Application of Deoxyribonucleic Acid (DNA) Polymorphisms to the Analysis of DNA Recovered from Sperm

Abstract

Sperms, collected following sexual activity of volunteers, were processed to isolate high-molecular weight deoxyribonucleic acid (DNA). These DNA samples were digested with particular restriction endonucleases and analyzed with probes that recognize polymorphic DNA regions within the human genome. The pattern of restriction fragment length polymorphisms (RFLP) detected by this test is identical to that observed with DNA prepared from blood of the male sexual partner. Therefore, RFLP analysis can be used to exclude or to determine the probable identity of an assailant in rape cases.

Hypervariable polymorphic VNTR loci for parentage testing and individual identification

“…Now, it can be used without constraint of patents. The combination of single-locus DNA polymorphisms getting from VNTR loci proved very powerful informations for parentage testing and individual identification which has been pointed out by Giusti et aL (1986) and Wainscoat et al (1987). Using hypervariable VNTR loci, the power of parentage testing and individual identification are extremely increased compared with those obtained from classical methods.…”

“…Another class of polymorphisms in hypervariable regions of DNA which resulted from a variable number of tandem repeats (VNTR). VNTR loci are valuable genetic markers for human linkage maps , parentage testing (Jeffreys et al, 1985a;Baird et al, 1986) and individual identification (Jeffreys et a!., 1985b;Gill et al, 1985;Giusti et al, 1986). A common feature of these probes is the presence of a short core sequence element (i.e., VNTR or hypervariable repeat, HVR) which is repeated in tandem along the chromosome and provides for the variable polymorphic character the locus (Nakamura et al, 1987).…”

Individual identification of human bloodstains investigated with hypervariable DNA loci.

“…Hypervariable DNA loci have been widely applied, especially in individual identification (Gill et al 1985 ;Giusti et al 1986), and in paternity test (Jeffreys et al 1985a, b ;Baird et al 1986). Most of them are multi-locus probes that recognize several loci under reduced stringency.…”

“…; Allen et al 1989) and individual identification (Jeffreys et al 1985b; Gill et al 1985; Giusti et al 1986). In those reports multi-locus probes were used for individual identification, but statistical analyses were very difficult, and reproducible results were hard to get due to undefined chromosomal location and low stringency washing of hybridization filter (Olaisen et al 1987; Yokoi et al 1990a).…”

Investigation of paternity establishing without the putative father using hypervariable DNA probes

“…Hypervariable DNA polymorphisms has been widely applied, in individual identification (Jeffreys et al, 1985b;Gill et al, 1985;Giusti et al, 1986), and in paternity suits (Jeffreys et aI., 1985a;Baird et al, 1986;Allen et al, 1989). Most of them are multi-locus probes that recognize several loci under the conditions of reduced stringency.…”