Application of flat panel OLED display technology for the point-of-care detection of circulating cancer biomarkers

Katchman, Benjamin A.; Smith, John E.; Kesiraju, Sailaja; Lee, Yong-Kyun; O’Brien, Barry; Kaftanoglu, Korhan; Christen, Jennifer Blain; Anderson, Karen S.

doi:10.1038/srep29057

Cited by 33 publications

(21 citation statements)

References 53 publications

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“…Previously, we reported the performance of a fluorescence detection system that uses relatively expensive 25 mm fi in a single site configuration (Katchman et al, 2016; Obahiagbon et al, 2016; Smith et al, 2016). Here, we discuss the use of 3×3 mm filter The 3×3 mm fi were mounted directly on the LEDs and photodiodes using optically clear UV curing adhesive, (NOA63, Norland Prodcuts Inc., NJ, USA) as shown in Fig.…”

Section: Methodsmentioning

confidence: 99%

“…Previously, we have reported the use of organic light emitting diodes (OLEDs), adapted from flat panel display technology, as the excitation source in a single-plex detection system (Katchman et al, 2016; Obahiagbon et al, 2016; Smith et al, 2016). We demonstrated the detection of antibodies to human papillomavirus (HPV16) E7 protein using expensive interference filters Compared to single-plex systems, low-cost, multiplexed diagnostic platforms are useful for disease diagnosis, making it possible for simultaneous detection of multiple analytes from a single patient sample volume.…”

Section: Introductionmentioning

confidence: 99%

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A compact, low-cost, quantitative and multiplexed fluorescence detection platform for point-of-care applications

Smith

Zhu

Katchman

et al. 2018

Biosensors and Bioelectronics

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An effective method of combating infectious diseases is the deployment of hand-held devices at the point-of-care (POC) for screening or self-monitoring applications. There is a need for very sensitive, low-cost and quantitative diagnostic devices. In this study, we present a low-cost, multiplexed fluorescence detection platform that has a high sensitivity and wide dynamic range. Our system features inexpensive 3×3 mm interference filter with a high stopband rejection, sharp transition edges, and greater than 90% transmission in the passband. In addition to the filters we improve signal-to-noise ratio by leveraging time for accuracy using a charge-integration-based readout. The fluorescence sensing platform provides a sensitivity to photon flux of ∼1×104 photons/mm2sec and has the potential for 2 to 3 orders of magnitude improvement in sensitivity over standard colorimetric detection that uses colored latex microspheres. We also detail the design, development, and characterization of our low-cost fluorescence detection platform and demonstrate 100% and 97.96% reduction in crosstalk probability and filter cost, respectively. This is achieved by reducing fi r dimensions and ensuring appropriate channel isolation in a 2×2 array configuration. Practical considerations with low-cost interference fi system design, analysis, and system performance are also discussed. The performance of our platform is compared to that of a standard laboratory array scanner. We also demonstrate the detection of antibodies to human papillomavirus (HPV16) E7 protein, as a potential biomarker for early cervical cancer detection in human plasma.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A compact, low-cost, quantitative and multiplexed fluorescence detection platform for point-of-care applications

Smith

Zhu

Katchman

et al. 2018

Biosensors and Bioelectronics

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“…The array-based OLED was formed from multiple light-emitting elements (pixels) which were individually activated, to emit light at specific wavelengths and to enable multiple target detection. The same group proposed in Katchman et al (2016) a high-density fluorescence, programmable, multiplexed recognition compact miniaturized device for point-of-care molecular diagnostics. The OLED technology was combined with protein microarray technology and 10 pg/mL limit of detection was achieved for human IgG.…”

Section: Organic Electronic-based Designsmentioning

confidence: 99%

Miniaturization of fluorescence sensing in optofluidic devices

Măriuța

Colin

Barrot-Lattes

et al. 2020

Microfluid Nanofluid

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Successful development of a micro-total-analysis system (µTAS, lab-on-a-chip) is strictly related to the degree of miniaturization, integration, autonomy, sensitivity, selectivity, and repeatability of its detector. Fluorescence sensing is an optical detection method used for a large variety of biological and chemical assays, and its full integration within lab-on-a-chip devices remains a challenge. Important achievements were reported during the last few years, including improvements of previously reported methodologies, as well as new integration strategies. However, a universal paradigm remains elusive. This review considers achievements in the field of fluorescence sensing miniaturization, starting from off-chip approaches, representing miniaturized versions of their lab counter-parts, continuing gradually with strategies that aim to fully integrate fluorescence detection on-chip, and reporting the results around integration strategies based on optical-fiber-based designs, optical layer integrated designs, CMOS-based fluorescence sensing, and organic electronics. Further successful development in this field would enable the implementation of sensing networks in specific environments that, when coupled to Internet-of-Things (IoT) and artificial intelligence (AI), could provide real-time data collection and, therefore, revolutionize fields like health, environmental, and industrial sensing.

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“…Especially, significant efforts have been oriented toward downscaling the complex integrated structures, diversifying the target molecules, and enhancing the detection performances. Recently, a research group at Arizona State University combined OLED display technology with protein microarray technology to demonstrate high‐density multiplexed fluorescent biorecognition . Using this method, a clinical‐level sensitivity comparable with that of established enzyme linked immunosorbent assay (ELISA) was achieved in a thin transparent point‐of‐care platform, most notably for the detection of circulating cancer biomarkers (serum antibodies against HPV16 E2, E6, and E7).…”

Section: Progress Summarymentioning

confidence: 99%

“…Recently, a research group at Arizona State University combined OLED display technology with protein microarray technology to demonstrate high-density multiplexed fluorescent biorecognition. 37,38 Using this method, a clinical-level sensitivity comparable with that of established enzyme linked immunosorbent assay (ELISA) was achieved in a thin transparent point-of-care platform, most notably for the detection of circulating cancer biomarkers (serum antibodies against HPV16 E2, E6, and E7). Another interesting demonstration is found in the paper by Venkatraman and Steckl published in 2017 36 (Figure 3).…”

Section: Display-based Sensing Unitmentioning

confidence: 99%

Display meets biology: A vision for ubiquitous healthcare platforms

2019

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Ever‐increasing demand for a healthy and sustainable life has been a traditional motivation behind state‐of‐the‐art medicines and precision diagnosis tools. Nowadays, the ultra‐connectivity and data‐centric initiatives that underpin the future Internet of Things is seen as another major driver of electronically enriched, highly customizable healthcare platforms. Especially, the information display technology, which already enjoys its position as an all‐round player in consumer electronics is poised to broaden its territory by envisioning a range of bio‐related features. In this review, the rising opportunities for next‐generation display‐based biotechnology and healthcare systems are critically assessed in light of recent literature. Clearly, smart combination of new materials, devices, and integration strategies has given rise to highly promising prototypes, and this article aims at highlighting some of these examples. Despite such progress, it is still unclear whether the resultant technologies will make a measurable impact in the near future, given both their own scientific limits and the lack of proper business model. In this regard, the final part of this contribution is dedicated to emergent challenges as well as development solutions that this potentially disruptive innovation may need to consider.

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Application of flat panel OLED display technology for the point-of-care detection of circulating cancer biomarkers

Cited by 33 publications

References 53 publications

A compact, low-cost, quantitative and multiplexed fluorescence detection platform for point-of-care applications

A compact, low-cost, quantitative and multiplexed fluorescence detection platform for point-of-care applications

Miniaturization of fluorescence sensing in optofluidic devices

Display meets biology: A vision for ubiquitous healthcare platforms

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