Application of ELISA-ABC method to the identification of minute human bloodstains

Abstract

Bloodstained threads (1 cm in length) were tested to identify human origin by a direct ELISA-ABC method using biotinylated antibody against human HbA0. By this method human bloodstains were clearly distinguishable from bloodstains of other species including Japanese monkey. The minimum detection limit of bloodstains prepared from undiluted human whole blood was 1:5, 120 (28 ng Hb) and that of bloodstains from diluted human whole blood was 1:640-1:1, 280. Human Hb was more easily detectable in bloodstains prepared from diluted human blood after extraction with 5% ammonia than after extraction with phosphate-buffered saline.

A polymerase chain reaction‐based method for the identification of DNA samples from common vertebrate species

“…The bands were markedly discrete and scorable, Table 1. Lane (1) 100 bp ladder marker (1 yg total; the three most intense bands correspond to 600 bp, 1500 bp, and 2072 bp (uncut vector); (2) rainbow trout (Salmo gairdneri); (3) frog (Xenopus); (4) chicken (Gallus gallus); (5) rat (Rattus); (6) cow (Bos taunts); (7) dog (Canis f a m i h i s ) ; (8) African green monkey (Cercopithecus aethiops). 1 2 3 4 5 6 7 8 9 101112131415161718 [880][881][882][883][884] with clear differences between the species evident by visual inspection.…”