Application of High-Resolution MALDI-TOFMS with a Spiral Ion Trajectory for the Structural Characterization of Free Radical Polymerized Methacrylate Ester Copolymers

Sato, Hiroaki; Ishii, Yoko; Momose, Hikaru; Sato, Takafumi; Teramoto, Kanae

doi:10.5702/massspectrometry.a0014

Cited by 14 publications

(4 citation statements)

References 22 publications

(26 reference statements)

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“…17 m ight path (JMS-S3000, JEOL, Tokyo, Japan). [15][16][17] e ionization was carried out with Nd:YLF laser (349 nm) in 0.5 ns pulse width and mainly 250 Hz frequency. Ions generated by the laser desorption were introduced into the ight tube with an accelerating voltage of 20 kV and separated in a positive spiral mode.…”

Section: Maldi-msmentioning

confidence: 99%

Complementary Characterization of End Groups in Radically Polymerized Poly(methyl methacrylate) by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry and Pyrolysis-Gas Chromatography-Mass Spectrometry

Ohtani

Iura

2014

Mass Spectrometry

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e end groups in radically polymerized poly(methyl methacrylate) samples with tert-butyl peroxy-2-ethylhexanoate as an aliphatic peroxide initiator and 1-octanethiol as a chain transfer reagent were complementarily characterized by high-resolution matrix assisted laser desorption/ionization (MALDI) spiral time-ofight mass spectrometry (MS) and pyrolysis-gas chromatography-mass spectrometry (Py-GC-MS). e end groups comprised of three types of the initiator fragments and octylthio group originating from the chain transfer agent were con rmed by MALDI-MS measurements. In addition, their quantitative information was obtained by Py-GC-MS. Furthermore, combined with size exclusion chromatographic fractionation, the molar mass dependence of the end groups in the PMMA samples was also examined. It was suggested that the relative content of the octylthio end groups might increase with increase in the molar mass of the fractions. e observed results were interpreted in terms of the polymerization reactions of the PMMA samples.

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Section: Maldi-msmentioning

confidence: 99%

Complementary Characterization of End Groups in Radically Polymerized Poly(methyl methacrylate) by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry and Pyrolysis-Gas Chromatography-Mass Spectrometry

Ohtani

Iura

2014

Mass Spectrometry

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“…Therefore, we utilized a JMS-S3000 Spiral-TOF with the same MALDI ionization method and a spiral ion trajectory of 17 m to improve the resolution and accuracy of TOF. 15 Second, in terms of the sodium adduct ions, contamination by extraneous sodium ions, such as from scientific glassware, different measurement samples (steroids) and the derivatization reagent (DNPH), could be a problem. Third, DNPH and 3-hydroxy-2-naphthoic acid hydrazide could be thought to possess different activities as reactive matrices in MALDI-MS.…”

Section: Resultsmentioning

confidence: 99%

Hydrazide and Hydrazine Reagents as Reactive Matrices for Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry to Detect Steroids with Carbonyl Groups

Shigeri

Yasuda

Sakai

et al. 2015

Eur J Mass Spectrom (Chichester)

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Fifty-one reagents (37 hydrazides and 14 hydrazines) were examined to determine if they could function as reactive matrices for the detection of steroids with carbonyl groups in matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). Among these reagents, 3-hydroxy-2-naphthoic acid hydrazide reacted most efficiently with steroids with carbonyl groups, allowing detection of them at the 1pmol level. Results from accurate mass measurement with a JMS-S3000 Spiral-TOF suggested that the ion peaks corresponding to the protonated fragment of a hydrazone were observed, in addition to the protonated molecular peaks of the resulting hydrazones ([M(D)+H](+)) and their sodium adduct ([M(D)+Na](+)). 3-Hydroxy-2-naphthoic acid hydrazide also could detect cortisol (1.93ng) spiked in a human serum certified reference material, as well as cortisone and cortisol in a human saliva sample collected in the morning. Using a combination of 3-hydroxy-2-naphthoic acid hydrazide and α- cyano-4-hydroxycinammic acid (CHCA) (3-hydroxy-2-naphthoic acid hydrazide:CHCA=4:1, v/v), even cortisone and cortisol in a human saliva sample collected in the afternoon, when levels are typically low, could be detected effectively.

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“…This can be overcome using a new type of high-resolution TOFMS instrument with a spiral ion trajectory (spiral-TOFMS) in which the long flight path of the ions along the spiral ion trajectory of approximately 17 m (2.1 m Â 8 turns) gives a high mass-resolving power of 80 000 at full width at half maximum for m/z 2564 of a peptide sample. 19 MALDI spiral-TOFMS, which has easily succeeded in separating adjacent peaks (D 0.036 Da) of copolymers up to m/z 3000 20 will be useful for structure assignment of individual mycolic acids.…”

Section: Introductionmentioning

confidence: 99%

Simple and rapid characterization of mycolic acids from Dietzia strains by using MALDI spiral-TOFMS with ultra high mass-resolving power

et al. 2013

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Mycolic acids have been used as important chemotaxonomic markers. In this study, a newly developed matrix-assisted laser desorption/ionization time-of-flight mass spectrometer with a spiral ion trajectory (MALDI spiral-TOFMS) was applied to the characterization of mycolic acids of three type strains of validated species belonging to the genus Dietzia (D. papillomatosis 105045(T), D. kunjamensis NBRC 105042(T) and D. timorensis NBRC 104184(T)), by analysis of total fatty acid methyl ester fractions. In addition, owing to the high mass-resolving power of MALDI spiral-TOFMS, adjacent peaks (0.036 Da mass differences) were successfully separated, and weak peaks corresponding to oxygenated mycolic acids were detected. For all samples, the distributions of carbon-chain lengths were mainly in the range of C30-C42 and the average number of carbon-chain lengths was about 37, which agreed reasonably well with reported results for the genus Dietzia. The number of double bonds and/or cyclopropane rings was 0-2. Relative peak intensities of each mycolic acid methyl ester were used to compare the mycolic acids of the three strains. The mycolic acids of D. papillomatosis and D. kunjamensis were characterized by a high content of mycolic acids with 0-1 double bond or cyclopropane ring and an almost equal content of mycolic acids with odd- and even-numbered carbon-chain lengths. In contrast, mycolic acids of D. timorensis were characterized by a high content of mycolic acids with 1-2 double bonds and/or cyclopropane rings with an even-numbered carbon-chain length. By using MALDI spiral-TOFMS, mycolic acids from three type strains of the genus Dietzia were characterized easily and rapidly.

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Application of High-Resolution MALDI-TOFMS with a Spiral Ion Trajectory for the Structural Characterization of Free Radical Polymerized Methacrylate Ester Copolymers

Cited by 14 publications

References 22 publications

Complementary Characterization of End Groups in Radically Polymerized Poly(methyl methacrylate) by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry and Pyrolysis-Gas Chromatography-Mass Spectrometry

Complementary Characterization of End Groups in Radically Polymerized Poly(methyl methacrylate) by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry and Pyrolysis-Gas Chromatography-Mass Spectrometry

Hydrazide and Hydrazine Reagents as Reactive Matrices for Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry to Detect Steroids with Carbonyl Groups

Simple and rapid characterization of mycolic acids from Dietzia strains by using MALDI spiral-TOFMS with ultra high mass-resolving power

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