Application of FTIR spectroscopy in ecotoxicological studies supported by multivariate analysis and 2D correlation spectroscopy

Mecozzi, Mauro; Pietroletti, Marco; Mento, Rossella Di

doi:10.1016/j.vibspec.2006.11.006

Cited by 39 publications

(18 citation statements)

References 14 publications

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“…5). The biological characteristic bands, including the acyl vibration bands from fatty acids and lipids, amide I and II protein vibration bands, nucleic acid vibration bands, and phospholipids (22), were recognizable and are highlighted in Fig. 5.…”

Section: Vol 192 2010 Impact Of Silver(i) On Metabolism Of S Oneidmentioning

confidence: 99%

Impact of Silver(I) on the Metabolism of Shewanella oneidensis

et al. 2010

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Anaerobic cultures of Shewanella oneidensis MR-1 reduced toxic Ag(I), forming nanoparticles of elemental Ag(0), as confirmed by X-ray diffraction analyses. The addition of 1 to 50 M Ag(I) had a limited impact on growth, while 100 M Ag(I) reduced both the doubling time and cell yields. At this higher Ag(I) concentration transmission electron microscopy showed the accumulation of elemental silver particles within the cell, while at lower concentrations the metal was exclusively reduced and precipitated outside the cell wall. Whole organism metabolite fingerprinting, using the method of Fourier transform infrared spectroscopy analysis of cells grown in a range of silver concentrations, confirmed that there were significant physiological changes at 100 M silver. Principal component-discriminant function analysis scores and loading plots highlighted changes in certain functional groups, notably, lipids, amides I and II, and nucleic acids, as being discriminatory. Molecular analyses confirmed a dramatic drop in cellular yields of both the phospholipid fatty acids and their precursor molecules at high concentrations of silver, suggesting that the structural integrity of the cellular membrane was compromised at high silver concentrations, which was a result of intracellular accumulation of the toxic metal.

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Section: Vol 192 2010 Impact Of Silver(i) On Metabolism Of S Oneidmentioning

confidence: 99%

Impact of Silver(I) on the Metabolism of Shewanella oneidensis

et al. 2010

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“…Infra-Red spectroscopy has already been used on algae to analyze nutrients, to identify polysaccharides structures or fucoidans [27] and also to get a physiological fingerprint of the potential growth of microalgae. Ecotoxicological studies can also benefit from this technique, which allows the observation of metal [28] . Considerable technological advances on this process now allow the use of FTIR as a quantification tool for evaluating macromolecules composition changes in microalgae [29] .…”

Section: Introductionmentioning

confidence: 99%

Seasonal Variation of Sargassum Muticum Biochemical Composition Determined by Fourier Transform Infra-Red Spectroscopy

Vandanjon¹,

Déniel²,

Puspita³

et al. 2017

JABST

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Proliferation of the introduced brown macro alga Sargassum muticum is known as a natural and hard to control phenomenon occurring along the Atlantic coasts. The phenomenon causes serious troubles for local ecosystems including the alteration of ecosystem structure, the reduction in indigenous biodiversity and economic losses (tourism, aquaculture). However, despite the serious troubles caused by S. muticum, this species contains highly remarkable bioactive metabolites. This macro alga is at present under-exploited and the valorization of its metabolites to give a positive value to this seaweed could be a solution of ecosystemic service. Biorefinery process could be one solution to valorize S. muticum. Comprehensive knowledge concerning the biochemical composition of S. muticum and the impact of environmental factors, particularly seasons, on its composition is a prerequisite before its valorization. In this study, the biochemical composition of S. muticum was evaluated by using classic colorimetric methods based on chemical analysis and Fourier Transform Infra-Red spectrometry (FTIR), and was used as a rapid and safe method that could bring advantages in screening studies and a more comprehensive management and use of seaweed products. Our results are globally in accordance, notably for phenolic compounds, showing the relevance of the use of infrared spectrometry. Moreover, based on the absorption bands of some specific and valuable compounds shown by FTIR, there was a seasonal variation in the polysaccharides, i.e., uronic acids and sulphated compounds, together with phenolic contents of S. muticum.

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“…The application of chemometric methods like PCA and LDA allows the extraction of key features from the IR spectrum in the form of loadings and cluster vectors, which denote which regions of the IR spectrum are responsible for segregation between control and treated cells when viewed alongside the scores plots (Baker et al, 2014;Martin et al, 2010;Trevisan et al, 2012). The combination of IR spectroscopy and multivariate techniques for feature extraction has previously been applied in human, algal and bacterial cell types in order to distinguish between treated and control cells and generate potential biomarkers based upon the loadings and cluster vectors generated Johnson et al, 2014;Llabjani et al, 2010Llabjani et al, , 2011Mecozzi et al, 2007;Riding et al, 2012a;Ukpebor et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Infrared spectroscopy detects changes in an amphibian cell line induced by fungicides: Comparison of single and mixture effects

et al. 2016

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Contact CEH NORA team at noraceh@ceh.ac.ukThe NERC and CEH trademarks and logos ('the Trademarks') are registered trademarks of NERC in the UK and other countries, and may not be used without the prior written consent of the Trademark owner. Distinct differences were apparent in the IR spectra of cells exposed to carbendazim and those exposed to flusilazole, suggesting different mechanisms of action. Exposure to binary mixtures of carbendazim and flusilazole also induced significant spectral alterations, again in regions associated with phospholipids and proteins, but also in regions associated with DNA and carbohydrates. Overall these findings demonstrate that IR spectroscopy is a sensitive technique for examining the effects of environmentally-relevant levels of fungicides at the cellular level. The combination of IR spectroscopy with the A6 cell line could serve as a useful model to identify agents that might threaten amphibian health in a rapid and high throughput manner.

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Application of FTIR spectroscopy in ecotoxicological studies supported by multivariate analysis and 2D correlation spectroscopy

Cited by 39 publications

References 14 publications

Impact of Silver(I) on the Metabolism of Shewanella oneidensis

Impact of Silver(I) on the Metabolism of Shewanella oneidensis

Seasonal Variation of Sargassum Muticum Biochemical Composition Determined by Fourier Transform Infra-Red Spectroscopy

Infrared spectroscopy detects changes in an amphibian cell line induced by fungicides: Comparison of single and mixture effects

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