Application of gelatin-coated magnetic particles for isolation of genomic DNA from bacterial cells

Modification of gelatin–DNA interaction for optimised DNA extraction from gelatin and gelatin capsule

“…Moreover, the DNA extraction process is restricted by the strong interaction of DNA–gelatin complexes . Unsurprisingly, gelatin has also been a well‐known carrier for DNA and has been used to assist in DNA precipitation . Thus, the interaction of gelatin and DNA should be interrupted in order to make the free DNA available for DNA precipitation.…”

Bench-To-Bedside Translation of Magnetic Nanoparticles

“…Magnetic separation is widely used for the separation and purification of cells [89], recombinant proteins [90], DNA [91] and monoclonal antibodies [92]. Separation of these biological constituents using MNPs involves attachment of biological constituents to surface-modified MNPs, followed by separation with the aid of an external magnetic force [93].…”

N-Methylimidazolium modified magnetic particles as adsorbents for solid phase extraction of genomic deoxyribonucleic acid from genetically modified soybeans

“…In MSPE, the adsorbents with a magnetic core need not be packed into the SPE cartridges, and centrifuge steps can be replaced by an external magnetic field to achieve solid-liquid separation. So far, many kinds of surface modification materials, such as chitosan (CS) [7], polyethylenimine (PEI) [8], tetraethoxysilane (TEOS) [9,10], poly(glycidyl methacrylate) (PGMA) [11], and gelatin [12], were used for preparation of functionalized magnetic particles (MPs) as adsorbents for extraction of nucleic acid. Nonetheless, the application of MSPE in extraction of nucleic acid also faces the problem of mass loss of nucleic acid during the extraction process, making the productivity of nucleic acid much lower than traditional liquid phase methods.…”