Application of enzyme-linked immunosorbent assay for epidemiological studies of diseases of livestock in the tropics of Mexico

Ja, Barajas-Rojas; Hp, Riemann; Ce, Franti

doi:10.20506/rst.12.3.723

Cited by 8 publications

(5 citation statements)

References 7 publications

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“…The detection of anti‐M bovis antibodies in blood samples might prove to be a quick and inexpensive screening method for epidemiological studies. In the 1980s, several experimental ELISAS were developed (Boothby and others 1981, Howard and others 1982, Hajkova and Jurmanova 1986, Thomas and others 1987, Uhaa and others 1990, Barajas‐Rojas and others 1993, Liberal and Boughton 1994) when the antigenic variability and genomic heterogeneity of strains of M bovis were poorly understood. Recent studies have proved that a single M bovis strain can exhibit multiple antigenic membrane configurations (Poumarat and others 1994, Rosengarten and others 1994) which can greatly affect the reliability of the screening and diagnostic tools (Rosengarten andYogev 1996).…”

Section: Methodsmentioning

confidence: 99%

Serological prevalence of Mycoplasma bovis infection in suckling beef cattle in France

Grand

Bezille

Calavas³

et al. 2002

Veterinary Record

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The prevalence of Mycoplasma bovis infection in France was assessed by means of a serological survey of suckling beef cattle, using an ELISA. The survey included 824 randomly selected herds in eight French counties and a total of 32,197 animals more than one year old. In each county, the number of herds tested was determined statistically on the basis of the hypothesis that about 40 per cent of herds are infected. The proportion of herds containing at least one infected animal ranged from 28 to 90 per cent depending on the county. Among the 32,197 sera tested, the animal infection rate ranged between 2 per cent and 13 per cent. In infected herds, the average number of positive animals per herd was between 10 and 20 per cent, and the infection was unevenly distributed among the areas tested.

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Section: Methodsmentioning

confidence: 99%

Serological prevalence of Mycoplasma bovis infection in suckling beef cattle in France

Grand

Bezille

Calavas³

et al. 2002

Veterinary Record

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“…To determine animal seroconversion due to Babesia sp. and A. marginale infection, serum samples were obtained from the experimental cattle and were analyzed by an Indirect Fluorescent Antibody Test (IFAT), 13 and an ELISA test, 14 respectively.…”

Section: Methodsmentioning

confidence: 99%

Bovine Babesiosis and Anaplasmosis Follow‐up on Cattle Relocated in an Endemic Area for Hemoparasitic Diseases^a

Millán

Alvarez

Ramos

et al. 1998

Annals of the New York Academy of Sciences

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A multiplex PCR/DNA probe assay was used to monitor Babesia bovis, B. bigemina and Anaplasma marginale infection in cattle introduced to a Boophilus microplus-infested area in Veracruz, Mexico. Eight intact, 18-month-old, cross-bred beef cattle (four naive, Group A; four Babesia species--premunized, Group B) were immediately exposed to ticks after arrival and were clinically monitored from day 6 to day 98 post-exposure (PE) to ticks. Blood sample analysis for DNA detection by the MPCR/DNA probe assay showed that Group A animals were infected with B. bovis from day 11 up to day 22 PE, requiring treatment on days 17-20. Group B animals were detected positive to B. bovis on days 17-20, did not require treatment and remained persistently infected from days 70 to 84 PE. Treatment of Group A animals delayed the infection with B. bigemina. These animals became positive to the parasite on days 63-77 PE. In contrast, Group B animals (untreated) showed B. bigemina infection on days 21-26 and 63-84 PE. One animal was positive for A. marginale infection on days 63-66 PE, the rest of the animals became so on days 80-98 PE. All infected animals required treatment with oxytetracycline. Monitoring the triple hemoparasite infection with the MPCR/DNA probe assay provided important epidemiological information. Thus, precautionary measures can be established when cattle are moved to a babesiosis/anaplasmosis risk area.

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“…El tamaño de muestra se calculó mediante la fórmula: TM= (TP/2. 25) x (Nc-Fe), donde: TM= tamaño de muestra, TP= tamaño de la población estudiada, 2.25= constante, Nc= nivel de confianza para inferir sobre la población, y Fe= frecuencia esperada (15) . Esta fórmula se adaptó considerando que la población en este caso fue de tamaño conocido (100 vientres por hato), con una frecuencia esperada de 0.50 y un nivel de confianza del 0.95 (P<0.05).…”

