necrotic spots in Magnaporthe oryzae infection as a novel and environment-friendly control strategy for rice blast disease

Cited by 9 publications

References 58 publications

Salicylic Acid Is Required for Broad-Spectrum Disease Resistance in Rice

Salicylic Acid Is Required for Broad-Spectrum Disease Resistance in Rice

Melatonin targets MoIcl1 and works synergistically with fungicide isoprothiolane in rice blast control

Knockout of a papain-like cysteine protease gene OCP enhances blast resistance in rice

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