Application of Hot Start PCR Method in PCR-based Preimplantation Genetic Diagnosis

Abstract

Genetic Medicine of single gene defects. 2,3) However, PCR using a single cell cause errors in diagnosis, such as allele dropout because of a low con centration of DNA. 4) The Taq polymerase included with PCR regents has especially high activity at 37℃. 5) In front case, the researchers may obtain a result in which they fail to amplify the correct band or get to obtain nonspecific bands because of a high mismatch with primers. Thus, fine-tuning PCR conditions can be an intensive process that is susceptible to all the problems of conventional PCR. 3) The hot start PCR method has been reported to decrease the amount of nonspecific products and primer artifacts. 6) In brief,