Application of immunoassay for detection of Helicobacter pylori antigens in the dental plaque

Leszczyńska, Katarzyna; Namiot, DB; Namiot, Z; Leszczyńska, JK; Jakoniuk, P; Kemonä, A

doi:10.2478/v10039-009-0050-3

Cited by 12 publications

(12 citation statements)

References 12 publications

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“…Using this method for detection of H. pylori in dental plaque, 2 studies [26, 27] reported a high prevalence (>65%) of the microorganism in dental plaque while other 2 studies [28, 29] reported a very low prevalence (0 and 11%).…”

Section: Methodsmentioning

confidence: 99%

“…Out of the 5 studies reviewed which employed microbial culture, the rates of prevalence reported were 1%, 1.4%, 6.9%, 14.6%, and 18%, respectively [16, 31–33]. In the 2 studies reviewed which used immunoassays for detection of pathogen in dental plaque samples, the prevalence rates reported were 23.6% and 47.6% [27]. Among patients with gastrointestinal colonization by H. pylori , the prevalence of coinfection in dental plaque was reported to be in the range of 25.2% to 100%.…”

Section: Methodsmentioning

confidence: 99%

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Oral Cavity as an Extragastric Reservoir of Helicobacter pylori

Sayed

Anand

Kamath

et al. 2014

ISRN Gastroenterology

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Background. Several studies were reported on the prevalence, and relationship between the existence of Helicobacter pylori (H. pylori) in oral cavity and in stomach of patients. The purpose of this study was to systematically review the existing literature on the presence of H. pylori in the oral cavity and its link to gastric infection, the existence of coinfection, and the impact of anti-H. pylori therapy on the dental plaque and vice versa. Method. Two authors independently searched the Medline, EMBASE, Cochrane Library, Web of Science, Google Scholar, and Scopus databases for relevant studies. The articles were analyzed critically and all qualified studies were included. The search was carried out by using a combined text and the MeSH search strategies: using the key words Helicobacter, Helicobacter pylori, and H. pylori in combination with dental plaque, periodontitis, and oral hygiene. Results. The data was presented in 8 tables and each topic separately discussed. Conclusion. Based on the systematic review of the available literature on H. pylori infection and its presence in the oral cavity, it can be concluded that dental plaque can act as a reservoir, and proper oral hygiene maintenance is essential to prevent reinfection. Due to the diversified methods and population groups involved in the available literature, no concrete evidence can be laid down. Further studies are necessary to establish the role of H. pylori in the oral cavity and its eradication on preventing the gastroduodenal infection.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Oral Cavity as an Extragastric Reservoir of Helicobacter pylori

Sayed

Anand

Kamath

et al. 2014

ISRN Gastroenterology

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“…This procedure increases the incidence of H. pylori more than twice. 31 Using this method, a high prevalence of these microorganisms in dental plaque was reported, i.e., 58.7% 32 and 65.6%. 30 It was suggested that plaque reduction and oral health had no effect on the amount of antigens of these bacteria.…”

Section: Epidemiology Of Oral H Pylori Infectionsmentioning

confidence: 98%

“…14 Namiot et al, in oral H. pylori detection, used immunoassays intended routinely for fecal antigen detection. [30][31][32] The authors suggest that these tests can be successfully used to detect H. pylori in the oral cavity after appropriate sample preparation (preincubation in a microaerophilic atmosphere). This procedure increases the incidence of H. pylori more than twice.…”

Section: Epidemiology Of Oral H Pylori Infectionsmentioning

confidence: 99%

Oral Helicobacter pylori: Interactions with host and microbial flora of the oral cavity

Krzyżek

Gościniak

2018

Dent. Med. Probl.

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The role of the oral cavity as a reservoir for Helicobacter pylori is still a controversial issue. There is a great number of articles indicating the presence of this pathogen in the oral cavity, but discrepancies among techniques for H. pylori detection and the variations in the patients tested often make it difficult to formulate a final verdict. Outer membrane proteins (OMPs) are one of the most important factors determining colonization of H. pylori in the oral cavity. Among them, the key role is attributed to BabA, SabA and NapA, all of which promote adherence and retention within this area. The oral cavity is characterized by the co-existence of numerous microorganisms which may potentially affect the physiology and morphology of H. pylori. The presence of coccoid-stimulating factors and relatively low levels of AI-2 in the early-to mid-stages of supragingival plaque allow dental H. pylori to colonize this niche as nonculturable spherical forms. On the other hand, subgingival plaque characterized by high numbers of periopathogens, capable of synthesizing high concentrations of AI-2, may favor the presence of mixed populations of spiral and coccoid H. pylori forms. This review article provides an up-to-date knowledge about the ability of oral H. pylori to interact both with the host and the local microflora of the oral cavity.

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“…'ye karşı etkili olduğu ve böylelikle oral mikrobiyal hastalıkların, diş çürümesi ve periyodontit tedavisinde kullanıla-bildiği saptanmıştır (21) . Son çalışmalar CSA-13'ün, klaritromisin yada metronidazol dirençli Helicobacter pylori infeksiyonlarının tedavisinde de kullanılabildiğini göstermektedir (25) . Ayrıca yapılan çalışmalar sonucunda CSA-13'ün lineer peptid olan LL-37 ve WLBU2'den farklı olarak, düşük pH'da pepsin varlığında preinkübasyona konulması gibi yaratılan uygunsuz koşullarda bile güçlü bakterisidal etki gösterebildiği anlaşılmıştır.…”

Section: -Csa'ların In Vitro Etki Spektrumuunclassified