Application of Endo-β-1,4,d-mannanase and Cellulase for the Release of Mannooligosaccharides from Steam-Pretreated Spent Coffee Ground

Abstract

Spent coffee ground (SCG), a present waste stream from instant coffee production, represents a potential feedstock for mannooligosaccharides (MOS) production. MOS can be used in nutraceutical products for humans/animals or added to instant coffee, increasing process yield and improving product health properties. The SCG was evaluated for MOS production by steam pretreatment and enzymatic hydrolysis with a recombinant mannanase and a commercial cellulase cocktail (Acremonium, Bioshigen Co. Ltd, Japan). The mannanase was produced using a recombinant strain of Yarrowia lipolytica, used to produce and secrete endo-1,4-β,D-mannanase from Aspergillus aculeatus in bioreactor cultures. Endo-1,4-β,D-mannanase was produced with an activity of 183.5 U/mL and 0.23 mg protein/mL. The enzyme had an optimum temperature of 80 °C, and the activity in the supernatant was improved by 150 % by supplementation with 0.2 % sodium benzoate and 35 % sorbitol as a preservative and stabiliser, respectively. The steam pretreatment of SCG improved the enzymatic digestibility of SCG, thus reducing the required enzyme dosage for MOS release. Combined enzymatic hydrolysis of untreated or steam-pretreated (150, 190 and 200 °C for 10 min) SCG with mannanase and cellulase cocktail resulted in 36-57 % (based on mannan content) of MOS production with a degree of polymerization of up to 6. The untreated material required at least 1 % of both mannanase and cellulase loading. The optimum mannanase and cellulase loadings for pretreated SCG hydrolysis were between 0.3 and 1 and 0.4 and 0.8 %, respectively. Statistical analysis suggested additive effect between cellulase cocktail and mannanase on MOS release, with no indication of synergism observed.

Novel β-1,4-Mannanase Belonging to a New Glycoside Hydrolase Family in Aspergillus nidulans

“…The diverse industrial applications of β-mannanases include de-inking paper waste, clarifying fruit juices, upgrading feed, fodder, and fibers, and saccharifying plant biomass. The novel β-mannanase described herein should be of interest to the food and pulp industries and to studies of the conversion technology of lignocellulosic biomass ( 39 ).…”

Diversity and Bioprospecting of Filamentous Fungi Isolated From Nausitora Fusticulus (Bivalvia: Teredinidae) Digestive Organs for Lignocellulolytic Enzymes / Diversidade E Bioprospecção De Fungos Filamentosos Isolados Dos Órgãos Digestivos De Nausitora Fusticulus (Bivalvia:teredinidae) Para Obtenção De Enzimas Lignocelulolíticas

“…A robust enzymatic cocktail is fundamentals for application such as biomass conversion into high added products (Chiyanzu et al, 2014). Moreover, combination of different enzymes is positive, improving the cocktail application allowing for example hemicellulases enzymes uses (Freitas et al, 2020;Bueno et al, 2020;Calore et al, 2020).…”

Extraction of oligosaccharides and phenolic compounds by roasting pretreatment and enzymatic hydrolysis from spent coffee ground

“…The acid and alkali agents have been used, such as diluted sulfuric acid [1], potassium hydroxide [12], and sodium hydroxide [13,14]. Several other polysaccharides extraction methods were reported, such as steam explosion [15], using the subcritical water [16] and enzymatic hydrolysis [3].…”