Application of HPCCC, UHPLC-PDA-ESI-MS 3 and HPLC-PDA methods for rapid, one-step preparative separation and quantification of rutin in Forsythia flowers

Kicel, Agnieszka; Owczarek, Aleksandra; Michel, Piotr; Skalicka-Woźniak, Krystyna; Kiss, Anna K.; Olszewska, Monika A.

doi:10.1016/j.indcrop.2015.06.019

Cited by 27 publications

(16 citation statements)

References 34 publications

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“…Phenolic acids, namely ellagic and gallic acid, have been linked to potential health benefits including antioxidant, anticarcinogenic, and anti-inflammatory activities and decrease of cardiovascular disease risk [6][7][8]. Indeed, flavonoids exert numerous biochemical and pharmacological activities, being associated with the increase resistance of blood vessels, treatment of chronic venous insufficiency and improvement of microvascular blood flow [9,10]. According to Morana et al, the inner shell can contain between 2.7% and 5.2% of phenolic compounds [11].…”

Section: Introductionmentioning

confidence: 99%

Green-Sustainable Recovery of Phenolic and Antioxidant Compounds from Industrial Chestnut Shells Using Ultrasound-Assisted Extraction: Optimization and Evaluation of Biological Activities In Vitro

et al. 2020

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Chestnut processing industry generates large amounts of by-products, including leaves, burs and shells that are a source of bioactive compounds. The purpose of this study was to establish an ultrasound-assisted extraction (UAE) of phenolic and antioxidant compounds from industrial chestnut shells. A central composite design (CCD) was conducted to analyze the effects of time (4–46 min) and temperature (34–76 °C) in the antioxidant activity (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH), and ferric reducing antioxidant power (FRAP)) and total phenolic compounds (TPC) of chestnut shells extracts. The optimal extraction conditions were obtained at 70 °C for 40 min. The optimal extract was characterized regarding phenolic profile, radical scavenging capacity, and effects on intestinal and dermal cell lines. The optimal extract revealed high amounts of ellagic acid (40.4 µg/mg dw), followed by caffeic acid derivative (15.4 µg/mg dw) and epigallocatechin (15.3 µg/mg dw). Indeed, the extract exhibited the highest scavenging efficiencies against NO● (IC50 = 0.1 µg/mL) and HOCl (IC50 = 0.7 µg/mL) and did not conducted to a decrease on HaCaT and HFF-1 viability up to 100 μg/mL. Oppositely, a decrease on Caco-2 and HT29-MTX viability was observed. This study suggests that UAE could be a sustainable option to valorize chestnut shells as raw material for different industries.

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Section: Introductionmentioning

confidence: 99%

Green-Sustainable Recovery of Phenolic and Antioxidant Compounds from Industrial Chestnut Shells Using Ultrasound-Assisted Extraction: Optimization and Evaluation of Biological Activities In Vitro

et al. 2020

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“…The electrochemical behavior of the as-prepared paste electrodes was investigated by cyclic voltammetry and electrochemical impedance spectroscopy employing Fe(CN) 6 3À/4À as the electrochemical probe. As shown in Fig.…”

Section: Electrochemical Characterization Of the Modied Electrodementioning

confidence: 99%

“…The effective surface area of the constructed electrodes was further examined by recording CVs of 1 mmol L À1 K 3 [Fe(CN) 6 ] at different potential scan rates. The calculations were performed according to the Randles-Servick equation: 48 I p ¼ 2.69 Â 10 5 AD 1/2 n 3/2 Cy 1/2 (1) where I p refers to the peak current (A), A is the effective surface area (cm 2 ), D is the diffusion coefficient of K 3 [Fe(CN) 6 ] in the solution (7.66 Â 10 À 6 cm 2 s À1 ) at 1 mol L À1 KCl, 49 n is the electron transfer number, C is the concentration of K 3 [Fe(CN) 6 ] (mol cm À3 ) and y is the scan rate (V s À1 ).…”

Section: Electrochemical Characterization Of the Modied Electrodementioning

confidence: 99%

“…5 Hence, the quantitative determination of rutin is of considerable interest and different analytical techniques, including capillary electrophoresis, 6 high-performance liquid chromatography (HPLC), 7 spectrophotometry, 8 sequential injection analysis 9 and chemiluminescence 10 have been applied to determine rutin in pharmaceutical drugs and biological samples. Some clinical investigations demonstrate that rutin has a broad range of medical functions, such as diluting the blood and reducing blood pressure and as a hemostatic, antitumor, and anti-bacterial drug and an anti-oxidant.…”

