Application of DNA-based genotyping techniques for the detection of kdr-like pyrethroid resistance in field populations of Colorado potato beetle

Kim, Hyo Jeong; Hawthorne, David J.; Peters, Todd E.; Dively, Galen P.; Clark, J. Marshall

doi:10.1016/j.pestbp.2004.10.002

Cited by 28 publications

(21 citation statements)

References 15 publications

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“…The sex linkage and polygenic effects on permethrin resistance observed in earlier studies was confirmed using quantitative trait locus mapping (Hawthorne 2003), and genotyping tests for the locus with the strongest effect have been developed (Kim et al 2005). A mutational change in the acetylcholine receptor of azinphosmethyl resistant potato beetles was sequenced (Zhu and Clark 1997).…”

Section: Genetics Of Resistancementioning

confidence: 82%

“…In a permethrin-resistant Colorado potato beetle strain, a single base pair mutation (C to T) resulted in an amino acid change (leucine to phenylalanine, L1014F) in an α-subunit of the sodium channel. That mutation was the major factor responsible for nerve insensitivity in Colorado potato beetles when exposed to permethrin (Lee et al 1999;Kim et al 2005). Also, nerve recording suggested that insensitivity at the target site may be one of the mechanisms of beetle resistance to imidacloprid (Tan et al 2005).…”

Section: Mechanisms Of Resistancementioning

confidence: 97%

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Colorado Potato Beetle Resistance to Insecticides

et al. 2008

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Section: Genetics Of Resistancementioning

confidence: 82%

Section: Mechanisms Of Resistancementioning

confidence: 97%

Colorado Potato Beetle Resistance to Insecticides

et al. 2008

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“…In addition, collecting large numbers of live specimens, particularly in case of lice, is impractical and always difficult. To circumvent these limitations, various individual genotyping techniques for the detection of resistance allele frequencies using genomic DNA extracted from target insects have been employed as alternative resistance monitoring tool (Clark et al, 2001; Kim et al, 2005). For the efficient monitoring of head lice resistance in the field based on the kdr genotype, we have developed a sequence of molecular tools, including quantitative sequencing (QS), real-time PCR amplification of specific allele (rtPASA) and serial invasive signal amplification reaction (SISAR).…”

Section: Mechanisms Of Kdrmentioning

confidence: 99%

Determination, mechanism and monitoring of knockdown resistance in permethrin-resistant human head lice, Pediculus humanus capitis

Clark

2009

Journal of Asia-Pacific Entomology

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Permethrin resistance has been reported worldwide and clinical failures to commercial pediculicides containing permethrin have likewise occurred. Permethrin resistance in head lice populations from the U.S. is widespread but is not yet uniform and the level of resistance is relatively low (~4-8 fold). Permethrin-resistant lice are cross-resistant to pyrethrins, PBO-synergized pyrethrins and to DDT. Nix ® , when applied to human hair tufts following manufacture's instructions, did not provide 100% control when assessed by the hair tuft bioassay in conjunction with the in vitro rearing system. Resistance to permethrin is due to knockdown resistance (kdr), which is the result of three point mutations within the α-subunit gene of the voltage-gated sodium channel that causes amino acid substitutions, leading to nerve insensitivity.A three-tiered resistance monitoring system has been established based on molecular resistance detection techniques. Quantitative sequencing (QS) has been developed to predict the kdr allele frequency in head lice at a population level. The speed, simplicity and accuracy of QS made it an ideal candidate for a routine primary resistance monitoring tool to screen a large number of louse populations as an alternative to conventional bioassay. As a secondary monitoring method, real-time PASA (rtPASA) has been devised for a more precise determination of low resistance allele frequencies. To obtain more detailed information on resistance allele zygosity, as well as allele frequency, serial invasive signal amplification reaction (SISAR) has been developed as an individual genotyping method. Our approach of using three tiers of molecular resistance detection should facilitate large-scale routine resistance monitoring of permethrin resistance in head lice using fieldcollected samples. KeywordsPediculus humanus capitis; Permethrin resistance; Quantitative sequencing; real time PCR amplification of specific alleles; serial invasive signal amplification reaction *Corresponding author. Fax: 413 577 4267, jclark@vasci.umass.edu (J.M. Clark). Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. and Harwood, 1969) and are so closely related that a long debate has occurred about whether they are subspecies or two separate species (Kitter et al., 2003;Yong et al., 2003) Body lice pose a serious public health threat because they transmit several types of bacteria (Rickettsia prowazekii, Borrelia recurrentis and Borrelia recurrentis) that cause human diseases (epidemic typhus, louse-borne relapsing fever, five-day relapsing fever or trench fever) and these diseases ...

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“…The The PCR-SSCP method for molecular analysis of genetic diseases (β-thalassaemia, cystic fibrosis, β-globin) in humans have been well documented in clinical genetics [11]. SSCP has been also used for detection of pyrethroid resistance [12] and acetylcholinesterase resistance [13] in populations of Colorado potato beetle. The reliability and accuracy of this technique to monitor resistant and susceptible allele frequencies in insect population was found to be 95 % [12].…”

Section: Resultsmentioning

confidence: 99%

“…SSCP has been also used for detection of pyrethroid resistance [12] and acetylcholinesterase resistance [13] in populations of Colorado potato beetle. The reliability and accuracy of this technique to monitor resistant and susceptible allele frequencies in insect population was found to be 95 % [12]. In plants, SSCP has been used to detect acetohydroxyacid synthase (AHAS) mutations conferring resistance to imidazolinone or sulfonylurea herbicides in wild biotypes of cultivated sunflower.…”

Section: Resultsmentioning

confidence: 99%

Molecular Genotyping of Herbicide Resistance in P. minor: ACCase Resistance

Singh

Sharma

Raghav

et al. 2014

Appl Biochem Biotechnol

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Little seed canary grass (Phalaris minor Retz.) populations resistant to herbicides that inhibit acetyl-CoA carboxylase (ACCase) represent an increasingly important weed control problem in northern India. The objective of this study was to develop DNA-based markers to differentiate herbicide-resistant and herbicide-susceptible population of P. minor. Primers were designed to amplify the conserved region carrying two reported mutations Trp2027 to Cys and Ile2041 to Asn conferring ACCase inhibitor resistance in several grass weeds and subjected to single-strand conformational polymorphism (SSCP) to detect the mutations. Five distinctive electrophoretic patterns on non-denaturing PAGE were observed, and four patterns were found to be associated with ACCase herbicide resistance in P. minor. The PCR-SSCP test developed in this study confirmed 17 resistant populations to contain mutations in CT domain of ACCase gene. This is the first report of rapid and easy molecular diagnosis of ACCase herbicide-resistant and herbicide-sensitive population of P. minor through PCR-SSCP analysis.

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Application of DNA-based genotyping techniques for the detection of kdr-like pyrethroid resistance in field populations of Colorado potato beetle

Cited by 28 publications

References 15 publications

Colorado Potato Beetle Resistance to Insecticides

Colorado Potato Beetle Resistance to Insecticides

Determination, mechanism and monitoring of knockdown resistance in permethrin-resistant human head lice, Pediculus humanus capitis

Molecular Genotyping of Herbicide Resistance in P. minor: ACCase Resistance

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