Application of intermittent ball milling to enzymatic hydrolysis for efficient conversion of lignocellulosic biomass into glucose

Wu, Yingji; Ge, Shengbo; Xia, Changlei; Mei, Changtong; Kim, Ki‐Hyun; Cai, Liping; Smith, Lee M.; Lee, Jechan

doi:10.1016/j.rser.2020.110442

Cited by 61 publications

(19 citation statements)

References 39 publications

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“…One promising sustainable feedstock is (lignocellulosic) biomass, in which polymeric sugar chains, e.g., cellulose and hemicellulose, and lignin can be converted into a great variety of chemicals [3]. This can be done either by hydrolysis [4][5][6][7], fermentation [8][9][10], or combinations thereof (enzymatic hydrolysis) [11,12]. Typical hydrolysis products [4,7,13] include formic acid, levulinic acid, and furfural, formed via sugars and 5-hydroxymethylfurfural, while products from fermentation can be acetic acid [14,15], propionic acid [14,15], butyric acid [14,15], and L-lactic acid [14][15][16], but valeric acid [17], caproic acid [17], succinic acid [16,18], itaconic acid [16], mandelic acid [16], and alcohols [15] are also known to be produced.…”

Section: Introductionmentioning

confidence: 99%

Hydrophobic Deep Eutectic Solvents for the Recovery of Bio-Based Chemicals: Solid–Liquid Equilibria and Liquid–Liquid Extraction

et al. 2021

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The solid–liquid equilibrium (SLE) behavior and liquid–liquid extraction (LLX) abilities of deep eutectic solvents (DESs) containing (a) thymol and L-menthol, and (b) trioctylphosphine oxide (TOPO) and L-menthol were evaluated. The distribution coefficients (KD) were determined for the solutes relevant for two biorefinery cases, including formic acid, levulinic acid, furfural, acetic acid, propionic acid, butyric acid, and L-lactic acid. Overall, for both cases, an increasing KD was observed for both DESs for acids increasing in size and thus hydrophobicity. Furfural, being the most hydrophobic, was seen to extract the highest KD (for DES (a) 14.2 ± 2.2 and (b) 4.1 ± 0.3), and the KD of lactic acid was small, independent of the DESs (DES (a) 0.5 ± 0.07 and DES (b) 0.4 ± 0.05). The KD of the acids for the TOPO and L-menthol DES were in similar ranges as for traditional TOPO-containing composite solvents, while for the thymol/L-menthol DES, in the absence of the Lewis base functionality, a smaller KD was observed. The selectivity of formic acid and levulinic acid separation was different for the two DESs investigated because of the acid–base interaction of the phosphine group. The thymol and L-menthol DES was selective towards levulinic acid (Sij = 9.3 ± 0.10, and the TOPO and L-menthol DES was selective towards FA (Sij = 2.1 ± 0.28).

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Section: Introductionmentioning

confidence: 99%

Hydrophobic Deep Eutectic Solvents for the Recovery of Bio-Based Chemicals: Solid–Liquid Equilibria and Liquid–Liquid Extraction

et al. 2021

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“…Cellulose is a chain structure of large molecules and the crystallinity has a great influence in the field of fiber materials, such as mechanical properties, optical properties, thermal properties, etc. [1][2][3]. Nowadays, there are many methods to test the crystallinity of fibers, such as XRD and FT-IR, which detect the absorption peak characteristics of cellulose samples in a certain band [4][5][6].…”

Section: Introductionmentioning

confidence: 99%

Novel Terahertz Spectroscopy Technology for Crystallinity and Crystal Structure Analysis of Cellulose

et al. 2020

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Crystallinity is an essential indicator for evaluating the quality of fiber materials. Terahertz spectroscopy technology has excellent penetrability, no harmful substances, and commendable detection capability of absorption characteristics. The terahertz spectroscopy technology has great application potential in the field of fiber material research, especially for the characterization of the crystallinity of cellulose. In this work, the absorption peak of wood cellulose, microcrystalline cellulose, wood nano cellulose, and cotton nano cellulose were probed in the terahertz band to calculate the crystallinity, and the result compared with XRD and FT-IR analysis. The vibration model of cellulose molecular motion was obtained by density functional theory. The results showed that the average length of wood cellulose (WC) single fiber was 300 μm. The microcrystalline cellulose (MCC) was bar-like, and the average length was 20 μm. The cotton cellulose nanofiber (C-CNF) was a single fibrous substance with a length of 50 μm, while the wood cellulose nanofiber (W-CNF) was with a length of 250 μm. The crystallinity of cellulose samples in THz was calculated as follows: 73% for WC, 78% for MCC, 85% for W-CNF, and 90% for C-CNF. The crystallinity values were obtained by the three methods which were different to some extent. The absorption peak of the terahertz spectra was most obvious when the samples thickness was 1 mm and mixed mass ratio of the polyethylene and cellulose was 1:1. The degree of crystallinity was proportional to the terahertz absorption coefficients of cellulose, the five-movement models of cellulose molecules corresponded to the five absorption peak positions of cellulose.

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“…The results showed that following ball-milling pretreatment under the same substrate concentration conditions, chitosan without acid dissolution could also generate a large amount of products, overcoming the obstacle of acid pretreatment for substrates (Y. Wu et al, 2021).…”

Section: Enzymatic Hydrolysis Of Bmpcmentioning

confidence: 99%

Heterogenously‐expressed chitosanase combining a green ball milling method for enzymatic degradation

Sun

Guo

et al. 2022

Food Bioengineering

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Chitooligosaccharides (COSs) and D-glucosamine (GlcN), the most valuable biomolecules due to their various physiological functions, can be available inexpensively from chitosan by bio-enzymatic degradation, in which chitosanases play a key role. On the other hand, pretreatment of chitosan is the necessary procedure in the bioproduction by enzymatic hydrolysis. Therefore, the green and efficient pretreatment strategy as well as superior enzyme activity are the most critical aspects in scaled up production. This study investigated environmentally friendly and efficient enzymatic hydrolysis of powdery chitosan (PC) pretreated by ball-milling. First, heterologous expression yielded OUC-CsnPT, a glycoside hydrolase family 46 chitosanase with a predominant specific activity of 5346.56 U/mg at pH 6.0°C and 45°C. The endo-type chitosanase OUC-CsnPT hydrolyzed chitosan to produce GlcN and chitobiose [(GlcN) 2 ]. Then, ball-milling was employed to pretreat PC to promote chitosanase OUC-CsnPT hydrolysis, and the effectiveness was characterized by scanning electron microscope, X-ray diffraction, and FTIR-attenuated total reflection. Through the development of the novel route, the yield of COSs and GlcN for ball-milling powdery chitosan increased by 10.8-folds with a concentration of 14.5 μmol/ml. Hence, this technique presents a promising strategy suited to be a straightforward and environmentally friendly option for oligosaccharides production, broadening the theoretical basis for PC biodegradation.

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Application of intermittent ball milling to enzymatic hydrolysis for efficient conversion of lignocellulosic biomass into glucose

Cited by 61 publications

References 39 publications

Hydrophobic Deep Eutectic Solvents for the Recovery of Bio-Based Chemicals: Solid–Liquid Equilibria and Liquid–Liquid Extraction

Hydrophobic Deep Eutectic Solvents for the Recovery of Bio-Based Chemicals: Solid–Liquid Equilibria and Liquid–Liquid Extraction

Novel Terahertz Spectroscopy Technology for Crystallinity and Crystal Structure Analysis of Cellulose

Heterogenously‐expressed chitosanase combining a green ball milling method for enzymatic degradation

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