Application of highly ozonated sunflower oil does not improve palatal wound healing: A randomized controlled clinical trial

Abstract

Background Ozone is a molecule that plays an important role in dentistry, specially for wound healing. The aim of the present study was to clinically and immunologically evaluate the effect of ozonated oil on the healing of palatal wounds. Methods This is a prospective, longitudinal, triple‐blind, randomized, placebo‐controlled clinical trial. The groups were divided as follows: Test group (n = 14): after removal of the free gingival graft (FGG), the palatal wound was treated with ozonized seed sunflower oil with a peroxide index between 510 and 625 meq O2/kg; Control group (n = 14): after removal of the FGG, the palatal wound was treated with non‐ozonated sunflower oil (placebo). The treatments were applied three times a day, for 7 days. Results There were no significant differences in the measurements of wound area (mm2) between the test and control groups in the different periods evaluated (0, 3, 7, and 14 days; p > 0.05). The intra‐group analysis showed a significant decrease in wound size over the course of days (0, 3, 7, and 14 days; p < 0.05). Vascular endothelial growth factor (VEGF; pg/mL) presented a significant reduction at 7 days (p < 0.05) compared to day 3 in the test group (p < 0.05). There was a statistical difference for malondialdehyde (MDA; pg/mL) in the test group between 3 and 7 days post‐treatment (p < 0.05) and between test and control groups on the 7th day (p < 0.05). Conclusions The application of highly ozonated sunflower oil did not improve the remaining scar area of the palate, decreasing the VEGF and increasing the oxidative stress marker MDA.

Wound‐healing agents for palatal donor area: A network meta‐analysis

“…However, variable parameters in ozone concentration, forms application (water, oil, or gaseous), and treatment interval were used in the included studies making the comparison between the studies unreliable. In addition, the application of highly ozonated sunflower oil did not improve the remaining scar area (Loureiro et al., 2023) and postoperative pain of the palate (Juber et al., 2023), promoting worse quality of life scores in comparison to non‐ozonated sunflower oil (Juber et al., 2023). These clinical results were associated with a decrease in the VEGF levels and an increase in the oxidative stress marker malondialdehyde (MDA) (Loureiro et al., 2023).…”

“…In addition, the application of highly ozonated sunflower oil did not improve the remaining scar area (Loureiro et al., 2023) and postoperative pain of the palate (Juber et al., 2023), promoting worse quality of life scores in comparison to non‐ozonated sunflower oil (Juber et al., 2023). These clinical results were associated with a decrease in the VEGF levels and an increase in the oxidative stress marker malondialdehyde (MDA) (Loureiro et al., 2023). According to the authors, the peroxide content of the oil used (peroxide index: 510 and 625 meq/kg), the mode of application (by the patient with a cotton swab), and the frequency of use (3 times a day) have negatively influenced the results (Juber et al., 2023; Loureiro et al., 2023).…”

“…These clinical results were associated with a decrease in the VEGF levels and an increase in the oxidative stress marker malondialdehyde (MDA) (Loureiro et al, 2023). According to the authors, the peroxide content of the oil used (peroxide index: 510 and 625 meq/ kg), the mode of application (by the patient with a cotton swab), and the frequency of use (3 times a day) have negatively influenced the results (Juber et al, 2023;Loureiro et al, 2023).…”