Application of internal standard method in recombinant luminescent bacteria test

Wang, Yongzhi; Li, Dan; He, Mengchang

doi:10.1016/j.jes.2015.03.021

Cited by 5 publications

(2 citation statements)

References 34 publications

(33 reference statements)

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“…In 1985, Engebrecht first used the lux gene as a bioreporter [63]. Subsequently, researchers applied the promoter-less lux-CDABE to produce the broad-spectrum host range pUCD615 vector, which is widely used in the production of RLB [64].…”

Section: Rlb As Reportersmentioning

confidence: 99%

Recent advances in the development and applications of luminescent bacteria–based biosensors

Li,

Zhao,

et al. 2024

Luminescence

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Luminescent bacteria–based biosensors are widely used for fast and sensitive monitoring of food safety, water quality, and other environmental pollutions. Recent advancements in biomedical engineering technology have led to improved portability, integration, and intelligence of these biotoxicity assays. Moreover, genetic engineering has played a significant role in the development of recombinant luminescent bacterial biosensors, enhancing both detection accuracy and sensitivity. This review provides an overview of recent advances in the development and applications of novel luminescent bacteria–based biosensors, and future perspectives and challenges in the cutting‐edge research, market translation, and practical applications of luminescent bacterial biosensing are discussed.

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Section: Rlb As Reportersmentioning

confidence: 99%

Recent advances in the development and applications of luminescent bacteria–based biosensors

Li,

Zhao,

et al. 2024

Luminescence

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show abstract

“…The results showed that the half effect mass concentration (EC50) of genetic recombinant luminescent bacteria E. coli HB101 pUCD607 was higher than Vibrio fischeri, which indicated that the recombinant luminescent bacteria were more sensitive than Vibrio fischeri in detecting metal toxicity in the soil. Wang Yongzhi et al constructed luminescent bacteria with mercury-sensitive and anti-mercury genes to detect the toxicity of mercury ions, and the results showed that the recombinant strains had high sensitivity and specificity for detecting soil pollution with mercury ions [94]. He Wei et al introduced fusion fragments (mer-egfp and nah-luxCDABE) carrying regulatory gene elements and reporter genes into host cells to construct recombinant luminescent bacteria.…”

Section: Recombinant Luminescent Bacteria Bioassay In the Detection O...mentioning

confidence: 99%

Application of Luminescent Bacteria Bioassay in the Detection of Pollutants in Soil

et al. 2023

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The luminescent bacteria bioassay has been commonly used in the detection of environmental pollutants. Compared with traditional chemical and other biological detection methods, the luminescent bacteria bioassay has many demonstrated advantages such as a sensitive response, low cost, high efficiency, and environmental friendliness. The traditional luminescent bacteria bioassay has poor reproducibility and cannot achieve undisturbed soil testing, and the use of leach liquor also affects the results. This paper reviews the research progress and existing issues for the traditional luminescent bacteria bioassay used in the detection of soil pollutants. The luminescence mechanisms and detection principles of three commonly used luminescent bacteria, i.e., Vibrio fischeri, Photobacterium phosphoreum, and Vibrio qinghaiensis, are discussed and compared. In addition, two new luminescent bacteria bioassays are introduced to detect soil pollutants. One method is based on recombinant luminescent bacteria obtained with a gene-modification technique. This method can realize specific detection and enhance sensitivity, but it still cannot achieve undisturbed soil detection. The other method involves using magnetic nanoparticle (MNP)-based biosensors made from luminescent bacteria and MNPs. It can realize the accurate detection of the biological toxicity of the combined pollutants in soil without disturbing the soil’s integrity. This study shows that MNP-based biosensors have good application prospects in soil pollution detection, but the mechanism behind their utility still needs to be investigated to realize their popularization and application.

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Advances in bacterial whole-cell biosensors for the detection of bioavailable mercury: A review

Chen¹,

Liu

Xiang

et al. 2023

Science of The Total Environment

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Application of internal standard method in recombinant luminescent bacteria test

Cited by 5 publications

References 34 publications

Recent advances in the development and applications of luminescent bacteria–based biosensors

Recent advances in the development and applications of luminescent bacteria–based biosensors

Application of Luminescent Bacteria Bioassay in the Detection of Pollutants in Soil

Advances in bacterial whole-cell biosensors for the detection of bioavailable mercury: A review

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