Application of Deconica castanella ligninolytic enzymatic system in the degradation of hexachlorobenzene in soil

Abstract

Hexachlorobenzene (HCB) is a pollutant still found in the environment despite being widely banned. Considering that basidiomycetes are useful to degrade a variety of organochlorinated pollutants, we therefore report the influence of HCB on the ligninolytic enzymatic system of Deconica castanella. The inoculum was prepared with sugarcane bagasse and soybean flour and was added in soil with and without HCB (2000 mg kg soil −1 ), 5% emulsion containing soybean oil and Tween 20 at proportion 9:1, v:v; with 70% moisture at 25 • C. Fungal biomass was quantified by widely acknowledged growth biomarker ergosterol. The extraction of the enzymatic complex was performed and laccase, Mn-dependent peroxidase (MnP), and lignin peroxidase (LiP) activities were determined. Furthermore, HCB and its metabolites were quantified by gas chromatography and chlorides by potentiometric titration. Results evidenced that HCB did not interfere in fungal growth, though the only detected enzymatic activity was laccase. MnP and Lip were not detected during D. castanella growth in soil. The peak of laccase enzymatic activity occurred in the presence of HCB. In addition, the laccase exhibited thermostability. Therefore, we hereby shed light on the role of laccase in the degradation of HCB by an efficient low-cost and environmentally safe detoxification mechanism.

Ergosterol and Its Metabolites Induce Ligninolytic Activity in the Lignin-Degrading Fungus Phanerochaete sordida YK-624

“…In biotechnological research, ergosterol is commonly utilized as a growth biomarker to quantify fungal biomass in solid substrates, exhibiting a positive correlation [ 28 ]. Interestingly, ergosterol has been identified as an indicator for evaluating the growth of Lentinus crinitus during soil bioremediation processes.…”

Molecularly Imprinted Polymer-Based Electrochemical Sensing in Protein Detection

“…In practical applications, this electrochemical transduction process offers numerous advantages. It enables real-time and rapid detection of target analytes with high sensitivity and selectivity, making it ideal for applications in healthcare [28][29][30], environmental monitoring [31][32][33][34][35][36][37][38][39][40][41], food safety [42][43][44][45][46][47][48][49], and pharmaceutical research [50][51][52][53][54][55]. This field's continuous research and development strives to further optimise the electrochemical transduction process, aiming for enhanced performance, increased versatility and expanded applications across a broad spectrum of analytical domains.…”