Application of DNA Array Technology for Diagnostic Microbiology

Booth, Stephanie A.; Drebot, Michael A.; Ng, L.-K.

doi:10.1155/2000/127160

Cited by 2 publications

(1 citation statement)

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“…We next used this strategy to specifically release captured oligonucleotides from DNA microarrays, which are commonly used to capture RNA, DNA, or proteins from solution for various types of analyses 16, 17 . DNA microarrays have also been used in enzymatic reactions enabling diverse technologies such as gene assembly and DNA computation 18–20 .…”

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confidence: 99%

Multiplexed Programmable Release of Captured DNA

et al. 2014

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Nucleic acid hybridization is widely used for the specific capture of complementary sequences from complex samples. It is useful for both analytical methodologies, such as array hybridization (e.g. transcriptome analysis, genetic variation analysis), and preparative strategies such as exome sequencing and sequence-specific proteome capture and analysis (PICh, HyCCAPP). It has not generally been possible to selectively elute particular captured subsequences, however, as the conditions employed for disruption of a duplex can lack the specificity needed to discriminate between different sequences. We show here that it is possible to bind and selectively release multiple sets of sequences by using toehold-mediated DNA branch migration. The strategy is illustrated for simple mixtures of oligonucleotides, for the sequence-specific capture and specific release of crosslinked yeast chromatin, and for the specific release of oligonucleotides hybridized to DNA microarrays.

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confidence: 99%