Application of hydrocyclone for separation of <i>Pichia pastoris</i> produced r-HBsAg from fermentation culture: impact of concentration and pressure on hydrocyclone performance

Mazraeno, Masoumeh Seyfi; Fazlali, Alireza; Hosseini, Seyed Nezamedin

doi:10.1080/10826068.2019.1621891

Cited by 4 publications

(2 citation statements)

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“…Numerous investigations were done on the effect of feed concentration on hydrocyclone performance [21,48,49]. It was reported that the separation performance decreased with increasing feed concentration [49].…”

Section: Performance Of the Minihydrocyclonesmentioning

confidence: 99%

“…Previous research introduced a new application for minihydrocyclones in the pharmaceutical industry [21], i.e., Pichia pastoris separation from fermentation culture in hepatitis B vaccine production plant was studied experimentally. However, the mentioned research or others did not involve computational simulation for particle separation in the vaccine industry, and hence they cannot predict reliable results without experimental implementation.…”

Section: Introductionmentioning

confidence: 99%

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Numerical and Experimental Biomass Separation from Fermentation Process by Minihydrocyclones

et al. 2020

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Biomass particle separation is challenging in the pharmaceutical industry because of the smallness and lightness of such particles. Centrifugation is applied conventionally for batch separation. However, imposing multiple wash steps in order to eliminate culture residues and the high risk of extrinsic contamination hamper batch techniques. In this research, minihydrocyclones were introduced to provide a better solution for the mentioned limitations in continuous processes. Numerical separation of methylotrophic yeast from fermentation broth was carried out in three hydrocyclones with different lengths, and the most efficient one was examined experimentally. Three various feed flow rates and seven feed concentrations were evaluated. The concentration of biomass suspension in the product was raised to 20 %.

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