Application of in situ Solid-Phase Microextraction on Mediterranean Sponges for Untargeted Exometabolome Screening and Environmental Monitoring

Bojko, Barbara; Onat, Bora; Boyacı, Ezel; Psillakis, Elefteria; Dailianis, Thanos; Pawliszyn, Janusz

doi:10.3389/fmars.2019.00632

Cited by 20 publications

(14 citation statements)

References 87 publications

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“…This suggests that the previous reports on AHLs from sponges underestimated the variety of signals found in these invertebrates. The presence of these long-chain AHLs in marine sponges may relate to their better stability at the high pH of seawater [20,40]. We report here the presence of putative C19-AHL and OC19-AHL in the AHL-enriched fraction of sponge, which, to the best of our knowledge, have never been reported from sponges.…”

Section: Identification Of Ahls In Sarcotragus Spinosulus Crude Extractsmentioning

confidence: 51%

Identification of Quorum Sensing Activators and Inhibitors in The Marine Sponge Sarcotragus spinosulus

et al. 2020

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Marine sponges, a well-documented prolific source of natural products, harbor highly diverse microbial communities. Their extracts were previously shown to contain quorum sensing (QS) signal molecules of the N-acyl homoserine lactone (AHL) type, known to orchestrate bacterial gene regulation. Some bacteria and eukaryotic organisms are known to produce molecules that can interfere with QS signaling, thus affecting microbial genetic regulation and function. In the present study, we established the production of both QS signal molecules as well as QS inhibitory (QSI) molecules in the sponge species Sarcotragus spinosulus. A total of eighteen saturated acyl chain AHLs were identified along with six unsaturated acyl chain AHLs. Bioassay-guided purification led to the isolation of two brominated metabolites with QSI activity. The structures of these compounds were elucidated by comparative spectral analysis of 1HNMR and HR-MS data and were identified as 3-bromo-4-methoxyphenethylamine (1) and 5,6-dibromo-N,N-dimethyltryptamine (2). The QSI activity of compounds 1 and 2 was evaluated using reporter gene assays for long- and short-chain AHL signals (Escherichia coli pSB1075 and E. coli pSB401, respectively). QSI activity was further confirmed by measuring dose-dependent inhibition of proteolytic activity and pyocyanin production in Pseudomonas aeruginosa PAO1. The obtained results show the coexistence of QS and QSI in S. spinosulus, a complex signal network that may mediate the orchestrated function of the microbiome within the sponge holobiont.

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Section: Identification Of Ahls In Sarcotragus Spinosulus Crude Extractsmentioning

confidence: 51%

Identification of Quorum Sensing Activators and Inhibitors in The Marine Sponge Sarcotragus spinosulus

et al. 2020

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“…Thus, the utilization of different extraction methods on both sponge tissue and enriched microbial fraction, enabled a more exhaustive description of the wide variety of AHLs that can be found in S. spinosulus, identifying a wide range of AHLs including unusual long acyl chain AHLs (Table 3). The presence of these long chain AHLs in marine sponges may relate to their better stability at the high pH typical of seawater [24,44]. We report here the presence of putative C19-AHL and OXO-C19-AHL in the AHL-enriched fraction of sponge, that, to the best of our knowledge have never been reported from sponges.…”

Section: Identification Of Ahls In Sarcotragus Spinosulus Crude Extractsmentioning

confidence: 55%

Co-existence of Quorum Sensing and Quorum Sensing Inhibitory Compounds in Marine Sponge <em>Sarcotragus spinosulus</em>

