Application of fed‐batch culture to citric acid production by <i>Aspergillus niger</i>: The effects of dilution rate and dissolved oxygen tension

Dawson, Mark W.; Maddox, I. S.; Boag, I. F.; Brooks, J.

doi:10.1002/bit.260320212

Cited by 42 publications

(10 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The respective concentration levels of nitrogen and phosphorous reported in the studies (Dawson et al 1988, Dawson et al 1989, Kristiansen and Sinclair, 1979) employing short fermentation periods are lower than those reported in the studies (Xu et al 1989a, Hossain et al 1984, Kristiansen and Sinclair, 1978 employing long fermentation periods. These observations suggest that optimum levels of nitrogen and phosphorous sources for citric acid fermentation vary with fermentation periods.…”

Section: Effect Of Cultivation Termscontrasting

confidence: 79%

“…This result is in agreement with that of Kristiansen and Charley (1981), who considered the level of excess nitrogen, rather than the lack of phosphorous was the more important factor. These similar findings may explain why the phenomenon of nitrogen catabolite repression.was more frequently observed in citric acid fermentations (Shu and Johnson 1948, Gupta et al 1976, Kristiansen and Sinclair 1978, Dawson et al 1988, Xu et al 1989b). …”

Section: Effect Of Feeding With Nitrogen and /Or Phosphoroussupporting

confidence: 52%

See 1 more Smart Citation

Citric acid production byAspergillus foetidusin batch and fed‐batch cultures

Chen

1993

Food Biotechnology

View full text Add to dashboard Cite

Sucrose and ammonium nitrate, respectively, were the preferred carbon and nitrogen sources for citric acid production by Aspergillis foetidus CCRC 30206. For a 7-day fermentation, the maximal citric acid production of 7.2 ~ 28.4 g/l appeared at an initial sucrose concentration of 12 % under various initial levels of nitrogen and phosphorous sources. Citric acid production commenced and ceased earlier in either the nitrogen-limited (NH 4 NO 3 , 0.5g/l) or the phosphorous-limited (KH 2 PO 4 , 0.2 g/1) medium.The richer media delayed the onset of citric acid production. Nonetheless, 48.3 g/1 of citric acid was produced in the medium rich in nitrogen (NH 4 NO 3 , 2.0 g/1) and phosphorous (KH 2 PO 4 , 1.4 g/1) when the batch culture was extended to 16 days. The cultivation time of this rich medium was reduced to 12 days in a fed-batch culture under dual limitation of both nitrogen and phosphorous.

show abstract

Section: Effect Of Cultivation Termscontrasting

confidence: 79%

Section: Effect Of Feeding With Nitrogen and /Or Phosphoroussupporting

confidence: 52%

Citric acid production byAspergillus foetidusin batch and fed‐batch cultures

Chen

1993

Food Biotechnology

View full text Add to dashboard Cite

show abstract

“…In the continuous fermentor (chemostat), low dilution rates and high levels of dissolved oxygen increased citric acid productivities. Using this information, Dawson et al 4 developed a fed-batch fermentor with an overall productivity twice that obtained in a traditional batch fermentor. In yeast, fed-batch could be used for maintaining low nitrogen levels in the fermentor, thereby promoting the production of citric acid as well as maintaining cell viability.…”

Section: Introductionmentioning

confidence: 96%

Citric acid production by Candida lipolytica Y 1095 in cell recycle and fed‐batch fermentors

Rane

Sims

1995

Biotech & Bioengineering

View full text Add to dashboard Cite

The effect of dissolved oxygen on citric acid production and oxygen uptake by Candida lipolytica Y 1095 was evaluated in cell recycle and fed-batch fermentation systems. The maximum observed volumetric productivity, which occurred at a dilution rate of 0.06 h(-1), a dissolved oxygen concentration of 80%, and a biomass concentration of 5% w/v, in the cell recycle system, was 1.32 g citric acid/L . h. At these same conditions, the citric acid yield was 0.65 g/g and the specific citric acid productivity was 24.9 mg citric acid/g cell . h. In the cell recycle system, citric acid yields ranged from 0.45 to 0.72 g/g. Both the volumetric and specific citric acid productivities were dependent on the dilution rate and the concentration of dissolved oxygen in the fermentor. Similar productivities (1.29 g citric acid/L . h) were obtained in the fed-batch system operated at a cycle time of 36 h, a dissolved oxygen concentration of 80%, and 60 g total biomass. Citric acid yields in the fed-batch fermentor were consistently lower than those obtained in the cell recycle system and ranged from 0.40 to 0.59 g/g. Although citric acid yields in the fed-batch fermentor were lower than those obtained in the cell recycle system, higher citric:isocitric acid ratios were obtained in the fed-batch fermentor. As in the cell recycle system, both the volumetric and specific citric acid productivities in the fed-batch fermentor were dependent on the cycle time and dissolved oxygen concentration. (c) 1995 John Wiley & Sons, Inc.

show abstract

“…Despite this setback using whey as the substrate, research continued using a sucrose substrate, and a fed-batch fermentation process was developed based on continuous feeding of the nitrogen source. Using this technique, the growth rate of A. niger was controlled and maintained at the optimum value for citrate production (Dawson et al, 1988). The importance of maintaining a high dissolved oxygen tension was also demonstrated.…”

Section: B Citric Acidmentioning

confidence: 99%

Biotechnological Developments in New Zealand

Maddox

Gutierrez

1996

Critical Reviews in Biotechnology

View full text Add to dashboard Cite

Biotechnology has a long history in New Zealand, and there have been some innovations that are unique to the country. This article describes some developments that illustrate this point and gives some examples of current research projects that typify the strengths and interests of the biotechnology community. Examples are taken from the food industry, agriculture, forestry, production of industrial chemicals, and the treatment of hazardous chemicals.

show abstract

Application of fed‐batch culture to citric acid production by Aspergillus niger: The effects of dilution rate and dissolved oxygen tension

Cited by 42 publications

References 12 publications

Citric acid production byAspergillus foetidusin batch and fed‐batch cultures

Citric acid production byAspergillus foetidusin batch and fed‐batch cultures

Citric acid production by Candida lipolytica Y 1095 in cell recycle and fed‐batch fermentors

Biotechnological Developments in New Zealand

Contact Info

Product

Resources

About