Application of DNA Fingerprinting to Enforcement of Hunting Regulations in Ontario

Guglich, Elizabeth A.; Wilson, Paul J.; White, Bradley N.

doi:10.1520/jfs13375j

Cited by 20 publications

(16 citation statements)

References 17 publications

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“…A beach seine was pulled through each site to randomly collect fish. Heads were removed, and the bodies placed individually in lysis buffer (Guglich et al, 1993). The Fish Springs Lagoon samples (n=24) were collected from a population numbering in the thousands.…”

Section: Sample Collectionmentioning

confidence: 99%

Genetic structure of Lake Magadi tilapia populations

Wilson

Wood

Maina

et al. 2000

Journal of Fish Biology

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The control region of the mitochondrial DNA haplotype frequencies were significantly different among the two separate lagoon populations of Oreochromis alcalicus grahami in Lake Magadi and of O. a. alcalicus from lake Natron, and DNA fingerprint similarity indices were significantly higher for intra-population comparisons of the two Magadi lagoon populations and the Lake Natron population than the inter-population similarity indices among these populations. A modified F st measure indicated population sub-division and the phylogeographic partitioning of the VNTR fragments observed were unique to specific populations further indicating substantial genetic differentiation. The lagoon populations within Lake Magadi demonstrated the same degree of genetic differentiation as either of these populations did to the outgroup (the Lake Natron population). There appears to be limited gene flow between Lake Magadi tilapia populations and this population structure has important implications for protecting locally adapted populations within this unique ecosystem. 2000 The Fisheries Society of the British Isles

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Section: Sample Collectionmentioning

confidence: 99%

Genetic structure of Lake Magadi tilapia populations

Wilson

Wood

Maina

et al. 2000

Journal of Fish Biology

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“…DNA analysis has been also applied to species identification (Tajima et al 1989;Naito et al 1992;Guglich et al 1993Guglich et al , 1994Stucki et al 1993;Oorschot et al 1994;Soteriou et al 1995;Sparkes et al 1996). From the view point of difference in the Alu and Myo loci, human DNA could be discriminated from mouse, rat, dog and deer DNA, but not from Japanese monkey or chimpanzee DNA, since there was hardly any difference in the Alu and Myo repeated sequences between human and Japanese monkey or chimpanzee (Tajima et al 1990).…”

Section: Discussionmentioning

confidence: 99%

“…With the advance of DNA analysis, genedetection procedures using DNA fingerprints or PCR have also been available for species identification (Tajima et al 1989;Blackett and Keim 1992;Guglich et al 1993Guglich et al , 1994Soteriou et al 1995). In the ABO blood group system, A, B and H antigens were found on the red blood cells of pongidae, old world monkeys and new world monkeys (Landsteiner and Miller 1925a, b;Candela 1940).…”

Section: Introductionmentioning

confidence: 99%

Species identification based on the point mutations of histo-blood group ABO genes by PCR-RFLP and direct sequencing

Wang

Ohshima

Kondo

1997

International Journal of Legal Medicine

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Using the PCR-restriction fragment length polymorphism (RFLP) and direct sequencing methods, 181 bp DNA fragments at the ABO blood group locus of human and eight different primates were examined. Through PCR amplification and digestion of the product with Hha-1 restriction enzyme, each of the amplified fragments of human, chimpanzee and green monkey was cut into two fragments of 147 and 34 bp, and the corresponding fragment of the other primates was digested into three fragment of 115, 34 and 32 bp. The 181 bp fragments of chimpanzee and green monkey were digested with Mva-1 into three fragments of 82, 58 and 41 bp, and 69, 58 and 54 bp, while the fragments of human and the other primates were separated into two fragments of 123 and 58 bp. Thus, using Hha-1 and Mva-1, human PCR-amplified product of a part of the ABO gene could be discriminated from those of the other primates examined. In addition, by direct sequencing of the 181 bp DNA fragment, two Mva-1 recognition sites and one Hha-1 recognition site were found in the fragments of chimpanzee and green monkey, one Mva-1 site and one Hha-1 site were detected in humans, and one Mva-1 site and two Hha-1 sites in the other primates. These results corresponded well with the data of PCR-RFLP. This method has a good potential for species identification at the DNA level.

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“…[5] It is very important to specify and identify individuals in Caretta caretta populations in order to estimate and try to prevent destructive behavior against those migratory species. [6] New molecular techniques make possible individual identification, [7] species identification, [8,9] gender identification, and population origin. [10,11] Caretta carettas are illegally traded themselves or their products like meat, eggs, shells or cosmetics containing turtle oils.…”

Section: Introductionmentioning

confidence: 99%

The Use of DNA Microsatellite Loci for “Caretta Caretta” Identification

Uysal

Petridis

Ozcan

et al. 2006

Journal of Environmental Science and Health, Part A

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Caretta carettas, one of the members of Chelonidae family, live primarily in water, except the periods of their ovulation where they come out to the shores to lay their eggs. Following an incubation period of 50-60 days, the newborns return to sea water to continue their 10-12-year life. Studies using marking methodology of the animals have shown that females return to the same place for ovulation every 2-3 years. The development of molecular genetic studies gives us now opportunity to trace all these routes that Caretta carettas follow during their life cycle. One of the basic techniques that is used for identification in general is the polymorphic DNA microsatellite loci. These 2-4 base pair DNA segments are considered to be ideal for Caretta caretta identification also. In this study we tried to establish a protocol in order to identify both male and female carettas in tissue samples collected from animals in the Mediterranean shoreline in the southern Turkey once this shoreline is one of the main spots of them for ovulation. The sampling has been done from 89 locations (Dalyan, Dalaman, Fethiye, Patara, Kale, Kumluca, Belek, Kizilot, Demirtaş, Gazipaşa and Anamur) from 246 dead baby Caretta carettas. DNA was extracted using silica-based extraction technology from the tissue homogenates. Cc7 locus was selected for identification to be tested for its degree of polymorphic content and therefore power of discrimination in general. The methodology used is PCR, followed by polyacrilamide gel electrophoresis and silver staining.

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Application of DNA Fingerprinting to Enforcement of Hunting Regulations in Ontario

Cited by 20 publications

References 17 publications

Genetic structure of Lake Magadi tilapia populations

Genetic structure of Lake Magadi tilapia populations

Species identification based on the point mutations of histo-blood group ABO genes by PCR-RFLP and direct sequencing

The Use of DNA Microsatellite Loci for “Caretta Caretta” Identification

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