Application of Elimination Voltammetry to the Resolution of Adenine and Cytosine Signals in Oligonucleotides II. Hetero‐Oligodeoxynucleotides with Different Sequences of Adenine and Cytosine Nucleotides

Trnková, Libuše; Jelen, František; Postbieglová, Irena

doi:10.1002/elan.200503447

Cited by 30 publications

(33 citation statements)

References 33 publications

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“…1a. In agreement with previous results [16] the EVLS curves show two separated reduction peaks. The dependence of the peak-counterpeak distance (the sum of peak height, I p , and counter peak height, I cp ) for A and C on accumulation time indicates the dynamics of adsorption process of ODN on electrode surface.…”

Section: Resultssupporting

confidence: 94%

“…In the case of adsorbed electroactive species this elimination function yields a specific peak-counterpeak signal [14 -16, 33, 35] in agreement with the theoretical derivation [16,33,35]. From the point of view of quantitative analysis this signal is very useful because it is well readable without application of base line correction [35].…”

Section: Theorymentioning

confidence: 55%

“…The elimination voltammetry with linear scan (EVLS) has been successfully applied to data analysis of oligodeoxynucleotides (ODNs) signal, aiming at better understanding of basic electrochemical processes on working electrode surfaces, and for better resolution of voltammetric signals of A and C in ODNs [14,16,32]. Using hanging mercury drop electrode (HMDE), A and C produce closely lying, overlapping reduction cathodic signals.…”

Section: Introductionmentioning

confidence: 99%

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Resolution of Overlapped Reduction Signals in Short Hetero‐oligonucleotides by Elimination Voltammetry

Mikelová¹,

Trnková²,

Jelen³

et al. 2007

Electroanalysis

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The resolution of overlapped reduction voltammetric signals of adenine (A) and cytosine (C) residues in short synthetic hetero-oligodeoxynucleotides (ODNs) with different sequences of A and C, but with the same ratio (A/C ¼ 1/2), was carried out by elimination voltammetry with linear scan (EVLS). The EVLS, as a mathematical transformation of voltammetric total currents measured at different scan rates, is capable to yield very sensitive resolution of potentially closed signals, which cannot be separated by common electrochemical methods. The best resolution of A and C signals was observed when the EVLS function eliminating the charging and kinetic currents (I c , I k ) and conserving the diffusion current (I d ) was combined with adsorption stripping technique (AdS). The resulting peak-counterpeak signal made it possible not only to separate the responses of A and C residues, but also to follow the dynamics of adsorption processes on mercury electrode surfaces. Together with the effect of the accumulation time on peak-counterpeak signal, the effect of pH and temperature on this signal was studied. The relation between elimination signals and the structure changes of ODNs was discussed.

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Section: Resultssupporting

confidence: 94%

Section: Theorymentioning

confidence: 55%

Section: Introductionmentioning

confidence: 99%

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Resolution of Overlapped Reduction Signals in Short Hetero‐oligonucleotides by Elimination Voltammetry

Mikelová¹,

Trnková²,

Jelen³

et al. 2007

Electroanalysis

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“…If a substance is adsorbed on an electrode and no chemical reaction occurs, the electrode process is controlled by adsorption, and the dependence of the capacitive (charging) current on the scan rate shows a linear relationship. This was verified on the reduction signals of adenine and cytosine in oligonucleotides and DNA adsorbed on mercury electrodes [13,19,28].…”

Section: Total and Partial Voltammetric Currentsmentioning

confidence: 99%

Elimination Procedure as a Novel and Promising Mathematical Approach in Voltammetric Methods

2010

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In the contribution, it has been demonstrated that Elimination Voltammetry with Linear Scan (EVLS) introduces an enhancement of the linear sweep and/or cyclic voltammetric results, and provides information about the type of the currents involved in the considered process. An extension of EVLS has been developed for any combination of scan rates (integers) for six elimination functions that are capable of conserving or eliminating of some voltammetric current components. Simple procedure to obtain the necessary coefficients from the chosen scan rates has been reported. In addition, the calculation and discussion of the relative error of elimination function (REEF) have been presented. The verification of the presented calculations has been done by studying different ratios of scan rates for reduction and oxidation processes of Cd(II) at a hanging mercury drop electrode (HMDE).

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“…The height and potential of signals studied were affected by the dC 9 /dA 9 ratio, time of accumulation, stirring speed during adsorption, and pH. Quite recently we have shown that EVLS in combination with adsorptive stripping procedure can reflect small differences in the oligonucleotide (ODN) sequences and distinguish adjacent and nonadjacent bases in ODN chain, which offers a new tool for the resolution of A and C in single-stranded DNA fragments [15]. The best resolution of A and C by EVLS can be achieved in the case of ODN in which sequences of As and Cs were in block arrangement [15 -17].…”

Section: Introductionmentioning

confidence: 99%

Double Elimination Voltammetry of Short Oligonucleotides

2007

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In connection with adsorptive stripping (AdS) technique, elimination voltammetry with linear scan (EVLS) in double mode was successfully employed in the analysis of homo-ODNs: 5'-AAA AAA AAA-3' (dA 9 ) and 5'-CCC CCC CCC-3' (dC 9 ), and hetero-ODNs: 5'-CCC AAA CCC-3' (H3), 5'-CAC CAC CAC-3' (H4), and 5'-ACC CAC CCA-3' (H9) on hanging mercury drop electrode (HMDE). Analogously to single EVLS function E4 (conserving the diffusion current I d and eliminating kinetic and charging currents I k , I c ) for the electroactive substance adsorbed, the double EVLS function E4 yields well readable peak-counterpeaks of reducible nucleic acid bases (adenine A and cytosine C). In comparison with single EVLS these peak-counterpeaks are higher by more than one order of magnitude (twenty times for dA 9 , fourteen times for dC 9 , from eight to sixteen times for hetero-ODNs). The increase of reduction signals with higher resolution was also observed using other two EVLS functions in double mode, the functions E5 eliminating I c and I d , but conserving I k and E6 eliminating I d and I k , but conserving I c . The amplifications of double / single EVLS signals of A and C range from 3.4 to 8.4, and from 3.1 to 8.3 for E5 and E6, respectively. It was proved that AdS double EVLS offers a new, fast, simple and inexpensive electroanalytical tool, which can be considered as a device not only for very good resolution of A and C in ODNs, but also for sensitive detection of changes in the primary structure of nucleic acid bases in ODN chains, depending on experimental conditions, such as pH, temperature, and time and potential of accumulation.

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Application of Elimination Voltammetry to the Resolution of Adenine and Cytosine Signals in Oligonucleotides II. Hetero‐Oligodeoxynucleotides with Different Sequences of Adenine and Cytosine Nucleotides

Cited by 30 publications

References 33 publications

Resolution of Overlapped Reduction Signals in Short Hetero‐oligonucleotides by Elimination Voltammetry

Resolution of Overlapped Reduction Signals in Short Hetero‐oligonucleotides by Elimination Voltammetry

Elimination Procedure as a Novel and Promising Mathematical Approach in Voltammetric Methods

Double Elimination Voltammetry of Short Oligonucleotides

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