Application of disease-associated differentially expressed genes – Mining for functional candidate genes for mastitis resistance in cattle

Abstract

In this study the mRNA differential display method was applied to identify mastitis-associated expressed DNA sequences based on different expression patterns in mammary gland samples of non-infected and infected udder quarters of a cow. In total, 704 different cDNA bands were displayed in both udder samples. Five hundred-and-thirty two bands, (75.6%) were differentially displayed. Ninety prominent cDNA bands were isolated, re-amplified, cloned and sequenced resulting in 87 different sequences. Amongst the 19 expressed sequence tags showing a similarity with previously described genes, the majority of these sequences exhibited homology to protein kinase encoding genes (26.3%), to genes involved in the regulation of gene expression (26.3%), to growth and differentiation factor encoding genes (21.0%) and to immune response or inflammation marker encoding genes (21.0%). These sequences were shown to have mastitis-associated expression in the udder samples of animals with and without clinical mastitis by quantitative RT-PCR. They were mapped physically using a bovine-hamster somatic cell hybrid panel and a 5000 rad bovine whole genome radiation hybrid panel. According to their localization in QTL regions based on an established integrated marker/gene-map and their disease-associated expression, four genes (AHCY, PRKDC, HNRPU, OSTF1) were suggested as potentially involved in mastitis defense.

Application of Selection Mapping to Identify Genomic Regions Associated with Dairy Production in Sheep

“…The POFUT1 (protein O-fucosyltransferase 1) gene plays a crucial role in Notch signaling, which regulates mammary stem cell function and luminal cell-fate commitment [44] . TFAP2C (transcription factor AP-2 gamma; activating enhancer binding protein 2 gamma) is involved in mammary development, differentiation, and oncogenesis playing a critical role in gene regulation in hormone responsive breast cancer [45] , and AHCY (adenosylhomocysteinase) has been suggested as potentially involved in mastitis defense based on its disease-associated expression [46] . Another positional candidate gene for this core region is the GHRH (growth hormone-releasing hormone) gene.…”

Linkage disequilibrium and genomic scan to detect selective loci in cattle populations adapted to different ecological conditions in Ethiopia

“…The absence of a functioning DNA-PK is known to be associated with down-regulation of heat shock protein 70 (HSP70) (Okazawa et al 2013). This gene has also been identified as one of the functional candidates for mastitis resistance in cattle (Schwerin et al 2003).…”

Transcriptomic analysis of milk somatic cells in mastitis resistant and susceptible sheep upon challenge with Staphylococcus epidermidis and Staphylococcus aureus

“…The involvement of AHR in the control of inflammatory responsiveness has been reported previously [ 44 ]. To our knowledge the role of TP53 in the response to bacterial infection has yet to be studied whereas it has been shown elsewhere to be associated with mastitis infected quarters [ 45 ]. tp53 was the gene that displayed the second highest ratio between lines and was over-expressed in the resistant line; it was highly associated with resistant animals in PCA and was represented as a hub in IPA networks since it interacts with numerous other DE genes.…”