Application of enzyme multibiosensor for toxicity analysis of real water samples of different origin

Abstract

Aim. The analysis of toxicity of different water samples with the multibiosensor developed earlier. Methods. The potentiometric multibiosensor with several immobilized enzymes as bioselective elements and the matrix of pH-sensitive field effect transistors as transducers of the biochemical signal into the electric one was applied for the analysis. Results. The bioselective elements of the multibiosensor were developed using acetylcholinesterase, butyryl- cholinesterase, urease, glucose oxidase, and three-enzyme system (invertase, mutarotase, glucose oxidase). The measurement of toxic compounds in water samples of different origin was performed using the constructed sensor. The results obtained were compared with those obtained by the conventional methods of toxic agent’s analysis (atomic absorption spectrometry, thin-film chroma- tography, and atomic absorbic analyser of mercury). Conclusion. A strong conformity between the results obtained with the multibiosensor and traditional methods has been shown

Biosensors. A quarter of a century of R&D experience

“…N o t e s. AAAHg 2+ -atomic absorption mercury analyzer; AAS -atomic absorption spectroscopy; TLC -thin layer chromatography; «+»exceeding MPC; «++» -exceeding MPC by several orders of magnitude. Table 3 Comparison of different methods of the analysis of real samples of the environment [43] tylcholine, butyrylcholine, penicillin, formaldehyde, arginine, etc. (Table 4).…”

“…We were the first to propose the conception of toxins determination by a multibiosensor based on the enzyme inhibition analysis [10]. The multibiosensor with a matrix of ISFETs and several bioselective elements (AcChE, BuChE, urease, glucose oxidase, and three-enzyme system -invertase, mutarotase, glucose oxidase) was developed and its main charateristics were examined [40][41][42][43]. The results of inhibition of the enzyme system by different concentrations of individual toxicants and their mixtures were analyzed by the methods of mathematical statistics to develop the approach to quantitative or semi-quantitative determination of toxicants in real water samples of the environment [42].…”

“…On the basis of this multibiosensor, we created a portable laboratory device to analyze real water samples from a number of Kyiv water reservoirs, and complex multicomponent water assays from the landfill waste for the presence of toxic substances [43]. A comparison of the sensor data with traditional methods shows a high degree of correlation (Table 3) [43].…”

Flow-Through Acetylcholinesterase Sensor with Replaceable Enzyme Reactor

“…Starting from chemical warfare detection [ 12 , 13 ], AChE biosensors have found a large application in the determination of organophosphate and carbamate pesticides inhibiting enzymes [ 14 , 15 , 16 ]. Due to a wide variety of species exerting the anticholinesterase effect, AChE biosensors were proposed as indicators of general pollution of the environment [ 17 ] and for detection of heavy metals [ 18 ], surfactants [ 19 ], fluorides [ 20 ] and organic solvents [ 21 , 22 ] exerting a non-specific inhibitory effect. Recently, AChE biosensors were also successfully utilized for the determination of reversible inhibitors, e.g., anti-dementia drugs [ 23 , 24 ] and aflatoxins [ 25 , 26 ].…”

New Trends in Biosensors for Water Monitoring

“…Multianalyte devices combining informations from several different types of enzymes have been also proposed. For example, Soldatkin et al recently developped an amperometric multibiosensor using the inhibition of acetylcholinesterase, butyryl-cholinesterase, urease, glucose oxidase, and three-enzyme system (invertase, mutarotase, glucose oxidase) for water toxicity assessment (Soldatkin et al, 2009). …”