Application of Enzymatic-Based Bioreactors

Co-Immobilization and Compartmentalization of Cholesterol Oxidase, Glucose Oxidase and Horseradish Peroxidase for Improved Thermal and H2o2 Stability

“…Such lowered competition is a consequence of both the configuration of the reactor and the application of pressure, which enables flow of substrates and products from top to bottom. Despite lower productivity, the systems involving immobilized enzymes allow for better control of process conditions and set-up versatility, such as use of fed-batch mode or continuous mode [21]. Moreover, such biosystems can retain higher catalytic activity under high temperature conditions and at the presence of higher concentration of H 2 O 2.…”

“…For example, the most suitable membrane for attachment of specific enzymes may be chosen based on molecular weight cut-off of the membrane, hydrophobicity/hydrophilicity, and the presence of specific functional groups on the membrane surface, which besides membrane pore size affect the efficiency of biomolecule immobilization [16][17][18]. Furthermore, the greatest advantage of using membranes as supports for enzyme immobilization is the possibility of conducting enzymatic membrane reactor experiments with advanced control of process conditions and set-up versatility, such as fed-batch mode or continuous mode [19][20][21]. Electrospun fibers possess similar properties to commercially available membranes.…”

Co-Immobilization and Compartmentalization of Cholesterol Oxidase, Glucose Oxidase and Horseradish Peroxidase for Improved Thermal and H2o2 Stability

“…Such lowered competition is a consequence of both the configuration of the reactor and the application of pressure, which enables flow of substrates and products from top to bottom. Despite lower productivity, the systems involving immobilized enzymes allow for better control of process conditions and set-up versatility, such as use of fed-batch mode or continuous mode [21]. Moreover, such biosystems can retain higher catalytic activity under high temperature conditions and at the presence of higher concentration of H 2 O 2.…”

“…For example, the most suitable membrane for attachment of specific enzymes may be chosen based on molecular weight cut-off of the membrane, hydrophobicity/hydrophilicity, and the presence of specific functional groups on the membrane surface, which besides membrane pore size affect the efficiency of biomolecule immobilization [16][17][18]. Furthermore, the greatest advantage of using membranes as supports for enzyme immobilization is the possibility of conducting enzymatic membrane reactor experiments with advanced control of process conditions and set-up versatility, such as fed-batch mode or continuous mode [19][20][21]. Electrospun fibers possess similar properties to commercially available membranes.…”