Application of design of experiments and design space methodology for the HPLC-UV separation optimization of aporphine alkaloids from leaves of Spirospermum penduliflorum Thouars

Rafamantanana, Mamy; Debrus, Benjamin; Raoelison, Guy; Rozet, Eric; Lebrun, Philippe; Uverg-Ratsimamanga, Suzanne; Hubert, Philippe; Quetin-Leclercq, Joëlle

doi:10.1016/j.jpba.2011.12.028

Cited by 26 publications

(16 citation statements)

References 25 publications

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“…aruata [3,4], and validated HPLC methods of determination of aporphine alkaloids [5][6][7]. As for high performance liquid chromatography/multi-stage mass spectrometry (HPLC-MSn) approach, a powerful technique for TCM (7), (+)-isoboldine (8), (+)-isocorydine (9), (+)-laurotetanine (11), and (+)-N-methyllaurotetanine (12).…”

Section: Introductionmentioning

confidence: 99%

Characterization and simultaneous quantification of biological aporphine alkaloids in Litsea cubeba by HPLC with hybrid ion trap time‐of‐flight mass spectrometry and HPLC with diode array detection

Zhang

Guo

et al. 2015

J of Separation Science

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The root and rhizome of Litsea cubeba (Lour) Pers., named 'Dou-chi-jiang' in Chinese, has been traditionally used for treatment of cardiovascular and cerebrovascular diseases, rheumatic arthralgia, and other diseases in China. Aporphine alkaloids are its characteristic ingredients and responsible for its bioactivities, especially anti-inflammatory and analgesic effects. A sensitive and reliable high-performance liquid chromatography with diode array detection-tandem mass spectrometry method was developed for characterization and simultaneous determination of biological aporphine alkaloids in 'Dou-chi-jiang'. The optimized chromatographic conditions were performed on an Eclipse XDB C18 column with a gradient of acetonitrile/water containing 0.1% formic acid as the mass spectrometry mobile phase and acetonitrile/water containing 0.2% diethylamine (pH 3.10, adjusted by acetic acid) as the liquid chromatography mobile phase. The fragmentation pathways by loss of CO, ·CH3 , ·NH3 , and ·NH2 CH3 were detected as characteristic for aporphine alkaloids. Based on these characteristics, total 12 analogues were identified. The quantification method was validated in terms of linearity, precision, and accuracy for six major aporphine alkaloids, which was successfully applied for simultaneous determination in ten batches of samples. The established method is simple, rapid, and specific for characterization and quantitation of aporphine alkaloids in 'Dou-chi-jiang' and other traditional Chinese medicines rich in this kind of ingredient.

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Section: Introductionmentioning

confidence: 99%

Characterization and simultaneous quantification of biological aporphine alkaloids in Litsea cubeba by HPLC with hybrid ion trap time‐of‐flight mass spectrometry and HPLC with diode array detection

Zhang

Guo

et al. 2015

J of Separation Science

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“…Hypophorine (5) is an indole alkaloid isolated from Erythrina lysistemon, a leguminous plant harvested in Botswana. 40 Both dicentrine and neolitsine are known to possess antihypertensive activities, dicentrine having an EC 50 value of 0.15 AE 0.04 mg mL À1 on rat aorta relaxation. 34,35 Although erythrinaline alkaloids 36,37 and prenylated avonoids are known to be prevalent in the plant species, 33,38 compound 5 is known to contribute to its antimicrobial activities.…”

Section: Alkaloids From Southern African Floramentioning

confidence: 99%

“…33 The extracts from this plant have been used in traditional medicine and have also shown antiviral, anticancer and cytotoxic activities. 40 A review on plants traditionally used for the treatment of malaria in Madagascar, showed Vepris ampody as a key component in anti-malarial preparations in Madagascan traditional medicine. 33 Spirospermum penduliorum (Menispermaceae) is endemic in Madagascar.…”

Section: Alkaloids From Southern African Floramentioning

confidence: 99%

The chemistry and bioactivity of Southern African flora I: a bioactivity versus ethnobotanical survey of alkaloid and terpenoid classes

et al. 2015

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As a whole, the African continent is highly endowed with a huge floral biodiversity. Natural products which have been isolated from plants growing in this region have shown interesting chemical structures with diverse biological activities, which could serve as a starting point for drug discovery. In this study, a literature survey led to the collection of 864 secondary metabolites from 101 plant species from 57 plant families. A correlation between the known biological activities of isolated compounds and the ethnobotanical uses of the plants has been attempted. This review is a survey of the bioactivities of alkaloids and terpenoids which have been isolated from Southern African flora versus the ethnobotanical uses of the plants used in Southern African traditional medicine.

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“…Applications for the development of analytical methods for the separation of compounds in complex plant extracts are also available in the literature [15][16][17]. They involved the separation of tertiary alkaloids [15] or aporphine alkaloids [17] in leaf extracts of various plants or the separation of cannabinoids [17] in various plant materials by HPLC-UV.…”

Section: "What Is Disturbing With Rs As a Modeled Response Is That Imentioning

confidence: 99%

New Methodology For The Development of Chromatographic Methods With Bioanalytical Application

et al. 2012

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Application of design of experiments and design space methodology for the HPLC-UV separation optimization of aporphine alkaloids from leaves of Spirospermum penduliflorum Thouars

Cited by 26 publications

References 25 publications

Characterization and simultaneous quantification of biological aporphine alkaloids in Litsea cubeba by HPLC with hybrid ion trap time‐of‐flight mass spectrometry and HPLC with diode array detection

Characterization and simultaneous quantification of biological aporphine alkaloids in Litsea cubeba by HPLC with hybrid ion trap time‐of‐flight mass spectrometry and HPLC with diode array detection

The chemistry and bioactivity of Southern African flora I: a bioactivity versus ethnobotanical survey of alkaloid and terpenoid classes

New Methodology For The Development of Chromatographic Methods With Bioanalytical Application

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