Application of Human Adipose Stem Cell (hADSC) in Collagen Gel on Rat Deep Dermal Burn

Abstract

Human adipose derived stem cells (hADSC) is expected to be an efficient solution for healing deep-dermal burns that help in wound closure. This study used 20 male Sprague dawley rats. Each rat received three wounds with different treatments (control, hADSC in collagen gel and collagen gel). Wound closure was observed at day 7th, 14th, 21th and 28th (sacrificed day) macroscopically, wound areas were measured and collagen densities were evaluated. Reduction of wound area showed significant difference between hADSC in collagen gel group and control group (p 0.001) and between hADSC in collagen gel group and collagen gel group. The hADSC in collagen gel group had a higher collagen density compared to control and collagen gel group. Application of hADSC in bovine collagen gel in rat deep-dermal burn models provided faster and better wound healing compared with control and bovine collagen gel group.

Emulgel based on fish skin collagen‐microalgae‐silver increased neovascularization and re‐epithelialization of full thickness burn in rats

“…Likewise, xenogeneic collagen‐based biomaterials have demonstrated a beneficial effect on wound healing. For instance, bovine collagen gels enhanced angiogenesis and collagen deposition in rat full thickness burns, resulting in complete re‐epithelialization after 28 days 68,70 . In addition, collagen and gelatin from tilapia have also shown attenuation of the inflammatory response, increased neovascularization, fibroblast proliferation, and re‐epithelialization in vivo 6,71 .…”

“…For instance, bovine collagen gels enhanced angiogenesis and collagen deposition in rat full thickness burns, resulting in complete re-epithelialization after 28 days. 68,70 Moreover, collagen and gelatin derived from tilapia reduced inflammation, increased neovascularization, fibroblast proliferation, and reepithelialization in vivo. 6,71 Xenogeneic collagen and gelatin also showed good biocompatibility and biodegradability in vivo.…”

“…For instance, bovine collagen gels enhanced angiogenesis and collagen deposition in rat full thickness burns, resulting in complete re-epithelialization after 28 days. 68,70 In addition, collagen and gelatin from tilapia have also shown attenuation of the inflammatory response, increased neovascularization, fibroblast proliferation, and re-epithelialization in vivo. 6,71 Good biocompatibility and biodegradation in vivo also were related to xenogeneic collagen and gelatin.…”

Efektivitas Adipose Derived Stem Cell Terhadap Penyembuhan Luka Bakar: Telaah Sistematis

“…3) Sekresi faktor pertumbuhan, ADSC dikenal karena kemampuannya untuk mengeluarkan berbagai faktor pertumbuhan, seperti HGF (hepatocyte growth factor) dan VEGF (vascular endothelial growth factor), yang dapat mempromosikan penyembuhan jaringan melalui aktivitas angiogenik dan anti-apoptosis (Dong et al, 2020;Junaidi et al, 2019;Ng et al, 2021;Yunaini et al, 2019) .…”

“…Studi ini juga menyoroti biokompatibilitas dan biodegradabilitas kolagen eksogen, menjadikannya bahan yang ideal untuk aplikasi biomedis, yang selanjutnya mendukung potensi hADSC dalam gel kolagen untuk penyembuhan luka bakar (Junaidi et al, 2019).…”