Section: Recibido 19/08/2017unclassified

“…Cantú y Alvarado (9) Tamaulipas Aldama No se indica No se indica 34.1 Cantú y Alvarado (9) Tamaulipas González No se indica No se indica 63.8 Cantú y Alvarado (9) Tamaulipas Altamira No se indica No se indica 52.1 Cantú y Alvarado (9) Tamaulipas Promedio No se indica 500 49.0 Solorio (10) Michoacán Tarímbaro H 961 63.2 Córdova-Izquierdo et al (11) Campeche Candelaria Bt, Bi, Bt x Bi 267 12.3 Segura-Correa et al (12) Nuevo León No se indica No se indica 813 30.0 Ramos et al (13) Oaxaca San Juan Cotzocón No se indica 1031 41.0 Romero et al (17) Veracruz Norte No se indica Múltiples razas y cruzas 804 64.2 Romero et al (17) Veracruz Centro No se indica Múltiples razas y cruzas 756 57.4 Romero et al (17) Veracruz Sur No se indica Múltiples razas y cruzas 574 59.3 Romero et al (17) Promedio No se indica Múltiples razas y cruzas 3,538 60.3 Cedillo et al (18) Coahuila Torreón H 116 83.6 Escamilla et al (19) Querétaro Colón Holstein 99 70.0 Mellado (23) Coahuila Saltillo CH, HF 19 100.0 Barajas-Rojas et al (25) Veracruz Tlapacoyan H x C 865 <5.0 Meléndez et al (26) Aguascalientes No se indica H 110 32.8 Ojeda-Carrasco et al (27) Estado de México Tlalmanalco No se indica 40 40.0 Ojeda-Carrasco et al (27) Estado de México Amecameca No se indica 91 58.2 Ojeda-Carrasco et al (27) Estado de México Ayapango No se indica 47 29.8 Ojeda-Carrasco et al (27) Promedio -No se indica 178 42.7 Córdova-Izquierdo et al (28) Sureste mexicano No se indica L, CH, BB, BM, SP x C 69 87.0 Moles et al (29) Altiplano Central No se indica No se indica 603 72.3 Sánchez-Castilleja et al (30) Hidalgo No se indica H 500 48.6 Solís-Calderón et al (31) Yucatán No se indica C, H x C, SP x C 560 14.0 Varguez et al (32) Campeche Sur b No se indica No se indica 46.4 Varguez et al (32) Campeche Centro (32) Campeche Norte (32) Promedio En el municipio de Nauzontla la prevalencia disminuyó 23.1 unidades porcentuales del primero al segundo muestreo, mientras que en el de San José Acateno la prevalencia disminuyó 23.9 ...…”

Section: Recibido 19/08/2017unclassified

Prevalencia de anticuerpos contra diarrea viral bovina en vacas no vacunadas en los estados de Puebla, Tabasco y Veracruz, México

Fernández¹,

Utrera²,

Martínez³

et al. 2018

Rev. Mex. Cienc. Pecu.

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La prevalencia (PRE) de anticuerpos contra diarrea viral bovina (DVB) se determinó en 421 vacas de 24 ranchos ubicados en 11 municipios de los estados de Puebla, Veracruz y Tabasco, México. Adicionalmente, se evaluó la influencia de anticuerpos contra DVB sobre la tasa de gestación (TG). Antes del estudio, los hatos nunca fueron vacunados contra DVB. Se obtuvieron muestras de sangre de cada vaca dos veces, con un intervalo de 3.5 a 4.0 meses. La presencia de anticuerpos se determinó mediante la técnica de ELISA. Al primer muestreo, Puebla (51.4 %) y Tabasco (49.7 %) presentaron menor PRE (P<0.01) que Veracruz (76.2 %); sin embargo, al segundo muestreo, los tres estados presentaron diferentes (P<0.05) PRE: 31.1, 54.6 y 76.0 %, respectivamente. Al primer muestreo, la PRE varió de 25.0 a 85.0 % entre municipios, mientras que al segundo varió de 15.0 a 85.9 %. Al primer muestreo, la PRE varió de 9.1 a 95.0 % entre ranchos, mientras que al segundo varió de 5.0 a 95.0 %. La TG de las vacas sin anticuerpos contra DVB fue similar (P>0.05) a la de las vacas con anticuerpos (57.3 vs 53.5 %). En conclusión, 1) Veracruz presentó mayor PRE que Puebla y Tabasco; 2) el 100 % de los hatos presentaron anticuerpos contra DVB, lo que sugiere que el virus de la DVB está ampliamente distribuido en los tres estados; 3) existió una gran variación en la PRE entre ranchos y entre municipios, siendo ésta cercana a 100 % en varios casos; y 4) la presencia de anticuerpos contra DVB no influyó la tasa de gestación.

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Application of enzyme-linked immunosorbent assay for epidemiological studies of diseases of livestock in the tropics of Mexico

Cited by 8 publications

References 7 publications

Serological prevalence of Mycoplasma bovis infection in suckling beef cattle in France

Serological prevalence of Mycoplasma bovis infection in suckling beef cattle in France

Bovine Babesiosis and Anaplasmosis Follow‐up on Cattle Relocated in an Endemic Area for Hemoparasitic Diseases^a

Prevalencia de anticuerpos contra diarrea viral bovina en vacas no vacunadas en los estados de Puebla, Tabasco y Veracruz, México

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Application of enzyme-linked immunosorbent assay for epidemiological studies of diseases of livestock in the tropics of Mexico

Cited by 8 publications

References 7 publications

Serological prevalence of Mycoplasma bovis infection in suckling beef cattle in France

Serological prevalence of Mycoplasma bovis infection in suckling beef cattle in France

Bovine Babesiosis and Anaplasmosis Follow‐up on Cattle Relocated in an Endemic Area for Hemoparasitic Diseasesa

Prevalencia de anticuerpos contra diarrea viral bovina en vacas no vacunadas en los estados de Puebla, Tabasco y Veracruz, México

Contact Info

Product

Resources

About

Bovine Babesiosis and Anaplasmosis Follow‐up on Cattle Relocated in an Endemic Area for Hemoparasitic Diseases^a