Application of fluorescent <i>in situ</i> hybridisation for demonstration of <i>Coxiella burnetii</i> in placentas from  ruminant abortions

Jensen, Tim Kåre; Montgomery, Donald L.; Jaeger, Paula T.; Lindhardt, Tina; Agerholm, Jørgen Steen; Bille‐Hansen, Vivi; Boye, Mette

doi:10.1111/j.1600-0463.2007.apm_591.x

Cited by 44 publications

(42 citation statements)

References 23 publications

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“…FISH has been used in veterinary reports to detect C. burnetii in ruminant placentas using both IS1111 and 16S rRNA probes (40,41). Its use to detect C. burnetii in human lymph nodes has only recently been published (34).…”

Section: Discussionmentioning

confidence: 99%

Mouse Model of Coxiella burnetii Aerosolization

et al. 2016

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cCoxiella burnetii is mainly transmitted by aerosols and is responsible for multiple-organ lesions. Animal models have shown C. burnetii pathogenicity, but long-term outcomes still need to be clarified. We used a whole-body aerosol inhalation exposure system to mimic the natural route of infection in immunocompetent (BALB/c) and severe combined immunodeficient (SCID) mice. After an initial lung inoculum of 10 4 C. burnetii cells/lung, the outcome, serological response, hematological disorders, and deep organ lesions were described up to 3 months postinfection. C. burnetii-specific PCR, anti-C. burnetii immunohistochemistry, and fluorescent in situ hybridization (FISH) targeting C. burnetii-specific 16S rRNA completed the detection of the bacterium in the tissues. In BALB/c mice, a thrombocytopenia and lymphopenia were first observed, prior to evidence of C. burnetii replication. In all SCID mouse organs, DNA copies increased to higher levels over time than in BALB/c ones. Clinical signs of discomfort appeared in SCID mice, so follow-up had to be shortened to 2 months in this group. At this stage, all animals presented bone, cervical, and heart lesions. The presence of C. burnetii could be attested in situ for all organs sampled using immunohistochemistry and FISH. This mouse model described C. burnetii Nine Mile strain spread using aerosolization in a way that corroborates the pathogenicity of Q fever described in humans and completes previously published data in mouse models. C. burnetii infection occurring after aerosolization in mice thus seems to be a useful tool to compare the pathogenicity of different strains of C. burnetii.

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Section: Discussionmentioning

confidence: 99%

Mouse Model of Coxiella burnetii Aerosolization

et al. 2016

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“…The design of specific probes has allowed the direct visualization of diverse bacterial species such as C. burnetii (20), Staphylococcus spp. (21), and Salmonella spp.…”

Section: Detection Of Growthmentioning

confidence: 99%

The Rebirth of Culture in Microbiology through the Example of Culturomics To Study Human Gut Microbiota

et al. 2015

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SUMMARY Bacterial culture was the first method used to describe the human microbiota, but this method is considered outdated by many researchers. Metagenomics studies have since been applied to clinical microbiology; however, a “dark matter” of prokaryotes, which corresponds to a hole in our knowledge and includes minority bacterial populations, is not elucidated by these studies. By replicating the natural environment, environmental microbiologists were the first to reduce the “great plate count anomaly,” which corresponds to the difference between microscopic and culture counts. The revolution in bacterial identification also allowed rapid progress. 16S rRNA bacterial identification allowed the accurate identification of new species. Mass spectrometry allowed the high-throughput identification of rare species and the detection of new species. By using these methods and by increasing the number of culture conditions, culturomics allowed the extension of the known human gut repertoire to levels equivalent to those of pyrosequencing. Finally, taxonogenomics strategies became an emerging method for describing new species, associating the genome sequence of the bacteria systematically. We provide a comprehensive review on these topics, demonstrating that both empirical and hypothesis-driven approaches will enable a rapid increase in the identification of the human prokaryote repertoire.

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“…A limited number of studies have been performed examining the presence of C. burnetii in bovine, ovine, and caprine aborts in different countries (Sanchez et al 2006;Szeredi et al 2006;Jensen et al 2007;Hazlett et al 2013). Sheep aborts were positive for the presence of C. burnetii in 3.7% of cases in Germany (Plagemann 1989), 1% in Switzerland (Chanton-Greutmann et al 2002), and 2% in Hungary (Szeredi et al 2006).…”

Section: Discussionmentioning

confidence: 99%

“…For infection with this agent, IHC has been applied more on the mothers and placentas than on foetal organs (Bildfell et al 2000;Sanchez et al 2006;Jensen et al 2007;Muskens et al 2012). Due to the fact that PCR revealed C. burnetii positivity in nine cases versus only four when using IHC in a study population of 100 bovine placentas with foetal abort viscera, Muskens et al (2012) advanced the idea that IHC may be less sensitive than PCR.…”

Section: Discussionmentioning

confidence: 99%

“…C. burnetii is generally stained using the Gimenez method in both clinical specimens and laboratory cultures (Gimenez 1964). Methods such as egg embryo examination, cell culture, laboratory animal experimentation, PCR, IHC, and in situ hybridisation are typically used to isolate or identify the agent (Maurin and Raoult 1999;Bildfell et al 2000;Jensen et al 2007).…”

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confidence: 99%

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Determination of Coxiella burnetii in bovine foetuses using PCR and immunohistochemistry

Özkaraca¹,

Çeribaşı²,

Çeribaşı³

et al. 2016

Vet. Med. - Czech

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This study was aimed at detection of Coxiella burnetii in bovine foetuses using polymerase chain reaction (PCR) and immunohistochemistry (IHC) and at an estimation of its frequency in Eastern Turkey. Stamp, Giemsa, and Gimenez stains were used in addition to PCR and IHC to determine the presence of C. burnetii in samples from 70 bovine foetuses. While the staining methods did not detect the agent by direct visualisation of C. burnetii on smears, PCR and IHC identified its presence in two of the foetuses. The distribution of antigens in these two foetuses was, in decreasing order of concentration, in the spleen, the thymus, the lungs, the liver, and the kidneys. We conclude that C. burnetii diagnosis in bovine foetuses can be reliably performed using PCR and IHC. In addition, the frequency of 1.42% of C. burnetii positivity in bovine foetuses reported here was the first time that the presence of this agent was determined in Eastern Turkey.

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Application of fluorescent in situ hybridisation for demonstration of Coxiella burnetii in placentas from ruminant abortions

Cited by 44 publications

References 23 publications

Mouse Model of Coxiella burnetii Aerosolization

Mouse Model of Coxiella burnetii Aerosolization

The Rebirth of Culture in Microbiology through the Example of Culturomics To Study Human Gut Microbiota

Determination of Coxiella burnetii in bovine foetuses using PCR and immunohistochemistry

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