Application of First Derivative Fluorescence Spectrometry to the Simultaneous Determination of Paracetamol and Salicylamide in Pharmaceuticals

Murillo, J. A.; García, Luciano

doi:10.1080/00032719608000408

Cited by 21 publications

(13 citation statements)

References 12 publications

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“…Therefore, it was desirable to develop simple and fast procedures that could be applied in quality control laboratories for the determination of MLT in presence of compound 10 . Derivative spectrofluorimetry and multivariate calibration methods such as PCR and PLS are useful means of resolving two overlapping spectra and eliminating matrix interference in the assay of two-component mixtures [13, 14]. …”

Section: Introductionmentioning

confidence: 99%

New spectrofluorimetric methods for determination of melatonin in the presence of N-{2-[1-({3-[2-(acetylamino)ethyl]-5-methoxy-1H-indol-2-yl}methyl)-5-methoxy-1H-indol-3-yl]- ethyl}acetamide: a contaminant in commercial melatonin preparations

Darwish

Attia

Zlotos

2012

Chemistry Central Journal

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BackgroundMelatonin (MLT) has many health implications, therefore it is of valuable importance to develop specific analytical methods for determination of MLT in the presence of its main contaminant, N-{2-[1-({3-[2-(acetylamino)ethyl]-5-methoxy-1H-indol-2-yl}methyl)-5-methoxy-1H-indol-3-yl]ethyl}acetamide (10). For development of these analytical methods, compound 10 had to be prepared in an adequate amount.ResultsCompound 10 was synthesized in six steps starting from 5-methoxyindole-2-carboxylic acid (1). Analytical performance of the proposed spectrofluorimetric methods was statistically validated with respect to linearity, accuracy, precision and specificity. The proposed methods were successfully applied for the assay of MLT in laboratory prepared mixtures containing up to 60 % of compound 10 and in commercial MLT tablets with recoveries not less than 99.00 %. No interference was observed from common pharmaceutical additives and the results were favorably compared with those obtained by a reference method.ConclusionsThis work describes simple, sensitive, and reliable second derivative spectrofluorimetric method in addition to two multivariate calibration methods, principal component regression (PCR) and partial least square (PLS), for the determination of MLT in the presence of compound 10.

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Section: Introductionmentioning

confidence: 99%

New spectrofluorimetric methods for determination of melatonin in the presence of N-{2-[1-({3-[2-(acetylamino)ethyl]-5-methoxy-1H-indol-2-yl}methyl)-5-methoxy-1H-indol-3-yl]- ethyl}acetamide: a contaminant in commercial melatonin preparations

Darwish

Attia

Zlotos

2012

Chemistry Central Journal

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“…The probable reaction as shown in fig. 1, N-acetyl-p-quinone imine was only produced by the oxidation of paracetamol, 2,2-dihydroxy-5,5’-diacetylaminobiphenyl is formed only in alkaline medium2526.…”

Section: Resultsmentioning

confidence: 99%

Novel atomic absorption spectrometric and rapid spectrophotometric methods for the quantitation of paracetamol in saliva: Application to pharmacokinetic studies

Issa¹,

Nejem²,

El-Abadla³

et al. 2008

Indian J Pharm Sci

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A novel atomic absorption spectrometric method and two highly sensitive spectrophotometric methods were developed for the determination of paracetamol. These techniques based on the oxidation of paracetamol by iron (III) (method I); oxidation of p-aminophenol after the hydrolysis of paracetamol (method II). Iron (II) then reacts with potassium ferricyanide to form Prussian blue color with a maximum absorbance at 700 nm. The atomic absorption method was accomplished by extracting the excess iron (III) in method II and aspirates the aqueous layer into air-acetylene flame to measure the absorbance of iron (II) at 302.1 nm. The reactions have been spectrometrically evaluated to attain optimum experimental conditions. Linear responses were exhibited over the ranges 1.0-10, 0.2-2.0 and 0.1-1.0 μg/ml for method I, method II and atomic absorption spectrometric method, respectively. A high sensitivity is recorded for the proposed methods I and II and atomic absorption spectrometric method value indicate: 0.05, 0.022 and 0.012 μg/ml, respectively. The limit of quantitation of paracetamol by method II and atomic absorption spectrometric method were 0.20 and 0.10 μg/ml. Method II and the atomic absorption spectrometric method were applied to demonstrate a pharmacokinetic study by means of salivary samples in normal volunteers who received 1.0 g paracetamol. Intra and inter-day precision did not exceed 6.9%.

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“…Spectrofluorimetric method proved to be more selective than normal UV-spectroscopy due to quantitation of substance at characteristic excitation and emission wavelengths. Derivative spectrofluorimetry provides a greater selectivity and spectral discrimination than common spectrofluorimetry [14,15]. It is a powerful approach for resolution of one analyte whose peak is hidden by a large overlapping peak of another analyte in multi-component analysis.…”

Section: Introductionmentioning

confidence: 99%

Development and validation of spectrofluorimetric method for determination of diflunisal and its impurity

et al. 2016

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A new sensitive, simple, rapid, accurate and precise spectrofluorimetric method for determination of diflunisal and its impurity is developed. Determination of diflunisal is based on first derivative spectrofluorimetric method, while its impurity can be determined by zero order spectrofluorimetric method. Diflunisal was measured at zero-crossing wavelength 394nm (zero crossing point with its impurity) which was selected for quantification of diflunisal. The impurity was measured directly at 334 nm, using 0.05 M phosphate buffer (pH = 9) as solvent. The analytical signal resulting from first derivative and zero order spectra were measured for diflunisal and its impurity, respectively. Linearity was over the range of 0.1-0.9 μg/mL for both with detection limit of 0.02 and 0.03 μg/mL and quantitation limit of 0.07 and 0.09 μg/mL for diflunisal and its impurity, respectively. The proposed method was validated as per ICH guidelines.The accuracy was checked by applying the proposed method for the determination of the drug and its impurity, the mean percentage recoveries were found to be 99.61±0.911 and 100.41±1.373 for diflunisal and its impurity, respectively. RSD values for repeatability testing were 0.268 and 0.569 and for intermediate precision were 0.224 and 0.259 for diflunisal and its impurity, respectively. The proposed method was effectively applied to analysis of studied drug in its tablet formulation. The results obtained by it were statistically compared with the reported method revealing high accuracy and good precision.

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Application of First Derivative Fluorescence Spectrometry to the Simultaneous Determination of Paracetamol and Salicylamide in Pharmaceuticals

Cited by 21 publications

References 12 publications

New spectrofluorimetric methods for determination of melatonin in the presence of N-{2-[1-({3-[2-(acetylamino)ethyl]-5-methoxy-1H-indol-2-yl}methyl)-5-methoxy-1H-indol-3-yl]- ethyl}acetamide: a contaminant in commercial melatonin preparations

New spectrofluorimetric methods for determination of melatonin in the presence of N-{2-[1-({3-[2-(acetylamino)ethyl]-5-methoxy-1H-indol-2-yl}methyl)-5-methoxy-1H-indol-3-yl]- ethyl}acetamide: a contaminant in commercial melatonin preparations

Novel atomic absorption spectrometric and rapid spectrophotometric methods for the quantitation of paracetamol in saliva: Application to pharmacokinetic studies

Development and validation of spectrofluorimetric method for determination of diflunisal and its impurity

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