Application of External Force Regulates the Migration and Differentiation of Adipose-Derived Stem/Progenitor Cells by Altering Tissue Stiffness

Li, Ye; Wu, Mengfan; Zhang, Ziang; Xia, Jing; Wang, Zijue; Chen, Xinyao; Xiao, Xiuyun; Lu, Feng; Dong, Ziqing

doi:10.1089/ten.tea.2019.0046

Cited by 20 publications

(19 citation statements)

References 39 publications

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“…However, large negative pressure reduced adipogenic differentiation and increased fibrosis in adipose tissue in an EPAT rat model. 63 EVE did not promote the differentiation of ADSCs, 32 and cyclic stretching alone did not increase in vitro adipogenesis of ADSCs. 84 The discrepancies among those findings may be due to the differences in types and levels of mechanical strain applied to cells.…”

Section: Eve-induced Adipogenesis In Adipose Tissuesmentioning

confidence: 77%

“…Finite element analysis was performed to simulate the force exerted by the EVE device and its effect on stiffness of EPAT. 63 Adipose tissue stiffness increased upon exposure to external force, decreased when this force was removed, and increased again when this force was reapplied. Environmental stiffness is thought to influence cell activities; for example, cells may migrate from areas of low to high stiffness.…”

Section: Eve-induced Cell Migration In Adipose Tissuesmentioning

confidence: 96%

“…66 To investigate the migration and number of ADSCs, these cells were transplanted into the EPAT rat model. 63 The external force generated by an EVE device increased the stiffness of adipose tissue, with these mechanical stimuli transmitted from the ECM to the cytoskeleton through internal components such as actin filaments, myosin motors, and actin cross-linking proteins. 67 Therefore, increased stiffness can result in upregulation of the expression of cytoskeleton-related proteins, including CDC42, RhoA, and Dynamin, and lead to the migration of ADSCs (Fig.…”

Section: Eve-induced Cell Migration In Adipose Tissuesmentioning

confidence: 99%

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The Application and Mechanism of Action of External Volume Expansion in Soft Tissue Regeneration

Chen

Yuan

2021

Tissue Engineering Part B: Reviews

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The reconstruction of large-volume soft tissue defects is a major problem in plastic surgery. Many plastic surgeons have focused on external volume expansion (EVE) because of its capacity to promote regeneration of soft tissues, including breast, subcutaneous fat, and skin. EVE is a minimally invasive and less costly tissue engineering approach that has shown great clinical potential. However, many challenges still need to be addressed before such technology can become a common clinical practice. Basic in vivo and in vitro studies have been performed to determine the possible mechanisms by which EVE promotes tissue regeneration and to design optimized animal models. EVE application was found to facilitate cell proliferation and migration, enhance adipogenesis, improve angiogenesis, and provide available space for soft tissue growth. Understanding the mechanical and chemical signals associated with EVE during tissue regeneration may enable the clinical adaptation of this technology. This article reviews the clinical application of EVE techniques, describes preclinical animal models, and evaluates the possible mechanisms by which EVE induces tissue regeneration.

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Section: Eve-induced Adipogenesis In Adipose Tissuesmentioning

confidence: 77%

Section: Eve-induced Cell Migration In Adipose Tissuesmentioning

confidence: 96%

Section: Eve-induced Cell Migration In Adipose Tissuesmentioning

confidence: 99%

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The Application and Mechanism of Action of External Volume Expansion in Soft Tissue Regeneration

Chen

Yuan

2021

Tissue Engineering Part B: Reviews

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“…Based on our ndings, it seems reasonable to conclude that in a clinical context, it is important to pay attention to the concentrations of ASCs in fat grafts. A suitable concentration of ASCs could decrease brosis and increase long-term retention, whereas excessive ASCs could have the opposite effects [51,52]. Since ASCs can directly induce the phenotype of M2 macrophage, the transformation of macrophages may also affect the shifting process of CD4 + T-cells [6,53].…”

Section: Discussionmentioning

confidence: 99%

Adipose-derived stem cells regulate fibrosis by altering CD4+ T-cell immune responses in fat grafting in a mouse model

