Application of iodine-azide reaction for detection of amino acids in thin-layer chromatography

Kaźmierczak, Dorota; Ciesielski, Witold; Zakrzewski, Robert; Zuber, Monika

doi:10.1016/j.chroma.2004.10.009

Cited by 19 publications

(12 citation statements)

References 2 publications

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“…It is very difficult to visualize the amino acids by the UV lamp method and considerable efforts have been made to detect them on the TLC surface by the dyeing reagent [2][3][4] or coupling TLC with other techniques such as MS . We have applied the TLC-IMS method for a mixture of the amino acids alanine and isoleucine.…”

Section: Analysis Of the Mixtures Of Amino Acidsmentioning

confidence: 99%

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Laser desorption-ion mobility spectrometry as a useful tool for imaging of thin layer chromatography surface

Ilbeigi

Sabo

Valadbeigi

et al. 2016

Journal of Chromatography A

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Section: Analysis Of the Mixtures Of Amino Acidsmentioning

confidence: 99%

“…Traditionally, the analyte spots on the TLC plate are visualized by chemical and optical methods such as staining techniques and dyeing reagents [2][3][4] ultraviolet (UV) absorption and fluorescence spectroscopy [5].…”

Section: Introductionmentioning

confidence: 99%

Laser desorption-ion mobility spectrometry as a useful tool for imaging of thin layer chromatography surface

Ilbeigi

Sabo

Valadbeigi

et al. 2016

Journal of Chromatography A

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“…A reaction of iodine with sodium azide named "iodineazide reaction" is another means to detect amino acids in the TLC technique [18]. Uses of molecular iodine include addi tives (23%), Xray contrast media (22%), germicides and disinfectants (17%), catalysts (15%), pharmaceuticals (9%), and other (14%) [19].…”

Section: Introductionmentioning

confidence: 99%

Molecular iodine/polymer complexes

Moulay

2013

Journal of Polymer Engineering

163

127

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A unique feature of molecular iodine by far, is its ability to bind to polymeric materials. A plethora of natural and synthetic polymers develop complexes when treated with molecular iodine, or with a mixture of mole cular iodine and potassium iodide. Many unexpected findings have been encountered upon complexation of iodine and the polymer skeleton, including the color formation, the polymer morphology changes, the compl exation sites or regions, the biological activity, and the electrical conductivity enhancement of the complexes, with poly iodides (I n¯) , mainly I 3¯ and I 5¯, as the actual binding species. Natural polymers that afford such complexes with iodine species are starch (amylose and amylopectin), chitosan, glycogen, silk, wool, albumin, cellulose, xylan, and natural rubber; iodinestarch being the oldest iodinenatural polymer complex. By contrast, numerous synthetic polymers are prone to make com plexes, including poly(vinyl alcohol) (PVA), poly(vinyl pyrrolidone) (PVP), nylons, poly(Schiff base)s, poly aniline, unsaturated polyhydrocarbons (carbon nano tubes, fullerenes C 60 /C 70 , polyacetylene; iodinePVA being the oldest iodinesynthetic polymer complex.

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“…Following the pattern of in situ PITC amino acid derivatization, [23] the research into the biogenic amines was completed. The main goal was to establish optimal conditions considering PITC derivatization in situ on the normal phase TLC plates.…”

Section: Derivatizationmentioning

confidence: 99%

“…The procedure involving this derivatization together with the iodine-azide detection proved to be beneficial for the amino acids analyses. [22,23] Thus, it was interesting to extend the research to biogenic amines. Moreover, up to this point there have been no reports considering in situ PITC derivatization of biogenic amines in TLC.…”

Section: Introductionmentioning

confidence: 99%

Application of the Iodine‐Azide Procedure for Detection of Biogenic Amines in TLC

Kaźmierczak

Ciesielski

Zakrzewski

2006

Journal of Liquid Chromatography & Related Technologies

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The iodine-azide reaction was employed for TLC detection of sulphur containing derivatives of biogenic amines. The derivatization reaction with phenyl isothiocyanate (PITC) took place directly on the plate before the developing step. Subsequently, the plates were sprayed with a mixture of sodium azide and starch solution in NP-TLC and, in the case of RP-TLC, sodium azide solution with starch was incorporated into the mobile phase and then exposed to iodine vapour. The spots became visible as white spots on violet grey background. The results of the detection limits proved to be advantageous to other commonly used detection techniques (UV and iodine chamber). The attempt to separate the mixtures of phenyl thiocarbamyl biogenic amines was also made.

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Application of iodine-azide reaction for detection of amino acids in thin-layer chromatography

Cited by 19 publications

References 2 publications

Laser desorption-ion mobility spectrometry as a useful tool for imaging of thin layer chromatography surface

Laser desorption-ion mobility spectrometry as a useful tool for imaging of thin layer chromatography surface

Molecular iodine/polymer complexes

Application of the Iodine‐Azide Procedure for Detection of Biogenic Amines in TLC

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