Saurav¹,

Borbone²,

Burgsdorf³

et al. 2019

Preprint

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Marine sponges, a well documented prolific source of natural products, harbors numerous microbial communities believed to possess N-acyl homoserine lactones (AHLs) mediated Quorum sensing (QS) as one of the mechanisms of interaction. Bacteria and eukaryotic organisms are known to produce molecules that can interfere with QS signaling, thus affecting microbial genetic regulation and function. In the present study, we established the potential for production of both QS signal molecules as well as QS interfering molecules (QSI) in the same sponge species Sarcotragus spinosulus. A total of eighteen saturated acyl chain AHLs were identified along with six putative unsaturated acyl chain AHLs. Bioassay guided purification led to the isolation of two brominated metabolites with QS-interfering activity. The structures of these compounds were elucidated by comparative spectral analysis of 1HNMR and HR-MS data and was identified as 3-Br-N-methyltyramine (1) and 5,6-dibromo-N,N-dimethyltryptamine (2). The QSI activity of compounds 1 and 2 were evaluated using reporter gene assays for long- and short-chain signals (E. coli pSB1075 and E. coli pSB401) and was confirmed by measuring dose dependent inhibition of proteolytic activity and pyocyanin production in P. aeruginosa PAO1. The obtained results showed the co-existence of QS and QSI in S. spinosulus, a complex network which may mediate the orchestrated function of the microbiome within the sponge holobiont.

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“…After determining the species and compounds of interest, the next step is to compare the organism growth/metabolic engineering conditions, productivity and yields of the compound(s) of interest. Novel approaches such as the use of Solid-Phase Microextraction (SPME) have been recently demonstrated as a successful means of noninvasive untargeted metabolome screening of marine organisms (Bojko et al, 2019). This method comprises the use of specially coated probes that combine metabolite sampling and extraction in a single step, thus allowing in vivo metabolomic screening in the marine environment, while allowing both polar and non-polar metabolites to be extracted efficiently (Reyes-Garcés et al, 2018), thus emerging as a useful field tool to discover novel compounds of interest from complex marine holobionts.…”

Section: Methodology For Exploration Of Marine Bioresources Data Analmentioning

confidence: 99%

The Essentials of Marine Biotechnology

Barbier²,

et al. 2021

Self Cite

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Coastal countries have traditionally relied on the existing marine resources (e.g., fishing, food, transport, recreation, and tourism) as well as tried to support new economic endeavors (ocean energy, desalination for water supply, and seabed mining). Modern societies and lifestyle resulted in an increased demand for dietary diversity, better health and well-being, new biomedicines, natural cosmeceuticals, environmental conservation, and sustainable energy sources. These societal needs stimulated the interest of researchers on the diverse and underexplored marine environments as promising and sustainable sources of biomolecules and biomass, and they are addressed by the emerging field of marine (blue) biotechnology. Blue biotechnology provides opportunities for a wide range of initiatives of commercial interest for the pharmaceutical, biomedical, cosmetic, nutraceutical, food, feed, agricultural, and related industries. This article synthesizes the essence, opportunities, responsibilities, and challenges encountered in marine biotechnology and outlines the attainment and valorization of directly derived or bio-inspired products from marine organisms. First, the concept of bioeconomy is introduced. Then, the diversity of marine bioresources including an overview of the most prominent marine organisms and their potential for biotechnological uses are described. This is followed by introducing methodologies for exploration of these resources and the main use case scenarios in energy, food and feed, agronomy, bioremediation and climate change, cosmeceuticals, bio-inspired materials, healthcare, and well-being sectors. The key aspects in the fields of legislation and funding are provided, with the emphasis on the importance of communication and stakeholder engagement at all levels of biotechnology development. Finally, vital overarching concepts, such as the quadruple helix and Responsible Research and Innovation principle are highlighted as important to follow within the marine biotechnology field. The authors of this review are collaborating under the European Commission-funded Cooperation in Science and Technology (COST) Action Ocean4Biotech – European transdisciplinary networking platform for marine biotechnology and focus the study on the European state of affairs.

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Application of in situ Solid-Phase Microextraction on Mediterranean Sponges for Untargeted Exometabolome Screening and Environmental Monitoring

Cited by 20 publications

References 87 publications

Identification of Quorum Sensing Activators and Inhibitors in The Marine Sponge Sarcotragus spinosulus

Identification of Quorum Sensing Activators and Inhibitors in The Marine Sponge Sarcotragus spinosulus

Co-existence of Quorum Sensing and Quorum Sensing Inhibitory Compounds in Marine Sponge <em>Sarcotragus spinosulus</em>

The Essentials of Marine Biotechnology

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