Application of genomics and proteomics for identification of bacterial gene products as potential vaccine candidates

Genome-Based Bioinformatic Selection of ChromosomalBacillus anthracisPutative Vaccine Candidates Coupled with Proteomic Identification of Surface-Associated Antigens

“…Reducing the number of candidates to be evaluated experimentally, to a manageable number, would involve application of a high throughput biological screening system (such as proteomicbased analysis of in vivo-expressed immunogens or in vitro-in vivo expression systems [7,14,25]); and/or application of additional computational steps directed toward selection of microorganism-specific genes.…”

Postgenomic Analysis of Four Novel Antigens of Group A Streptococcus : Growth Phase-Dependent Gene Transcription and Human Serologic Response

“…For more than 40 years, it has been known that patients with GAS infections produce antibodies to a large number of extracellular proteins (16)(17)(18)(19)(20)24), but most of these antigens have not been identified. Availability of genome sequences has permitted virulence factors and therapeutic agent candidates to be identified very rapidly by comparative genomics and other postgenomic methods (2,3,14,21,24,32,35,37). The studies reported here were stimulated by a recent analysis of four genomes of GAS which led to the discovery of four previously undescribed genes encoding extracellular proteins (37).…”

Immunome-derived vaccines

“…Most of these protein structure-querying tools are available on the ExPASy website [101]. Several reviews of bioinformatics tools for vaccine design have been published [12,13].…”