Application of injectable silk fibroin/graphene oxide hydrogel combined with bone marrow mesenchymal stem cells in bone tissue engineering

Wang, Lu; Lü, Rui; Hou, Jiaxin; Nan, Xiaoru; Xia, Yijing; Guo, Yanqin; Meng, Kejing; Xu, Changzhen; Wang, Xiangyu; Zhao, Bin

doi:10.1016/j.colsurfa.2020.125318

Cited by 43 publications

(32 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Among them, fibroin and cellulose are optimal candidates: the first has, in principle, maximum physico-chemical compatibility with silk fibers, while the second, in particular in the form of cellulose nanofibrils (CNFs) and nanocrystals (CNC), exhibits ideal mechanical properties [21][22][23]. Not surprisingly, fibroin-silk and fibroin-nanocellulose composites are of great interest in the fields of biomaterials and materials engineering [24][25][26][27][28][29][30], but, to the best of our knowledge, their formulation and use in the restoration of aged silk textiles is still almost unexplored. Hao et al have evaluated carboxymethyl chitosan (CMC)-silk fibroin composites for the reinforcement of aged silk, and found that CMC significantly enhances the mechanical improvement provided by fibroin on degraded silk fibers, likely thanks to interactions taking place between surface groups in chitosan and fibroin, leading to the formation of semi-interpenetrating structures [31].…”

Section: Introductionmentioning

confidence: 99%

Hybrid fibroin-nanocellulose composites for the consolidation of aged and historical silk

Cianci

Chelazzi

Poggi

et al. 2022

Colloids and Surfaces A: Physicochemical and Engineering Aspect

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 99%

Hybrid fibroin-nanocellulose composites for the consolidation of aged and historical silk

Cianci

Chelazzi

Poggi

et al. 2022

Colloids and Surfaces A: Physicochemical and Engineering Aspect

View full text Add to dashboard Cite

“…The most representative DBM peaks were identified at 1600 and 1500 cm −1 , which are attributed to vibrations of the amide groups, of collagen and other proteins, respectively [43]. The band at 1032 cm −1 and the beginning of the band at 550 cm −1 are associated with the bending mode caused by vibration of phosphate group PO 4 3− , confirming the presence of calcium phosphates, a mineral component of bone that remains as a residual mineral component after the demineralization of DBM [44]. The black spectrum corresponding to PVA shows the characteristic bands of polyvinyl alcohol.…”

Section: Physicochemical Characterizationmentioning

confidence: 97%

“…In addition, IBSs facilitate the filling of any irregularly shaped defect, which allows for direct contact between the injected material and the surface of the bone tissue to be treated [3]. Injectable hydrogels have attracted attention, especially because their structures are similar to those of extracellular matrixes [4] and their capacity to promote the adhesion, proliferation, differentiation, and migration of stem cells [5]. In recent years, various injectable hydrogels with good formability and three-dimensional structures have been studied for potential applications in bone tissue engineering [6].…”

Section: Introductionmentioning

confidence: 99%

Formulation and Characterization of a New Injectable Bone Substitute Composed PVA/Borax/CaCO3 and Demineralized Bone Matrix

Medrano-David

Lopera

Londoño

et al. 2021

JFB

View full text Add to dashboard Cite

The occurrence of bone-related disorders and diseases has dramatically increased in recent years around the world. Demineralized bone matrix (DBM) has been widely used as a bone implant due to its osteoinduction and bioactivity. However, the use of DBM is limited because it is a particulate material, which makes it difficult to manipulate and implant with precision. In addition, these particles are susceptible to migration to other sites. To address this situation, DBM is commonly incorporated into a variety of carriers. An injectable scaffold has advantages over bone grafts or preformed scaffolds, such as the ability to flow and fill a bone defect. The aim of this research was to develop a DBM carrier with such viscoelastic properties in order to obtain an injectable bone substitute (IBS). The developed DBM carrier consisted of a PVA/glycerol network cross-linked with borax and reinforced with CaCO3 as a pH neutralizer, porosity generator, and source of Ca. The physicochemical properties were determined by an injectability test, FTIR, SEM, and TGA. Porosity, degradation, bioactivity, possible cytotoxic effect, and proliferation in osteoblasts were also determined. The results showed that the developed material has great potential to be used in bone tissue regeneration.

show abstract

“…More specifically, the samples did not present any significant difference until the 3rd day, while after the 7th and 14th day, a high proliferation rate was detected. It should be noted that the maximum absorbance at 450 nm corresponded to the composite SF/0.05%GO [97,98].…”

Section: Graphene and Graphene Oxidementioning

confidence: 99%

Impact of Graphene Derivatives as Artificial Extracellular Matrices on Mesenchymal Stem Cells

et al. 2022

View full text Add to dashboard Cite

Thanks to stem cells’ capability to differentiate into multiple cell types, damaged human tissues and organs can be rapidly well-repaired. Therefore, their applicability in the emerging field of regenerative medicine can be further expanded, serving as a promising multifunctional tool for tissue engineering, treatments for various diseases, and other biomedical applications as well. However, the differentiation and survival of the stem cells into specific lineages is crucial to be exclusively controlled. In this frame, growth factors and chemical agents are utilized to stimulate and adjust proliferation and differentiation of the stem cells, although challenges related with degradation, side effects, and high cost should be overcome. Owing to their unique physicochemical and biological properties, graphene-based nanomaterials have been widely used as scaffolds to manipulate stem cell growth and differentiation potential. Herein, we provide the most recent research progress in mesenchymal stem cells (MSCs) growth, differentiation and function utilizing graphene derivatives as extracellular scaffolds. The interaction of graphene derivatives in human and rat MSCs has been also evaluated. Graphene-based nanomaterials are biocompatible, exhibiting a great potential applicability in stem-cell-mediated regenerative medicine as they may promote the behaviour control of the stem cells. Finally, the challenges, prospects and future trends in the field are discussed.

show abstract

Application of injectable silk fibroin/graphene oxide hydrogel combined with bone marrow mesenchymal stem cells in bone tissue engineering

Cited by 43 publications

References 41 publications

Hybrid fibroin-nanocellulose composites for the consolidation of aged and historical silk

Hybrid fibroin-nanocellulose composites for the consolidation of aged and historical silk

Formulation and Characterization of a New Injectable Bone Substitute Composed PVA/Borax/CaCO3 and Demineralized Bone Matrix

Impact of Graphene Derivatives as Artificial Extracellular Matrices on Mesenchymal Stem Cells

Contact Info

Product

Resources

About