Application of genetic screening processor in screening neonatal glucose-6-phosphate dehydrogenase deficiency

Miao, H.; Peng, Yichuan; Shi, Yezhen; Zhang, Ting; Chen, Rongqing; Wu, Dingwen; Yang, Rong; Huang, Xinwen

doi:10.3724/zdxbyxb-2021-0275

Cited by 1 publication

(1 citation statement)

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“…Mouse hemocytes were obtained from the eyeball vein. The portions of the blood cells were detected in terms of the number of leukocytes, lymphocytes, monocytes, and neutrophils by a semi-automatic blood biochemical analyzer (Mindray, Shenzhen, China) as described previously [ 23 ]. Serum precipitation from another portion of blood was used to detect the content of IL-6, TNF-α, IL-1β, and IL-17 in mouse serum by commercial ELISA kits (R&D Systems, USA) according to the manufacturer’s instructions.…”

Section: Methodsmentioning

confidence: 99%

Anti-Inflammatory and Immunomodulatory Effects of 0.1 Sub-Terahertz Irradiation in Collagen-Induced Arthritis Mice

Zhang,

Shang,

et al. 2024

IJMS

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The primary emphasis of photoimmunology is the impact of nonionizing radiation on the immune system. With the development of terahertz (THz) and sub-terahertz (sub-THz) technology, the biological effects of this emerging nonionizing radiation, particularly its influence on immune function, remain insufficiently explored but are progressively attracting attention. Here, we demonstrated that 0.1 sub-THz radiation can modulate the immune system and alleviate symptoms of arthritis in collagen-induced arthritis (CIA) mice through a nonthermal manner. The application of 0.1 sub-THz irradiation led to a decrease in proinflammatory factors within the joints and serum, reducing the levels of blood immune cells and the quantity of splenic CD4+ T cells. Notably, 0.1 sub-THz irradiation restored depleted Treg cells in CIA mice and re-established the Th17/Treg equilibrium. These findings suggested that sub-THz irradiation plays a crucial role in systemic immunoregulation. Further exploration of its immune modulation mechanisms revealed the anti-inflammatory properties of 0.1 sub-THz on LPS-stimulated skin keratinocytes. Through the reduction in NF-κB signaling and NLRP3 inflammasome activation, 0.1 sub-THz irradiation effectively decreased the production of inflammatory factors and immune-active substances, including IL-1β and PGE2, in HaCaT cells. Consequently, 0.1 sub-THz irradiation mitigated the inflammatory response and contributed to the maintenance of immune tolerance in CIA mice. This research provided significant new evidence supporting the systemic impacts of 0.1 sub-THz radiation, particularly on the immune system. It also enhanced the field of photoimmunology and offered valuable insights into the potential biomedical applications of 0.1 sub-THz radiation for treating autoimmune diseases.

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Section: Methodsmentioning

confidence: 99%