Application of Enzyme Immunoassay Kits for the Determination of Pig Plasma Progesterone Levels.

Kamei, Masahiro; Iwamura, Shohkichi; Yoshioka, K; Kamomae, Hideo

doi:10.5938/youton.31.109

Cited by 1 publication

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The concentration of P in the serum was determined by enzyme immunoassay (EIA) according to the procedure used by Kamei et al [16]. In brief, 0.05 ml of blood plasma was extracted with ether, dried, and reconstituted in 500 µl of 0.05% bovine serum albumin in phosphate buffered saline (BSA-PBS).…”

Section: Blood Samples and Hormone Assaysmentioning

confidence: 99%

Induction of Estrus by Prostaglandin F2.ALPHA. Administration in the Vaginal Vestibules of Miniature Pigs

Kuge

Iwata

Kuwayama

et al. 2006

Journal of Reproduction and Development

View full text Add to dashboard Cite

Abstract. Estrus induction is an important step in embryo production. It has been difficult to induce estrus in miniature pigs by intramascular (i.m.) injection of prostaglandin F2α (PGF22α) in the early luteal stage of the estrous cycle. In the present study, we injected two different doses of PGF22α i.m. and into the submucosa of the vaginal vestibule (i.ves.) of miniature pigs, and examined the effect of these treatments on estrus induction. Fifteen miniature pigs were divided into five experimental groups (control, saline injected i.m.; PGF2α treated, 1.0 or 1.5 mg of PGF2α injected twice i.m. or i.ves.), and the estrus length and concentrations of 17β-estradiol (E2) and progesterone (P) in the blood were examined. Estrus length was significantly shortened by a large amount of PGF2α injected i.ves. In addition, the concentration of P in the blood significantly decreased after two injections of PGF2α (i.m. or i.ves.). These results suggest that in miniature pigs, administration of at least 3.0 mg of PGF2α is required for the induction of luteolysis and injection of PGF2α into the vaginal vestibule is a useful method of estrus induction.

show abstract

Section: Blood Samples and Hormone Assaysmentioning

confidence: 99%