Chen

Wang

et al. 2020

Preprint

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Background Autologous fat grafting is becoming increasingly common worldly. However, the long-term retention of fat grafting is still unpredictable due to the inevitable fibrosis that arises during tissue repair. Fibrosis may be regulated by T-cell immune responses that are influenced by adipose-derived stem cells (ASCs). Accordingly, we hypothesized that overly abundant ASCs might promote fibrosis by promoting T-cell immune responses to adipose tissue. Methods We performed 0.3 ml fat grafts with 104/ml, 106/ml and 108/ml ASCs and control group in C57 BL/6 mice in vivo. We observed retention, fibrosis, T-cell immunity, and macrophage infiltration over 12 weeks. In addition, CD4 + T-helper 1 (Th1) cells and T-helper 2 (Th2) cells were co-cultured with ASCs or ASCs conditioned media (CM) in vitro. We detected the ratio of Th2%/Th1% after 24 and 48 hours. Results In vivo, the retention rate was higher in the 104 group, while even lower in the 108 group with significantly increased inflammation and fibrosis than the control group at week 12. There was no significance between control group and the 106 group. Also, the 108 group increased infiltration of M2 macrophages, CD4 + T-cells and Th2/Th1 ratio. In vitro, the ratio of Th2%/Th1% induced by the ASCs-transwell group was higher than the ASCs-CM group and showed concentration-dependent. Conclusions High concentrations of ASCs in adipose tissue can promote Th1–Th2 shifting, and the excess of Th2 cells might promote the persistence of M2 macrophages and increase the level of fibrosis which lead to a decrease in the long-term retention of fat grafts. In addition, we found that ASCs promoted Th1–Th2 shifting in vitro.

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“…Accumulating evidence indicates that activating cells through mechanical signals in the extracellular microenvironment can mediate a series of signaling pathways through cytoskeleton rearrangement, thereby altering the proliferation and differentiation abilities of progenitor cells (Nava et al, 2012;Sun et al, 2012;Li Y. et al, 2019;Chen et al, 2020;Zhang et al, 2020). An SVF/ECM gel is a type of Coleman fat obtained using standard Coleman technology following centrifugation and is further mechanically isolated.…”

Section: Introductionmentioning

confidence: 99%

Phenotypic and Cellular Characteristics of a Stromal Vascular Fraction/Extracellular Matrix Gel Prepared Using Mechanical Shear Force on Human Fat

Yuan

Zou

Tan

et al. 2021

Front. Bioeng. Biotechnol.

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The retention of fat-derived grafts remains a challenge for regenerative medicine. Fat aspirates from patients undergoing liposuction were prepared into standard Coleman fat grafts or further isolated using mechanical shear force to prepare a stromal vascular fraction (SVF)/extracellular matrix (ECM) gel. The retention rate of the SVF/ECM gel was significantly higher than that of the Coleman fat at 3, 14, 28, and 60 days following transplantation on the backs of nude mice. The viscosity of the fat was directly proportional to the shearing force. Although the mechanical isolation did not affect the total number of cells, it significantly decreased the number of living cells. Flow cytometry showed a greater number of mesenchymal stem cells, supra-adventitial (SA)-adipose stromal cells (ASCs), and adipose-derived stem cells but a lower number of endothelial progenitor cells in the SVF/ECM gel than in the Coleman fat. Thus, mechanical isolation of fat can increase the pluripotency of adipocytes, which can improve graft retention in cell therapy.

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Application of External Force Regulates the Migration and Differentiation of Adipose-Derived Stem/Progenitor Cells by Altering Tissue Stiffness

Cited by 20 publications

References 39 publications

The Application and Mechanism of Action of External Volume Expansion in Soft Tissue Regeneration

The Application and Mechanism of Action of External Volume Expansion in Soft Tissue Regeneration

Adipose-derived stem cells regulate fibrosis by altering CD4+ T-cell immune responses in fat grafting in a mouse model

Phenotypic and Cellular Characteristics of a Stromal Vascular Fraction/Extracellular Matrix Gel Prepared Using Mechanical Shear Force on Human Fat

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