Application of electron beam irradiation for improving the microbial quality of processed laver products and luminescence detection of irradiated lavers

Lee, Eun-Jin; Kim, Gui‐Ran; Ameer, Kashif; Kyung, Hyun-Kyu; Kwon, Joong‐Ho

doi:10.1007/s13765-017-0338-0

Cited by 9 publications

(7 citation statements)

References 16 publications

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“…A 4 kGy treatment achieved a 1.4 log CFU/g reduction of APC from dried laver [136]. Using heat-assisted low dose e-beam irradiation, coliforms could be eliminated from dried laver (> 1.5 log reduction) by 1-4 kGy without any changes in color or pigment contents [137,138]. Optimal treatment conditions ensuring product quality (i.e., thermophilic acidophilic bacterial count: 10 3 CFU/g; water content: 5%; acceptable palatability score from sensory evaluation) were suggested by response surface analysis as an irradiation dose of 1.8-3.0 kGy and heating at 154-170 • C for 10-18 s [139].…”

Section: Control Of Microbiological Risksmentioning

confidence: 99%

Health Functionality and Quality Control of Laver (Porphyra, Pyropia): Current Issues and Future Perspectives as an Edible Seaweed

Cho

Rhee

2019

Marine Drugs

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The growing interest in laver as a food product and as a source of substances beneficial to health has led to global consumer demand for laver produced in a limited area of northeastern Asia. Here we review research into the benefits of laver consumption and discuss future perspectives on the improvement of laver product quality. Variation in nutritional/functional values among product types (raw and processed (dried, roasted, or seasoned) laver) makes product-specific nutritional analysis a prerequisite for accurate prediction of health benefits. The effects of drying, roasting, and seasoning on the contents of both beneficial and harmful substances highlight the importance of managing laver processing conditions. Most research into health benefits has focused on substances present at high concentrations in laver (porphyran, Vitamin B 12 , taurine), with assessment of the expected effects of laver consumption. Mitigation of chemical/microbiological risks and the adoption of novel technologies to exploit under-reported biochemical characteristics of lavers are suggested as key strategies for the further improvement of laver product quality. Comprehensive analysis of the literature regarding laver as a food product and as a source of biomedical compounds highlights the possibilities and challenges for application of laver products.

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Section: Control Of Microbiological Risksmentioning

confidence: 99%

Health Functionality and Quality Control of Laver (Porphyra, Pyropia): Current Issues and Future Perspectives as an Edible Seaweed

Cho

Rhee

2019

Marine Drugs

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“…Assays were performed in a microtiter plate at a total volume of 250 μL per well. Each well contained 247 μL of medium and 3% methylene blue reaction mix [23]. The experiments were carried out in triplicate.…”

Section: Methodsmentioning

confidence: 99%

Sulfate assimilation regulates hydrogen sulfide production independent of lifespan and reactive oxygen species under methionine restriction condition in yeast

Choi

Kim

et al. 2019

Aging

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Endogenously produced hydrogen sulfide was proposed to be an underlying mechanism of lifespan extension via methionine restriction. However, hydrogen sulfide regulation and its beneficial effects via methionine restriction remain elusive. Here, we identified the genes required to increase hydrogen sulfide production under methionine restriction condition using genome-wide high-throughput screening in yeast strains with single-gene deletions. Sulfate assimilation-related genes, such as MET1 , MET3 , MET5 , and MET10 , were found to be particularly crucial for hydrogen sulfide production. Interestingly, methionine restriction failed to increase hydrogen sulfide production in mutant strains; however, it successfully extended chronological lifespan and reduced reactive oxygen species levels. Altogether, our observations suggested that increased hydrogen sulfide production via methionine restriction is not the mechanism underlying extended yeast lifespan, even though increased hydrogen sulfide production occurred simultaneously with yeast lifespan extension under methionine restriction condition.

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“…Each 1 g of the broken pieces received 9 mL of PBS solution to be shaken gently a few times. Then, each of the PBS solution was serially diluted, to be spread onto LB agar to count the colony forming unit (CFU) [13,14], by which immobilizing capacity (CFU/g of dry EC) of expanded clay was calculated.…”

Section: Performance Tests Of Bacteria-immobilized Expanded Clay As Cmentioning

confidence: 99%

Effectiveness of expanded clay as a bacteria carrier for self-healing concrete

et al. 2019

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Cracking of concrete over time, is a natural phenomenon. Longer service life of concrete structures is desirable. Self-healing concrete using bacteria, which could form CaCO 3 crystals for crack sealing, has promised benefits to reduce cost for concrete maintenance, because cracks could be autonomously repaired without human intervention. However, because of harsh concrete internal environment render the effectiveness depending on the bacteria viability within concrete. In this study, expanded clay (EC) was used as a carrier, to protect bacteria (Lysinibacillus boronitolerans YS11) from the harsh environment during the process. Existence of bacteria inside EC was observed using electron microscopy. When exposed to bacterial solution of 1.0 × 10 9 cells/mL, bacterial density within EC reached approximately 0.82 × 10 7 cells/g of dry EC. Extent of bacterial viability within EC, submerged to solution containing 1.0 × 10 8 cells/mL, was 53.6% of free bacteria solution containing 1.0 × 10 7 cells/mL, as measured with fluorescein diacetate assay. When rate of calcium carbonate formation was measured with Ca 2+ disappearance, rates were comparable between bacteria within EC (submerged to bacterial solution containing 1.0 × 10 8 cells/mL) and free bacteria (1.0 × 10 7 cells/mL). This finding indicates that bacteria with EC is very active for generation of CaCO 3 within EC. All experimental results suggest that EC may be an adequate bacteria carrier for self-healing concrete.

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Application of electron beam irradiation for improving the microbial quality of processed laver products and luminescence detection of irradiated lavers

Cited by 9 publications

References 16 publications

Health Functionality and Quality Control of Laver (Porphyra, Pyropia): Current Issues and Future Perspectives as an Edible Seaweed

Health Functionality and Quality Control of Laver (Porphyra, Pyropia): Current Issues and Future Perspectives as an Edible Seaweed

Sulfate assimilation regulates hydrogen sulfide production independent of lifespan and reactive oxygen species under methionine restriction condition in yeast

Effectiveness of expanded clay as a bacteria carrier for self-healing concrete

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