Section: Introductionmentioning

confidence: 99%

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A highly defective mesoporous carbon – ionic liquid paste electrode toward the sensitive electrochemical determination of rutin

2017

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In this paper, a highly defective mesoporous carbon (DMC) and room temperature ionic liquid 1-butyl-3methylimidazolium hexafluorophosphate (BMIM$PF 6 ) were applied to fabricate a novel carbon paste electrode for the electrochemical sensing of rutin. The electrochemical properties of rutin on the modified electrode (IL/DMC/PE) were carefully investigated by cyclic voltammetry, electrochemical impedance spectroscopy and chronocoulometry. Electrochemical parameters of rutin on the surface of the modified electrode were determined with the electron transfer coefficient (a) as 0.53, the electron transfer number (n) as 2, the heterogeneous electron transfer rate constant (k s ) as 37.1 s À1 , the diffusion coefficient as 1.34 Â 10 À4 cm 2 s À1 and saturated absorption capacity as 10.9 nmol cm À2 . Under the optimal conditions, rutin could be detected in the concentration range from 0.008 mmol L À1 to 4.0 mmol L À1 with the detection limit as 1.17 nmol L À1 (S/N ¼ 3) by square-wave voltammetry. The proposed sensor was further applied successfully for the determination of rutin content in some real samples, including Ruta graveolens extract, pharmaceutical tablets and orange juice.

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“…[2] Rutin is a flavonoid that consists of aglycone quercetin and the disaccharide rutinose (α-L-rhamnopyranosyl-(1→6)-β-Dglucopyranose) bound to the aglycone hydroxyl group at the C-3 position. [3] The increase of secondary metabolites in plants in response to biotic and abiotic stress stimuli can give rise to adaptation to a variety of environmental conditions. [4,5] They are mediators of the stress responses in plants.…”

Section: Introductionmentioning

confidence: 99%

Determination of phenolic acids and rutin inHeliotropium thermophilumby high-performance liquid chromatography with photodiode array detection

Yıldırım

Kadıoğlu

Sağlam

2016

Instrumentation Science & Technology

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A novel and rapid method was validated for the determination of phenolics in plants by high performance liquid chromatography coupled to photodiode array detection. The optimized method was employed for the quantification of phenolic acids and rutin present in Heliotropium thermophilum. Eight samples collected from two locations were analyzed. Additionally, temperature dependent changes in the phenolic composition of H. thermophilum samples was evaluated. A series of standards were used for identification that includedprotocatechuic acid, 4-hydroxybenzoic acid, chlorogenic acid, vanillic acid, caffeic acid, syringic acid, p-coumaric acid, rutin, ferulic acid, benzoic acid, 2-hydroxycinnamic acid, abscisic acid, and trans-cinnamic acid. Chromatographic separation was performed on C 8 column at 40 °C temperature with gradient elution. A phosphate buffer (20 mM, pH: 2.5) and acetonitrile mixture was used as the mobile phase with detection at 280 nm wavelength and a flow rate of 1.5 mL/min. The developed method was shown to be sensitive, accurate, and reproducible for the determination of phenolic acids and rutin in plants.

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Application of HPCCC, UHPLC-PDA-ESI-MS 3 and HPLC-PDA methods for rapid, one-step preparative separation and quantification of rutin in Forsythia flowers

Cited by 27 publications

References 34 publications

Green-Sustainable Recovery of Phenolic and Antioxidant Compounds from Industrial Chestnut Shells Using Ultrasound-Assisted Extraction: Optimization and Evaluation of Biological Activities In Vitro

Green-Sustainable Recovery of Phenolic and Antioxidant Compounds from Industrial Chestnut Shells Using Ultrasound-Assisted Extraction: Optimization and Evaluation of Biological Activities In Vitro

A highly defective mesoporous carbon – ionic liquid paste electrode toward the sensitive electrochemical determination of rutin

Determination of phenolic acids and rutin inHeliotropium thermophilumby high-performance liquid chromatography with photodiode array detection

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