Application of in Vivo Microdialysis to the Study of Cholinergic Systems

Day, Jamie C.; Kornecook, Tom J.; Quirion, Rémi

doi:10.1006/meth.2000.1103

Cited by 46 publications

(45 citation statements)

References 225 publications

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“…This is comparable to the level reported in the rat dorsal hippocampus (Day et al, 2001). Interestingly, the basal level of ACh in the d5 knockout mouse (2.8 nM) was significantly lower than in heterozygous (4.0 nM) and wild-type (4.4 nM) mice.…”

Section: Discussionsupporting

confidence: 89%

“…The no net flux microdialysis technique is used to determine the in vivo extracellular concentration of a neurotransmitter (Day et al, 2001;Justice, 1993). Another group of animals was perfused with the same cerebrospinal fluid-like solution used in the former experiment without neostigmine bromine but containing a known concentration of ACh (2. …”

Section: No Net Flux Methodsmentioning

confidence: 99%

“…The microdialysis probe was implanted in the dorsal hippocampus according to procedures described by our group for rats with minor modifications (Day et al, 2001;Hersi et al, 1995a;Kitaichi et al, 1999). Male mice, 4-month old, were anesthetized using a mix of ketamine (100 mg/kg), zylazine (20 mg/kg), and acepromazine (10 mg/kg) administered intramuscularly.…”

Section: Probe Implantationmentioning

confidence: 99%

“…ACh content in dialysate fractions was determined using HPLC separation, postcolumn enzymatic reaction, and electrochemical detection (Day et al, 2001). ACh was separated from other molecules in the dialysate on a reverse phase column (75 Â 2.1 mm) pretreated with sodium lauryl sulfate.…”

Section: Ach Measurementmentioning

confidence: 99%

“…Dopamine (DA) exerts a modulatory role on acetylcholine (ACh) release in the hippocampus (Day et al, 2001;Hersi et al, 1995a, b). Interactions between these two neurotransmitter systems play key roles in learning and memory (Levin et al, 1990) and these systems, particularly the cholinergic innervation, are severely affected in Alzheimer's Disease (Araujo et al, 1988;Auld et al, 2002;Davies and Maloney, 1976;Perry et al, 1992).…”

Section: Introductionmentioning

confidence: 99%

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Reduction in Acetylcholine Release in the Hippocampus of Dopamine D5 Receptor-Deficient Mice

Laplante

Sibley

Quirion

2004

Neuropsychopharmacol

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Activation of the dopamine D 1 -like receptor stimulates acetylcholine (ACh) release in the hippocampus, apparently through the molecularly defined d5 receptor. In the present study, we used a transgenic mouse completely deprived of functional d5 receptor (d5À/À) to confirm the role and elucidate the possible function of the d5 receptor subtype on hippocampal cholinergic neurotransmission. ACh release was measured using in vivo microdialysis in the mouse dorsal hippocampus of 4 months old homozygous (d5À/À), heterozygous (d5 þ /À), and the wild-type (d5 þ / þ ) littermates. Using the no net flux technique, a significant reduction in basal hippocampal ACh level was found in the d5À/À compared to d5 þ /À and d5 þ / þ mice. Moreover, the administration of SKF 38393, a D 1 -like receptor agonist, systemically (2.0 and 10.0 mg/kg ip), or locally through the dialysis probe (10 and 50 mM), produced a dose-dependent enhancement of ACh release in the d5 þ / þ , a moderate stimulation in the d5 þ /À but had no effect in the d5À/À mice. Quantitative receptor autoradiography revealed significant increases in M 1 -like but not in M 2 -like muscarinic receptor binding sites in the hippocampal formation. These results confirm and extend the role of the d5 receptor in the modulation of hippocampal ACh release and provide evidence for long-term alteration of hippocampal cholinergic neurotransmission resulting from the absence of the d5 receptors including chronically reduced ACh release and change in M 1 -like receptor levels.

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Section: Discussionsupporting

confidence: 89%

Section: No Net Flux Methodsmentioning

confidence: 99%

Section: Probe Implantationmentioning

confidence: 99%

Section: Ach Measurementmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Reduction in Acetylcholine Release in the Hippocampus of Dopamine D5 Receptor-Deficient Mice

Laplante

Sibley

Quirion

2004

Neuropsychopharmacol

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Triarylpyridinium-Functionalized Terpyridyl Ligand for Photosensitized Supramolecular Architectures: Intercomponent Coupling and Photoinduced Processes Triarylpyridinium-Functionalized Terpyridyl Ligands for Photosensitized Supramolecular Architectures, Part 2. For Part 1 see reference 9.

et al. 2002

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Quantification of acetylcholine, an essential neurotransmitter, in brain microdialysis samples by liquid chromatography mass spectrometry

Nirogi

Mudigonda

Kandikere

et al. 2009

Biomedical Chromatography

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Chemical neurotransmission has been the subject of intensive investigations in recent years. Acetylcholine is an essential neurotransmitter in the central nervous system as it has an effect on alertness, memory and learning. Enzymatic hydrolysis of acetylcholine in the synaptic cleft is fast and quickly metabolizes to choline and acetate by acetylcholinesterase. Hence the concentration in the extracellular fluid of the brain is low (0.1-6 nm). Techniques such as microdialysis are routinely employed to measure acetylcholine levels in living brain systems and the microdialysis sample volumes are usually less than 50 microL. In order to develop medicine for the diseases associated with cognitive dysfunction like mild cognitive impairment, Alzheimer's disease, schizophrenia and Parkinson's disease, or to study the mechanism of the illness, it is important to measure the concentration of acetylcholine in the extracellular fluid of the brain. Recently considerable attention has been focused on the development of chromatographic-mass spectrometric techniques to provide more sensitive and accurate quantification of acetylcholine collected from in-vivo brain microdialysis experiments. This review will provide a brief overview of acetylcholine biosynthesis, microdialysis technique and liquid chromatography mass spectrometry, which is being used to quantitate extracellular levels of acetylcholine.

show abstract

Application of in Vivo Microdialysis to the Study of Cholinergic Systems

Cited by 46 publications

References 225 publications

Reduction in Acetylcholine Release in the Hippocampus of Dopamine D5 Receptor-Deficient Mice

Reduction in Acetylcholine Release in the Hippocampus of Dopamine D5 Receptor-Deficient Mice

Triarylpyridinium-Functionalized Terpyridyl Ligand for Photosensitized Supramolecular Architectures: Intercomponent Coupling and Photoinduced Processes Triarylpyridinium-Functionalized Terpyridyl Ligands for Photosensitized Supramolecular Architectures, Part 2. For Part 1 see reference 9.

Quantification of acetylcholine, an essential neurotransmitter, in brain microdialysis samples by liquid chromatography mass spectrometry

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