Application of EPR to porphyrin-protein agents for photodynamic therapy

Sannikova, Natalya; Timofeev, Ivan O.; Chubarov, Alexey S.; Lebedevа, N. Sh.; Семейкин, А. С.; Kirilyuk, Igor A.; Tsentalovich, Yuri P.; Fedin, Matvey V.; Bagryanskaya, Elena G.; Krumkacheva, Olesya A.

doi:10.1016/j.jphotobiol.2020.112008

Cited by 22 publications

(21 citation statements)

References 51 publications

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“…However, this method introduces the albumin one residue per molecule. Usually, the procedure is used for fluorescence dye for the cell experiments [84], fluorescence imaging [38,85] applications, or spin-label conjugation for the protein complexes investigation [62,[86][87][88]. For drug delivery, another method is required for better therapeutic effect, which possesses multi-conjugation.…”

Section: Covalent Strategymentioning

confidence: 99%

Serum Albumin for Magnetic Nanoparticles Coating

Chubarov

2022

Magnetochemistry

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Magnetic nanoparticles (MNPs) have great potential in biochemistry and medical science. In particular, iron oxide nanoparticles have demonstrated a promising effect in various biomedical applications due to their high magnetic properties, large surface area, stability, and easy functionalization. However, colloidal stability, biocompatibility, and potential toxicity of MNPs in physiological environments are crucial for their in vivo application. In this context, many research articles focused on the possible procedures for MNPs coating to improve their physic-chemical and biological properties. This review highlights one viable fabrication strategy of biocompatible iron oxide nanoparticles using human serum albumin (HSA). HSA is mainly a transport protein with many functions in various fundamental processes. As it is one of the most abundant plasma proteins, not a single drug in the blood passes without its strength test. It influences the stability, pharmacokinetics, and biodistribution of different drug-delivery systems by binding or forming its protein corona on the surface. The development of albumin-based drug carriers is gaining increasing importance in the targeted delivery of cancer therapy. Considering this, HSA is a highly potential candidate for nanoparticles coating and theranostics area and can provide biocompatibility, prolonged blood circulation, and possibly resolve the drug-resistance cancer problem.

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Section: Covalent Strategymentioning

confidence: 99%

Serum Albumin for Magnetic Nanoparticles Coating

Chubarov

2022

Magnetochemistry

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“…Sterically-hindered nitroxides have much slower rates of reduction and recently were shown for such "unusual" applications as MRI [56,[69][70][71][72][73]. Nitroxide spin-labels have been classically used for two spin-labeled molecules complex investigations (distance measurement) by pulsed dipolar spectroscopy (PDS) methods of electron paramagnetic resonance (EPR), PELDOR (also known as DEER) method [43,[74][75][76][77][78].…”

Section: Homocysteine Thiolactone Building Blocks As Potential Precursors For Materials and Probesmentioning

confidence: 99%

Homocysteine Thiolactone: Biology and Chemistry

Chubarov

2021

Encyclopedia

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Homocysteine thiolactone is a five-membered cyclic thioester of amino acid homocysteine. It is generated from homocysteine as a result of an error-editing reaction, principally, of methionyl-tRNA synthetase. An elevated level of homocysteine thiolactone is associated with cardiovascular diseases, strokes, atherosclerosis, neurological abnormalities, etc., presumably because it reacts to the side chain of protein lysine causing protein damage and autoimmune responses. It is not only an important metabolite but also a versatile building block for organic and bioorganic synthesis. This entry contains data on the homocysteine thiolactone formation, metabolism, toxicity mechanism in vivo, and the bioorganic chemistry applications as a powerful synthetic tool in polymer science, sustainable materials development, and probes.

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“…16,17 The first work demonstrating the capabilities of dipolar EPR with photoexcited triplet labels was carried out using porphyrins, 16 and several other studies have followed in a few years. [18][19][20][21][22][23][24][25][26][27] The main advantage of porphyrins is their good water solubility, which allows spin labeling 16,20 or complexation with proteins. 23 The first application of triplet porphyrins was demonstrated for the 4-pulse DEER experiment, where porphyrins were photoexcited shortly before the microwave pulse sequence and used as an observer (laser-induced (Li) DEER).…”

Section: Introductionmentioning

confidence: 99%

“…[18][19][20][21][22][23][24][25][26][27] The main advantage of porphyrins is their good water solubility, which allows spin labeling 16,20 or complexation with proteins. 23 The first application of triplet porphyrins was demonstrated for the 4-pulse DEER experiment, where porphyrins were photoexcited shortly before the microwave pulse sequence and used as an observer (laser-induced (Li) DEER). 16 Strong triplet hyperpolarization in LiDEER led to drastically enhanced sensitivity compared to common DEER with the nitroxide observer.…”

Section: Introductionmentioning

confidence: 99%